Preservation of Bacillus subtilis' cellular liquid state at deep sub-zero temperatures in perchlorate brines.
Journal
Communications biology
ISSN: 2399-3642
Titre abrégé: Commun Biol
Pays: England
ID NLM: 101719179
Informations de publication
Date de publication:
16 May 2024
16 May 2024
Historique:
received:
16
11
2023
accepted:
02
05
2024
medline:
17
5
2024
pubmed:
17
5
2024
entrez:
16
5
2024
Statut:
epublish
Résumé
Although a low temperature limit for life has not been established, it is thought that there exists a physical limit imposed by the onset of intracellular vitrification, typically occurring at ~-20 °C for unicellular organisms. Here, we show, through differential scanning calorimetry, that molar concentrations of magnesium perchlorate can depress the intracellular vitrification point of Bacillus subtilis cells to temperatures much lower than those previously reported. At 2.5 M Mg(ClO
Identifiants
pubmed: 38755264
doi: 10.1038/s42003-024-06277-4
pii: 10.1038/s42003-024-06277-4
doi:
Substances chimiques
perchlorate
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
588Subventions
Organisme : RCUK | Science and Technology Facilities Council (STFC)
ID : ST/V000586/1
Informations de copyright
© 2024. The Author(s).
Références
Clarke, A. The thermal limits to life on Earth. Int. J. Astrobiol. 13, 141–154 (2014).
doi: 10.1017/S1473550413000438
Price, P. B. & Sowers, T. Temperature dependence of metabolic rates for microbial growth, maintenance, and survival. Proc. Natl Acad. Sci. 101, 4631–4636 (2004).
doi: 10.1073/pnas.0400522101
pubmed: 15070769
pmcid: 384798
Clarke, A. et al. A Low Temperature Limit for Life on Earth. PLoS One 8, e66207 (2013).
doi: 10.1371/journal.pone.0066207
pubmed: 23840425
pmcid: 3686811
Debenedetti, P. G. Metastable Liquids: Concepts and Principles. 1 (Princeton University Press, 1996).
Mykytczuk, N. C. S. et al. Bacterial growth at −15 °C; molecular insights from the permafrost bacterium Planococcus halocryophilus Or1. ISME J. 7, 1211–1226 (2013).
doi: 10.1038/ismej.2013.8
pubmed: 23389107
pmcid: 3660685
Sformo, T. et al. Deep supercooling, vitrification and limited survival to –100 °C in the Alaskan beetle Cucujus clavipes puniceus (Coleoptera: Cucujidae) larvae. J. Exp. Biol. 213, 502–509 (2010).
doi: 10.1242/jeb.035758
pubmed: 20086136
Fonseca, F., Meneghel, J., Cenard, S., Passot, S. & Morris, G. J. Determination of Intracellular Vitrification Temperatures for Unicellular Micro Organisms under Conditions Relevant for Cryopreservation. PLoS One 11, e0152939 (2016).
doi: 10.1371/journal.pone.0152939
pubmed: 27055246
pmcid: 4824440
Sharma, S. et al. Diverse organic-mineral associations in Jezero crater, Mars. Nature 619, 724–732 (2023).
doi: 10.1038/s41586-023-06143-z
pubmed: 37438522
pmcid: 10371864
Clifford, S. M. et al. Depth of the Martian cryosphere: Revised estimates and implications for the existence and detection of subpermafrost groundwater. J. Geophys. Res. 115, E07001 (2010).
Orosei, R. et al. Radar evidence of subglacial liquid water on Mars. Science 361, 490–493 (2018).
doi: 10.1126/science.aar7268
pubmed: 30045881
Lauro, S. E. et al. Multiple subglacial water bodies below the south pole of Mars unveiled by new MARSIS data. Nat. Astron. 5, 63–70 (2021).
doi: 10.1038/s41550-020-1200-6
Michalski, J. R. et al. The Martian subsurface as a potential window into the origin of life. Nat. Geosci. 11, 21–26 (2018).
doi: 10.1038/s41561-017-0015-2
Khurana, K. K. et al. Induced magnetic fields as evidence for subsurface oceans in Europa and Callisto. Nature 395, 777–780 (1998).
doi: 10.1038/27394
pubmed: 9796812
Kivelson, M. G. et al. Galileo Magnetometer Measurements: A Stronger Case for a Subsurface Ocean at Europa. Science 289, 1340–1343 (2000).
doi: 10.1126/science.289.5483.1340
pubmed: 10958778
Lorenz, R. D. et al. Titan’s Rotation Reveals an Internal Ocean and Changing Zonal Winds. Science 319, 1649–1651 (2008).
doi: 10.1126/science.1151639
pubmed: 18356521
Thomas, P. C. et al. Enceladus’s measured physical libration requires a global subsurface ocean. Icarus 264, 37–47 (2016).
