Transfection in Primary Cultured Neuronal Cells.

Neurons / metabolism Animals Transfection / methods Cells, Cultured Rats Receptors, N-Methyl-D-Aspartate / metabolism Mice Primary Cell Culture / methods DNA, Complementary / genetics
Culture Lipofection NMDA Neuron Transfection

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2024
Historique:
medline: 10 5 2024
pubmed: 10 5 2024
entrez: 10 5 2024
Statut: ppublish

Résumé

Transfection allows the introduction of foreign nucleic acid into eukaryotic cells. It is an important tool in understanding the roles of NMDARs in neurons. Here we describe using lipofection-mediated transfection to introduce cDNA encoding NMDAR subunits into postmitotic rodent primary cortical neurons maintained in culture.

Identifiants

DOI: 10.1007/978-1-0716-3830-9_4 PMID: 38727902
pubmed: 38727902
doi: 10.1007/978-1-0716-3830-9_4
doi:

Substances chimiques

Receptors, N-Methyl-D-Aspartate 0
DNA, Complementary 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

47-54

Informations de copyright

© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Références

Karra D, Dahm R (2010) Transfection techniques for neuronal cells. J Neurosci 30:6171–6177. https://doi.org/10.1523/JNEUROSCI.0183-10.2010
doi: 10.1523/JNEUROSCI.0183-10.2010 pubmed: 20445041 pmcid: 6632737
Zeitelhofer M, Vessey JP, Xie Y, Tübing F, Thomas S, Kiebler M et al (2007) High-efficiency transfection of mammalian neurons via nucleofection. Nat Protoc 2:1692–1704. https://doi.org/10.1038/nprot.2007.226
doi: 10.1038/nprot.2007.226 pubmed: 17641634
Marwick KFM, Hansen KB, Skehel PA, Hardingham GE, Wyllie DJA (2019) Functional assessment of triheteromeric NMDA receptors containing a human variant associated with epilepsy. J Physiol 597:1691–1704. https://doi.org/10.1113/JP277292
doi: 10.1113/JP277292 pubmed: 30604514 pmcid: 6418762
Martel MA, Ryan TJ, Bell KFS, Fowler JH, McMahon A, Al-Mubarak B et al (2012) The subtype of GluN2 C-terminal domain determines the response to excitotoxic insults. Neuron 74:543–556. https://doi.org/10.1016/j.neuron.2012.03.021
doi: 10.1016/j.neuron.2012.03.021 pubmed: 22578505 pmcid: 3398391
Felgner PL, Gadek TR, Holm M, Roman R, Chan HW, Wenz M et al (1987) Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure. Proc Natl Acad Sci USA 84:7413–7417. https://doi.org/10.1073/pnas.84.21.7413
doi: 10.1073/pnas.84.21.7413 pubmed: 2823261 pmcid: 299306
Felgner PL, Ringold GM (1989) Cationic liposome-mediated transfection. Nature 337:387–388. https://doi.org/10.1038/337387a0
doi: 10.1038/337387a0 pubmed: 2463491
Bading H, Ginty DD, Greenberg ME (1993) Regulation of gene expression in hippocampal neurons by distinct calcium signaling pathways. Science 260:181–186. https://doi.org/10.1126/science.8097060
doi: 10.1126/science.8097060 pubmed: 8097060
Papadia S, Soriano FX, Léveillé F, Martel M-A, Dakin KA, Hansen HH et al (2008) Synaptic NMDA receptor activity boosts intrinsic antioxidant defenses. Nat Neurosci 11:476–487. https://doi.org/10.1038/nn2071
doi: 10.1038/nn2071 pubmed: 18344994 pmcid: 2556874
Butash KA, Natarajan P, Young A, Fox DK (2000) Reexamination of the effect of endotoxin on cell proliferation and transfection efficiency. BioTechniques 29:610–619. https://doi.org/10.2144/00293rr04
doi: 10.2144/00293rr04 pubmed: 10997275

Auteurs

Katie F M Marwick (KFM)

Centre for Discovery Brain Science, University of Edinburgh, Edinburgh, UK. Katie.Marwick@ed.ac.uk.

Giles E Hardingham (GE)

Centre for Discovery Brain Science, University of Edinburgh, Edinburgh, UK.

Articles similaires

Evaluating the efficacy of telesurgery with dual console SSI Mantra Surgical Robotic System: experiment on animal model and clinical trials.

Sudhir Prem Srivastava, Vishwajyoti Pascual Srivastava, Avinesh Singh et al.
1.00
Robotic Surgical Procedures Animals Humans Telemedicine Models, Animal

Odour generalisation and detection dog training.

Lyn Caldicott, Thomas W Pike, Helen E Zulch et al.
1.00
Animals Odorants Dogs Generalization, Psychological Smell

FBXO22 inhibits colitis and colorectal carcinogenesis by regulating the degradation of the S2448-phosphorylated form of mTOR.

Minle Li, Xuan Chen, Pengfei Qu et al.
1.00
Animals TOR Serine-Threonine Kinases Colorectal Neoplasms Colitis Mice

Use of organic material provided by an automatic enrichment device by weaner pigs and its influence on tail lesions.

Philipp Heseker, Jeanette Probst, Stefanie Ammer et al.
1.00
Animals Tail Swine Behavior, Animal Animal Husbandry

Classifications MeSH

questionsmedicales.fr › Cellules › Neurones › Transfection in Primary Cultured Neuronal Cells.
questionsmedicales.fr › Eucaryotes › Animaux › Transfection in Primary Cultured Neuronal Cells.
questionsmedicales.fr › Phénomènes génétiques › Recombinaison génétique › Transfection › Transfection in Primary Cultured Neuronal Cells.
questionsmedicales.fr › Cellules › Cellules cultivées › Transfection in Primary Cultured Neuronal Cells.
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Rats › Transfection in Primary Cultured Neuronal Cells.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Récepteurs de surface cellulaire › Récepteurs aux neuromédiateurs › Récepteurs aux acides aminés › Récepteurs au glutamate › Récepteurs ionotropes du glutamate › Récepteurs du N-méthyl-D-aspartate › Transfection in Primary Cultured Neuronal Cells.
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Transfection in Primary Cultured Neuronal Cells.
questionsmedicales.fr › Techniques d'investigation › Techniques in vitro › Techniques de culture › Techniques de culture cellulaire › Culture de cellules primaires › Transfection in Primary Cultured Neuronal Cells.
questionsmedicales.fr › Actions chimiques et utilisations › Utilisations spécialisées de produits chimiques › Produits chimiques de laboratoire › Sondes moléculaires › Sondes d'acide nucléique › Sondes d'ADN › ADN complémentaire › Transfection in Primary Cultured Neuronal Cells.