The impact of continuous cultivation of Ganoderma lucidum on soil nutrients, enzyme activity, and fruiting body metabolites.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
02 May 2024
02 May 2024
Historique:
received:
18
02
2024
accepted:
26
04
2024
medline:
3
5
2024
pubmed:
3
5
2024
entrez:
2
5
2024
Statut:
epublish
Résumé
To explore the impacts of continuous Ganoderma lucidum cultivation on soil physicochemical factors, soil enzyme activity, and the metabolome of Ganoderma lucidum fruiting bodies, this study conducted two consecutive years of cultivation on the same plot of land. Soil physicochemical factors and enzyme activity were assessed, alongside non-targeted metabolomic analysis of the Ganoderma lucidum fruiting bodies under continuous cultivation. The findings unveiled that in the surface soil layer (0-15 cm), there was a declining trend in organic matter, ammonium nitrogen, available phosphorus, available potassium, pH, polyphenol oxidase, peroxidase, alkaline phosphatase, and sucrase, whereas nitrate nitrogen, electrical conductivity (EC), and salt content exhibited an upward trend. Conversely, in the deeper soil layer (15-30 cm), organic matter, ammonium nitrogen, available potassium, alkaline phosphatase, and sucrase demonstrated a decreasing trend, while nitrate nitrogen, available phosphorus, pH, EC, salt content, polyphenol oxidase, and soil peroxidase showed an increasing trend. Metabolomic analysis of Ganoderma lucidum fruiting bodies distinguished 64 significantly different metabolites between the GCK and GT groups, with 39 components having markedly higher relative contents in GCK and 25 components having significantly lower relative contents in GCK compared to GT. Moreover, among these metabolites, there were more types with higher contents in the fruiting bodies harvested in the first year (GCK) compared to those harvested in the second year (GT), with pronounced differences. KEGG pathway analysis revealed that GCK exhibited more complex metabolic pathways compared to GT. The metabolites of Ganoderma lucidum fruiting bodies were predominantly influenced by soil physicochemical factors and soil enzyme activity. In the surface soil layer (0-15 cm), the metabolome was significantly affected by soil pH, soil organic matter, available phosphorus, and soil alkaline phosphatase, while in the deeper soil layer (15-30 cm), differences in the Ganoderma lucidum metabolome were more influenced by soil alkaline phosphatase, soil catalase, pH, nitrate nitrogen, and soil sucrase.
Identifiants
pubmed: 38698154
doi: 10.1038/s41598-024-60750-y
pii: 10.1038/s41598-024-60750-y
doi:
Substances chimiques
Soil
0
Nitrogen
N762921K75
Phosphorus
27YLU75U4W
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
10097Subventions
Organisme : the Financial Grant Support Program of Lianyungang City, Jiangsu Province, China
ID : QNJJ2310
Organisme : the Second Comprehensive Scientific Expedition to the Qinghai-Tibet Plateau
ID : 2019QZKK0405
Organisme : the Qinghai Province key research and development and transformation plan
ID : 2022-QY-204
Organisme : the Qinghai Province science and technology plan
ID : 2023-ZJ-905T
Informations de copyright
© 2024. The Author(s).
Références
Tong, X., Jiang, H., Liang, Y., Rao, Y. & Wang, Y. Waterlogging reduces soil colonization by antagonistic fungi and restores production in Ganoderma lucidum continuous cultivation. Crop Prot. 137, 105314 (2020).
doi: 10.1016/j.cropro.2020.105314
Oke, M. et al. Ganoderma lucidum: Unutilized natural medicine and promising future solution to emerging diseases in Africa. Front. Pharmacol. 13, 952027 (2022).
doi: 10.3389/fphar.2022.952027
pubmed: 36071846
pmcid: 9441938
Taofiq, O. et al. The potential of Ganoderma lucidum extracts as bioactive ingredients in topical formulations, beyond its nutritional benefits. Food Chem. Toxicol. 108(Pt A), 139–147 (2017).
doi: 10.1016/j.fct.2017.07.051
pubmed: 28760544
Chen, X., Veena, R., Ramya, H., Janardhanan, K. & George, V. Gano oil: A novel antinociceptive agent extracted from Ganoderma lucidum inhibits paw oedema and relieves pain by hypnotic and analgesic actions of fatty acid amides. J. Ethnopharmacol. 263, 113144 (2020).
