Comparison of reversed-phase, hydrophilic interaction, and porous graphitic carbon chromatography columns for an untargeted toxicometabolomics study in pooled human liver microsomes, rat urine, and rat plasma.
Hydrophilic interaction chromatography columns
LC-HRMS
Quality assurance
Reversed-phase columns
Untargeted metabolomics
Journal
Metabolomics : Official journal of the Metabolomic Society
ISSN: 1573-3890
Titre abrégé: Metabolomics
Pays: United States
ID NLM: 101274889
Informations de publication
Date de publication:
30 Apr 2024
30 Apr 2024
Historique:
received:
12
10
2023
accepted:
20
04
2024
medline:
1
5
2024
pubmed:
1
5
2024
entrez:
30
4
2024
Statut:
epublish
Résumé
Untargeted metabolomics studies are expected to cover a wide range of compound classes with high chemical diversity and complexity. Thus, optimizing (pre-)analytical parameters such as the analytical liquid chromatography (LC) column is crucial and the selection of the column depends primarily on the study purpose. The current investigation aimed to compare six different analytical columns. First, by comparing the chromatographic resolution of selected compounds. Second, on the outcome of an untargeted toxicometabolomics study using pooled human liver microsomes (pHLM), rat plasma, and rat urine as matrices. Separation and analysis were performed using three different reversed-phase (Phenyl-Hexyl, BEH C All three reversed-phase columns showed a similar performance, whereas the PGC column was superior to both HILIC columns at least for polar compounds. Comparing the size of feature count across all datasets, most features were detected using the Phenyl-Hexyl or sulfobetaine column. Considering the matrices, most significant features were detected in urine and pHLM after using the sulfobetaine and in plasma after using the ammonium-sulfonic acid column. The results underline that the outcome of this untargeted toxicometabolomic study LC-HRMS metabolomic study was highly influenced by the analytical column, with the Phenyl-Hexyl or sulfobetaine column being the most suitable. However, column selection may also depend on the investigated compounds as well as on the investigated matrix.
Identifiants
pubmed: 38689195
doi: 10.1007/s11306-024-02115-0
pii: 10.1007/s11306-024-02115-0
doi:
Substances chimiques
Graphite
7782-42-5
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Comparative Study
Langues
eng
Sous-ensembles de citation
IM
Pagination
49Informations de copyright
© 2024. The Author(s).
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