Applying Reverse Genetics to Study Measles Virus Interactions with the Host.
Affinity purification
Measles virus
Recombinant virus
Reverse genetics
Tagged proteins
Virus-host interactions
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2024
2024
Historique:
medline:
15
5
2024
pubmed:
15
5
2024
entrez:
14
5
2024
Statut:
ppublish
Résumé
The study of virus-host interactions is essential to achieve a comprehensive understanding of the viral replication process. The commonly used methods are yeast two-hybrid approach and transient expression of a single tagged viral protein in host cells followed by affinity purification of interacting cellular proteins and mass spectrometry analysis (AP-MS). However, by these approaches, virus-host protein-protein interactions are detected in the absence of a real infection, not always correctly compartmentalized, and for the yeast two-hybrid approach performed in a heterologous system. Thus, some of the detected protein-protein interactions may be artificial. Here we describe a new strategy based on recombinant viruses expressing tagged viral proteins to capture both direct and indirect protein partners during the infection (AP-MS in viral context). This way, virus-host protein-protein interacting co-complexes can be purified directly from infected cells for further characterization.
Identifiants
pubmed: 38743364
doi: 10.1007/978-1-0716-3870-5_7
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
89-103Informations de copyright
© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
Références
Fodor E, Devenish L, Engelhardt OG, Palese P, Brownlee GG, Garcia-Sastre A (1999) Rescue of influenza A virus from recombinant DNA. J Virol 73(11):9679–9682. https://doi.org/10.1128/jvi.73.11.9679-9682.1999
doi: 10.1128/jvi.73.11.9679-9682.1999
pubmed: 10516084
pmcid: 113010
Nakaya T, Cros J, Park MS, Nakaya Y, Zheng HY, Sagrera A, Villar E, Garcia-Sastre A, Palese P (2001) Recombinant Newcastle disease virus as a vaccine vector. J Virol 75(23):11868–11873. https://doi.org/10.1128/jvi.75.23.11868-11873.2001
doi: 10.1128/jvi.75.23.11868-11873.2001
pubmed: 11689668
pmcid: 114773
Yun SI, Kim SY, Rice CM, Lee YM (2003) Development and application of a reverse genetics system for Japanese encephalitis virus. J Virol 77(11):6450–6465. https://doi.org/10.1128/jvi.77.11.6450-6465.2003
doi: 10.1128/jvi.77.11.6450-6465.2003
pubmed: 12743302
pmcid: 154991
Billeter MA, Naim HY, Udem SA (2009) Reverse genetics of measles virus and resulting multivalent recombinant vaccines: applications of recombinant measles viruses. Curr Top Microbiol Immunol 329:129–162. https://doi.org/10.1007/978-3-540-70523-9_7
doi: 10.1007/978-3-540-70523-9_7
pubmed: 19198565
pmcid: 7120638
Isel C, Munier S, Naffakh N (2016) Experimental approaches to study genome packaging of influenza A viruses. Viruses 8(8):218. https://doi.org/10.3390/v8080218
doi: 10.3390/v8080218
pubmed: 27517951
pmcid: 4997580
Neumann G, Kawaoka Y (2001) Reverse genetics of influenza virus. Virology 287(2):243–250. https://doi.org/10.1006/viro.2001.1008
doi: 10.1006/viro.2001.1008
pubmed: 11531402
Stobart CC, Moore ML (2014) RNA virus reverse genetics and vaccine design. Viruses 6(7):2531–2550. https://doi.org/10.3390/v6072531
doi: 10.3390/v6072531
pubmed: 24967693
pmcid: 4113782
Radecke F, Spielhofer P, Schneider H, Kaelin K, Huber M, Dotsch C, Christiansen G, Billeter MA (1995) Rescue of measles viruses from cloned DNA. EMBO J 14(23):5773–5784. https://doi.org/10.1002/j.1460-2075.1995.tb00266.x
doi: 10.1002/j.1460-2075.1995.tb00266.x
pubmed: 8846771
pmcid: 394696
Combredet C, Labrousse V, Mollet L, Lorin C, Delebecque F, Hurtrel B, McClure H, Feinberg MB, Brahic M, Tangy F (2003) A molecularly cloned Schwarz strain of measles virus vaccine induces strong immune responses in macaques and transgenic mice. J Virol 77(21):11546–11554. https://doi.org/10.1128/jvi.77.21.11546-11554.2003
doi: 10.1128/jvi.77.21.11546-11554.2003
pubmed: 14557640
pmcid: 229349
Mahmoudi Gomari M, Saraygord-Afshari N, Farsimadan M, Rostami N, Aghamiri S, Farajollahi MM (2020) Opportunities and challenges of the tag-assisted protein purification techniques: applications in the pharmaceutical industry. Biotechnol Adv 45:107653. https://doi.org/10.1016/j.biotechadv.2020.107653
doi: 10.1016/j.biotechadv.2020.107653
pubmed: 33157154