doi: 10.1016/j.jep.2020.113144
pubmed: 32730883
Sharif Swallah, M. et al. Potentialities of Ganoderma lucidum extracts as functional ingredients in food formulation. Food Res. Int. (Ottawa, Ont) 172, 113161 (2023).
doi: 10.1016/j.foodres.2023.113161
Zhang, H., Jiang, H., Zhang, X. & Yan, J. Amino acids from Ganoderma lucidum: Extraction optimization, composition analysis, hypoglycemic and antioxidant activities. Curr. Pharm. Anal. 14, 562 (2018).
doi: 10.2174/1573412913666170918161654
Tang, X. et al. Beneficial shift of rhizosphere soil nutrients and metabolites under a sugarcane/peanut intercropping system. Front. Plant Sci. 13, 1018727 (2022).
doi: 10.3389/fpls.2022.1018727
pubmed: 36531399
pmcid: 9757493
Zou, P. et al. Ganoderma lucidum autotoxicity of endogenous organic acid stress in two cultivars. Molecules (Basel, Switzerland) 27(19), 6734 (2022).
doi: 10.3390/molecules27196734
pubmed: 36235268
Jiang, A. L. et al. Integrated proteomics and metabolomics analysis provides insights into ganoderic acid biosynthesis in response to methyl jasmonate in Ganoderma Lucidum. Int. J. Mol. Sci. 20(24), 6116 (2019).
doi: 10.3390/ijms20246116
pubmed: 31817230
pmcid: 6941157
Liu, Y. et al. Ganoderma lucidum study of the metabolite changes in under pineapple leaf residue stress via LC-MS/MS coupled with a non-targeted metabolomics approach. Metabolites 13(4), 487 (2023).
doi: 10.3390/metabo13040487
pubmed: 37110146
pmcid: 10144527
Wu, X. et al. An integrated microbiome and metabolomic analysis identifies immunoenhancing features of Ganoderma lucidum spores oil in mice. Pharmacol. Res. 158, 104937 (2020).
doi: 10.1016/j.phrs.2020.104937
pubmed: 32464331
Meng, L. et al. Ganoderma lucidum integrated transcriptomics and nontargeted metabolomics analysis reveal key metabolic pathways in in response to ethylene. J. Fungi (Basel, Switzerland) 8(5), 456 (2022).
Diego, C. Z. & Pardo-Giménez, A. Cultivation of Ganoderma lucidum. In Edible and medicinal mushrooms (technology and applications) (Wiley, 2017). https://doi.org/10.1002/9781119149446:385-413 .
doi: 10.1002/9781119149446:385-413
Li, Y., Fang, F., Wei, J., Wu, X. & Tan, D. Humic acid fertilizer improved soil properties and soil microbial diversity of continuous cropping peanut: A three-year experiment. Sci. Rep. https://doi.org/10.1038/s41598-019-48620-4 (2019).
doi: 10.1038/s41598-019-48620-4
pubmed: 31892714
pmcid: 6938520
Li, J., Zhuoying, X., Yongbo, X., Xinhua, Y. & Xiyu, W. Effects of continuous lily cropping on the physicochemical properties and biological characteristics in subtropical facility red soils. Eurasian Soil Sci. 55, 1258 (2022).
doi: 10.1134/S1064229322090095
Barro, F., Fontes, A. G. & Maldonado, J. M. Organic nitrogen content and nitrate and nitrite reductase activities in tritordeum and wheat grown under nitrate or ammonium. Plant Soil 135(2), 251–256 (1991).
doi: 10.1007/BF00010913
Marisa, L. et al. KEGG: Kyoto Encyclopedia of Genes and Genomes. Nucleic Acids Res. 27(1), 29–34 (1999).
Kanehisa, M. Toward understanding the origin and evolution of cellular organisms. Protein Sci. 28(11), 1947 (2019).
doi: 10.1002/pro.3715
pubmed: 31441146
pmcid: 6798127
Minoru, K. et al. KEGG for taxonomy-based analysis of pathways and genomes. Nucleic Acids Res. 51, D587 (2022).