doi: 10.1016/j.icarus.2015.08.037
Bravenec, A. D. & Catling, D. C. Effect of Concentration, Cooling, and Warming Rates on Glass Transition Temperatures for NaClO
doi: 10.1021/acsearthspacechem.3c00090
Shumway, A. O., Catling, D. C. & Toner, J. D. Regolith Inhibits Salt and Ice Crystallization in Mg(ClO
doi: 10.3847/PSJ/ace891
Hecht, M. H. et al. Detection of perchlorate and the soluble chemistry of martian soil at the phoenix lander site. Science 325, 64–67 (2009).
doi: 10.1126/science.1172466
pubmed: 19574385
Gault, S., Jaworek, M. W., Winter, R. & Cockell, C. S. High pressures increase α-chymotrypsin enzyme activity under perchlorate stress. Commun. Biol. 3, 550 (2020).
doi: 10.1038/s42003-020-01279-4
pubmed: 33009512
pmcid: 7532203
Gault, S., Jaworek, M. W., Winter, R. & Cockell, C. S. Perchlorate salts confer psychrophilic characteristics in α-chymotrypsin. Sci. Rep. 11, 16523 (2021).
doi: 10.1038/s41598-021-95997-2
pubmed: 34400699
pmcid: 8367967
Stevens, A. H. & Cockell, C. S. A Systematic Study of the Limits of Life in Mixed Ion Solutions: Physicochemical Parameters Do Not Predict Habitability. Front. Microbiol. 11, 1478 (2020).
Kriegler, S. et al. Structural responses of model biomembranes to Mars-relevant salts. Phys. Chem. Chem. Phys. 23, 14212–14223 (2021).
doi: 10.1039/D1CP02092G
pubmed: 34159996
Tortorella, A., Oliva, R., Giancola, C., Petraccone, L. & Winter, R. Bacterial model membranes under the harsh subsurface conditions of Mars. Phys. Chem. Chem. Phys. 26, 760–769 (2023).
Paula, S., Volkov, A. G. & Deamer, D. W. Permeation of Halide Anions through Phospholipid Bilayers Occurs by the Solubility-Diffusion Mechanism. Biophys. J. 74, 319–327 (1998).
doi: 10.1016/S0006-3495(98)77789-6
pubmed: 9449332
pmcid: 1299384
Miles, C. A. Relating Cell Killing to Inactivation of Critical Components. Appl. Environ. Microbiol. 72, 914–917 (2006).
doi: 10.1128/AEM.72.1.914-917.2006
pubmed: 16391134
pmcid: 1352265
Knop, J.-M. et al. Life in Multi-Extreme Environments: Brines, Osmotic and Hydrostatic Pressure─A Physicochemical View. Chem. Rev. 123, 73–104 (2023).
doi: 10.1021/acs.chemrev.2c00491
pubmed: 36260784
Privalov, P. L. Cold denaturation of proteins. Crit. Rev. Biochem. Mol. Biol. 25, 281–305 (1990).
doi: 10.3109/10409239009090612
pubmed: 2225910
Pastore, A. et al. Unbiased Cold Denaturation: Low- and High-Temperature Unfolding of Yeast Frataxin under Physiological Conditions. J. Am. Chem. Soc. 129, 5374–5375 (2007).
doi: 10.1021/ja0714538
pubmed: 17411056
pmcid: 2664662
Georlette, D. et al. Some like it cold: biocatalysis at low temperatures. FEMS Microbiol. Rev. 28, 25–42 (2004).
doi: 10.1016/j.femsre.2003.07.003
pubmed: 14975528
D’Amico, S., Collins, T., Marx, J.-C., Feller, G. & Gerday, C. Psychrophilic microorganisms: challenges for life. EMBO Rep. 7, 385–389 (2006).
doi: 10.1038/sj.embor.7400662
pubmed: 16585939
pmcid: 1456908
Phadtare, S. Recent Developments in Bacterial Cold-Shock Response. Curr. Issues Mol. Biol. 6, 125–136 (2004).
pubmed: 15119823
Okur, H. I. et al. Beyond the Hofmeister Series: Ion-Specific Effects on Proteins and Their Biological Functions. J. Phys. Chem. B 121, 1997–2014 (2017).
doi: 10.1021/acs.jpcb.6b10797
pubmed: 28094985
Gault, S. & Cockell, C. S. Perchlorate Salts Exert a Dominant, Deleterious Effect on the Structure, Stability, and Activity of α-Chymotrypsin. Astrobiology 21, 405–412 (2021).
doi: 10.1089/ast.2020.2223
pubmed: 33784200
Džupponová, V., Tomášková, N., Antošová, A., Sedlák, E. & Žoldák, G. Salt-Specific Suppression of the Cold Denaturation of Thermophilic Multidomain Initiation Factor 2. Int. J. Mol. Sci. 24, 6787 (2023).
Gautier, J. et al. A low membrane lipid phase transition temperature is associated with a high cryotolerance of Lactobacillus delbrueckii subspecies bulgaricus CFL1. J. Dairy Sci. 96, 5591–5602 (2013).
doi: 10.3168/jds.2013-6802
pubmed: 23810590