Ohashi M, Holthaus AM, Calderwood MA, Lai C-Y, Krastins B, Sarracino D, Johannsen E (2015) The EBNA3 family of Epstein-Barr virus nuclear proteins associates with the USP46/USP12 deubiquitination complexes to regulate lymphoblastoid cell line growth. PLoS Pathog 11(4):e1004822. https://doi.org/10.1371/journal.ppat.1004822
doi: 10.1371/journal.ppat.1004822
pubmed: 25855980
pmcid: 4391933
Fan J, Xiao P, Kong D, Liu X, Meng L, An T, Cai X, Wang H, Yu L (2022) Engineering His-tagged Senecavirus A for one-step purification of viral antigens. Vaccines (Basel) 10(2):170. https://doi.org/10.3390/vaccines10020170
doi: 10.3390/vaccines10020170
pubmed: 35214628
Ma X, Li C, Xia Q, Zhang Y, Yang Y, Wahaab A, Liu K, Li Z, Li B, Qiu Y, Wei J, Ma Z (2022) Construction of a recombinant Japanese encephalitis virus with a hemagglutinin-tagged NS2A: a model for an analysis of biological characteristics and functions of NS2A during viral infection. Viruses 14(4):706. https://doi.org/10.3390/v14040706
doi: 10.3390/v14040706
pubmed: 35458436
pmcid: 9024733
Skerra A, Schmidt TGM (1999) Applications of a peptide ligand for streptavidin: the Strep-tag. Biomol Eng 16(1–4):79–86. https://doi.org/10.1016/s1050-3862(99)00033-9
doi: 10.1016/s1050-3862(99)00033-9
pubmed: 10796988
Mayer D, Molawi K, Martinez-Sobrido L, Ghanem A, Thomas S, Baginsky S, Grossmann J, Garcia-Sastre A, Schwemmle M (2007) Identification of cellular interaction partners of the influenza virus ribonucleoprotein complex and polymerase complex using proteomic-based approaches. J Proteome Res 6(2):672–682. https://doi.org/10.1021/pr060432u
doi: 10.1021/pr060432u
pubmed: 17269724
pmcid: 2577182
De Maio FA, Risso G, Iglesias NG, Shah P, Pozzi B, Gebhard LG, Mammi P, Mancini E, Yanovsky MJ, Andino R, Krogan N, Srebrow A, Gamarnik AV (2016) The dengue virus NS5 protein intrudes in the cellular spliceosome and modulates splicing. PLoS Pathog 12(8):e1005841. https://doi.org/10.1371/journal.ppat.1005841
doi: 10.1371/journal.ppat.1005841
pubmed: 27575636
pmcid: 5004807
Fournier G, Chiang C, Munier S, Tomoiu A, Demeret C, Vidalain PO, Jacob Y, Naffakh N (2014) Recruitment of RED-SMU1 complex by Influenza A virus RNA polymerase to control viral mRNA splicing. PLoS Pathog 10(6):e1004164. https://doi.org/10.1371/journal.ppat.1004164
doi: 10.1371/journal.ppat.1004164
pubmed: 24945353
pmcid: 4055741
Li Y, Frederick KM, Haverland NA, Ciborowski P, Belshan M (2016) Investigation of the HIV-1 matrix interactome during virus replication. Proteomics Clin Appl 10(2):156–163. https://doi.org/10.1002/prca.201400189
doi: 10.1002/prca.201400189
pubmed: 26360636
Komarova AV, Combredet C, Meyniel-Schicklin L, Chapelle M, Caignard G, Camadro JM, Lotteau V, Vidalain PO, Tangy F (2011) Proteomic analysis of virus-host interactions in an infectious context using recombinant viruses. Mol Cell Proteomics 10(12):M110.007443. https://doi.org/10.1074/mcp.M110.007443
doi: 10.1074/mcp.M110.007443
pubmed: 21911578
pmcid: 3237069
Meignie A, Combredet C, Santolini M, Kovacs IA, Douche T, Gianetto QG, Eun H, Matondo M, Jacob Y, Grailhe R, Tangy F, Komarova AV (2021) Proteomic analysis uncovers measles virus protein C interaction with p65-iASPP protein complex. Mol Cell Proteomics 20:100049. https://doi.org/10.1016/j.mcpro.2021.100049
doi: 10.1016/j.mcpro.2021.100049
pubmed: 33515806
pmcid: 7950213
Komarova AV, Combredet C, Sismeiro O, Dillies MA, Jagla B, Sanchez David RY, Vabret N, Coppee JY, Vidalain PO, Tangy F (2013) Identification of RNA partners of viral proteins in infected cells. RNA Biol 10(6):944–956. https://doi.org/10.4161/rna.24453
doi: 10.4161/rna.24453
pubmed: 23595062
Iverson LE, Rose JK (1981) Localized attenuation and discontinuous synthesis during vesicular stomatitis virus transcription. Cell 23(2):477–484. https://doi.org/10.1016/0092-8674(81)90143-4
doi: 10.1016/0092-8674(81)90143-4
pubmed: 6258804
Muhlberger E (2007) Filovirus replication and transcription. Future Virol 2(2):205–215. https://doi.org/10.2217/17460794.2.2.205
doi: 10.2217/17460794.2.2.205
pubmed: 24093048
pmcid: 3787895
Cattaneo R, Rebmann G, Schmid A, Baczko K, ter Meulen V, Billeter MA (1987) Altered transcription of a defective measles virus genome derived from a diseased human brain. EMBO J 6(3):681–688. https://doi.org/10.1002/j.1460-2075.1987.tb04808.x
doi: 10.1002/j.1460-2075.1987.tb04808.x
pubmed: 3582370
pmcid: 553451
Plumet S, Duprex WP, Gerlier D (2005) Dynamics of viral RNA synthesis during measles virus infection. J Virol 79(11):6900–6908. https://doi.org/10.1128/JVI.79.11.6900-6908.2005
doi: 10.1128/JVI.79.11.6900-6908.2005
pubmed: 15890929
pmcid: 1112129