Arafat, Y. et al. Long-term monoculture negatively regulates fungal community composition and abundance of tea orchards. Agronomy 9(8), 466 (2019).
doi: 10.3390/agronomy9080466
Liu, H. et al. Response of soil fungal community structure to long-term continuous soybean cropping. Front. Microbiol. 9, 3316 (2019).
doi: 10.3389/fmicb.2018.03316
pubmed: 30687292
pmcid: 6333693
Fujii, K. et al. Continuous maize cropping accelerates loss of soil organic matter in northern Thailand as revealed by natural C-13 abundance. Plant Soil 474, 251. https://doi.org/10.1007/s11104-022-05333-4 (2022).
doi: 10.1007/s11104-022-05333-4
Ren, B. et al. Urea ammonium nitrate solution combined with urease and nitrification inhibitors jointly mitigate NH
doi: 10.1016/j.fcr.2023.108909
Che, Y., Guo, L. & Li, Z. Effects of continuous cropping on bacteria1 community and diversity in rhizosphere soi1 of industria1 hemp a five year experiment. Diversity 14, 250 (2022).
doi: 10.3390/d14040250
Ma, D. et al. Soil water and salt migration in oasis farmland during crop growing season. J Soils Sedi. 23, 355–367 (2023).
Wu, J. et al. Vegetation degradation impacts soil nutrients and enzyme activities in wet meadow on the Qinghai-Tibet Plateau. Sci. Rep. 10(1). 21271 (2020).
Xu, C. et al. Enhanced phytoremediation of PAHs-contaminated soil from an industrial relocation site by Ochrobactrum sp. Environ. sci. pollut. res. int. 27(9), 8991–8999 (2020).
Sun, Y. et al. Gold Nanoparticle-Protein Conjugate Dually-Responsive to pH and Temperature for Modulation of Enzyme Activity. J. Mater. Chem. B. 7, 3260–3267 (2019).
Kou, T. J. et al. Soil urease and catalase responses to ozone pollution are affected by the ozone sensitivity of wheat cultivars. J. Agron. Crop Sci. 204(4), 424–428 (2018).
Tan, X. et al. Temperature enhances the affinity of soil alkaline phosphatase to Cd. Chemosphere: Environ. toxicol. risk assess. 196, 214–222 (2018).
Qin, X. et al. Effects of Sepiolite and Biochar on Enzyme Activity of Soil Contaminated by Cd and Atrazine[J]. Bullet. Environ. Contam. Toxicol. 104(5), 642–648 (2020).
Zhao Y, et al. Soil metabolomics and bacterial functional traits revealed the responses of rhizosphere soil bacterial community to long-term continuous cropping of Tibetan barley. PeerJ. 10, e13254 (2022).
doi: 10.1080/10286020802240418
Smarrito, C. M. et al. A novel efficient and versatile route to the synthesis of 5-O-feruloylquinic acids. Organ. Biomol. Chem. 6(6), 986–987 (2008).
Yuan, Y. et al. Synthesis and enantiomeric resolution of (±)-pinocembrin. J. Asian Nat. Prod. Res. 10(10), 999–1002 (2008).
doi: 10.1080/10286020802240418
pubmed: 19003622
Fagboun, D. E. et al. Dihydrostilbene phytoalexins from Dioscorea rotundata. Phytochemistry 26(12), 3187–3189 (1987).
Lipeeva, A. V. et al. Design, Synthesis and Antibacterial Activity of Coumarin-1,2,3-triazole Hybrids Obtained from Natural Furocoumarin Peucedanin. Molecules 24(11), 2126 (2019).
Wang, Y. J. et al. Simultaneous determination of neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid and geniposide in rat plasma by UPLC-MS/MS and its application to a pharmacokinetic study after administration of Reduning injection. Biomed. Chromat. Inter. J. Devot. Res. Chromat. Methodol. Appli. Biosci. 29(1), 68–74 (2015).
Fu, C. et al. Oral Bioavailability Comparison of Artemisinin, Deoxyartemisinin, and 10-Deoxoartemisinin Based on Computer Simulations and Pharmacokinetics in Rats. ACS Omega 6(1), 889 (2020).
Yang, W. et al. Comparative Analysis of Rhizosphere and Endophytic Microbial Communities Between Root Rot and Healthy Root of Psammosilene tunicoides. Curr. Microbiol. 80(7), 215 (2023).