Effects of different extenders on epigenetic patterns and functional parameters of bull sperm during cryopreservation process.
Male
Cryopreservation
/ veterinary
Animals
Epigenesis, Genetic
Cattle
Spermatozoa
/ drug effects
Semen Preservation
/ veterinary
DNA Fragmentation
Sperm Motility
/ drug effects
Cryoprotective Agents
/ pharmacology
DNA Methylation
/ drug effects
Egg Yolk
/ chemistry
Lecithins
/ pharmacology
Histones
/ metabolism
Glycine max
/ chemistry
Semen Analysis
/ veterinary
Acetylation
bull sperm cryopreservation
egg yolk
epigenetic patterns
soybean lecithin
Journal
Reproduction in domestic animals = Zuchthygiene
ISSN: 1439-0531
Titre abrégé: Reprod Domest Anim
Pays: Germany
ID NLM: 9015668
Informations de publication
Date de publication:
May 2024
May 2024
Historique:
revised:
27
03
2024
received:
06
02
2024
accepted:
09
04
2024
medline:
3
5
2024
pubmed:
3
5
2024
entrez:
3
5
2024
Statut:
ppublish
Résumé
The cryopreservation process induces alterations in cellular parameters and epigenetic patterns in bull sperm, which can be prevented by adding cryoprotectants in the freezing extenders. The purpose of this study was to compare the protective effects of two extenders based on soybean lecithin (SLE) and egg yolk (EYE) on epigenetic patterns and quality parameters of sperm such as motility parameters, mitochondrial membrane integrity, DNA fragmentation, viability, and apoptotic-like changes of bull sperm after cryopreservation. Results demonstrated that cryopreservation significantly (p < .05) reduced the level of DNA global methylation, H3K9 histone acetylation, and H3K4 histone methylation in both frozen groups compared to the fresh sperm. Also, the level of H3K9 acetylation was lower in the frozen SLE group (21.2 ± 1.86) compared to EYE group (15.2 ± 1.86). In addition, the SLE frozen group had a higher percentage of viability, progressive motility, and linearity (LIN) in SLE frozen group compared to EYE frozen group. However, no difference was observed in mitochondrial membrane integrity and DNA fragmentation between SLE and EYE frozen groups. While soybean-lecithin-based extender showed some initial positive impacts of epigenetics and semen parameters, further investigations can provide useful information for better freezing.
Substances chimiques
Cryoprotective Agents
0
Lecithins
0
Histones
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e14570Subventions
Organisme : Tarbiat Modares University
Informations de copyright
© 2024 Wiley‐VCH GmbH. Published by John Wiley & Sons Ltd.
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