Bright Red Bioluminescence from Semisynthetic NanoLuc (sNLuc).
Journal
ACS chemical biology
ISSN: 1554-8937
Titre abrégé: ACS Chem Biol
Pays: United States
ID NLM: 101282906
Informations de publication
Date de publication:
08 May 2024
08 May 2024
Historique:
medline:
8
5
2024
pubmed:
8
5
2024
entrez:
8
5
2024
Statut:
aheadofprint
Résumé
Red-shifted bioluminescence is highly desirable for diagnostic and imaging applications. Herein, we report a semisynthetic NanoLuc (sNLuc) based on complementation of a split NLuc (LgBiT) with a synthetic peptide (SmBiT) functionalized with a fluorophore for BRET emission. We observed exceptional BRET ratios with diverse fluorophores, notably in the red (
Identifiants
pubmed: 38717306
doi: 10.1021/acschembio.4c00033
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM