Effects of ketamine on penile tissues in an experimental priapism model in rats.
Ratlarda deneysel priapizm modelinde ketamin'in penil dokular üzerine etkileri.
Animals
Ketamine
/ administration & dosage
Male
Priapism
/ drug therapy
Rats
Penis
/ drug effects
Reperfusion Injury
/ drug therapy
Disease Models, Animal
Malondialdehyde
/ metabolism
Tumor Necrosis Factor-alpha
/ blood
Random Allocation
Anesthetics, Dissociative
/ administration & dosage
Interleukin-1beta
/ metabolism
Journal
Ulusal travma ve acil cerrahi dergisi = Turkish journal of trauma & emergency surgery : TJTES
ISSN: 1307-7945
Titre abrégé: Ulus Travma Acil Cerrahi Derg
Pays: Turkey
ID NLM: 101274231
Informations de publication
Date de publication:
May 2024
May 2024
Historique:
medline:
13
5
2024
pubmed:
13
5
2024
entrez:
13
5
2024
Statut:
ppublish
Résumé
This study aimed to evaluate the histopathological and biochemical effects of ketamine on penile tissues following ischemia-reperfusion injury induced by priapism. Twenty-four male rats were randomized into three groups. Group 1 served as the control group. Group 2 underwent the priapism model to induce ischemia-reperfusion injury. Group 3, the treatment group, experienced a similar ischemia-reperfusion model as Group 2; additionally, 50 mg/kg of ketamine was administered intraperitoneally just before reperfusion. Blood biochemical analyses and penile histopathological evaluations were performed. In Group 3, significant improvements were observed in all histopathological scores, including desquamation, edema, inflammation, and vasocongestion compared to Group 2 (p<0.001). Blood biochemical analyses showed that the malondialdehyde (MDA) levels were recorded as 10 in Group 2, with a significant decrease in Group 3 (p=0.013). Similarly, proinflammatory cytokine levels, including interleukin-1 beta (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-α), were found to be suppressed in Group 3 compared to Group 2 (p=0.003, p=0.022, and p=0.028, respectively). Antioxidant enzyme activities, such as glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD), were higher in Group 3 compared to Group 2 (p=0.016 and p=0.024, respec-tively). Ketamine is an effective anesthetic agent in alleviating the effects of penile ischemia-reperfusion injury.
Sections du résumé
BACKGROUND
BACKGROUND
This study aimed to evaluate the histopathological and biochemical effects of ketamine on penile tissues following ischemia-reperfusion injury induced by priapism.
METHODS
METHODS
Twenty-four male rats were randomized into three groups. Group 1 served as the control group. Group 2 underwent the priapism model to induce ischemia-reperfusion injury. Group 3, the treatment group, experienced a similar ischemia-reperfusion model as Group 2; additionally, 50 mg/kg of ketamine was administered intraperitoneally just before reperfusion. Blood biochemical analyses and penile histopathological evaluations were performed.
RESULTS
RESULTS
In Group 3, significant improvements were observed in all histopathological scores, including desquamation, edema, inflammation, and vasocongestion compared to Group 2 (p<0.001). Blood biochemical analyses showed that the malondialdehyde (MDA) levels were recorded as 10 in Group 2, with a significant decrease in Group 3 (p=0.013). Similarly, proinflammatory cytokine levels, including interleukin-1 beta (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-α), were found to be suppressed in Group 3 compared to Group 2 (p=0.003, p=0.022, and p=0.028, respectively). Antioxidant enzyme activities, such as glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD), were higher in Group 3 compared to Group 2 (p=0.016 and p=0.024, respec-tively).
CONCLUSION
CONCLUSIONS
Ketamine is an effective anesthetic agent in alleviating the effects of penile ischemia-reperfusion injury.
Identifiants
pubmed: 38738674
doi: 10.14744/tjtes.2024.33262
doi:
Substances chimiques
Ketamine
690G0D6V8H
Malondialdehyde
4Y8F71G49Q
Tumor Necrosis Factor-alpha
0
Anesthetics, Dissociative
0
Interleukin-1beta
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM