Measuring Replicative Lifespan in Cryptococcus neoformans.
Aging
Longevity
Microdissection
Microfluidic system
Yeast
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2024
2024
Historique:
medline:
17
5
2024
pubmed:
17
5
2024
entrez:
17
5
2024
Statut:
ppublish
Résumé
Advances in understanding cellular aging research have been possible due to the analysis of the replicative lifespan of yeast cells. Studying longevity in the pathogenic yeast Cryptococcus neoformans is essential because old yeast cells with age-related phenotypes accumulate during infection and are associated with increased virulence and antifungal tolerance. Microdissection and microfluidic devices are valuable tools for continuously tracking cells at the single-cell level. In this chapter, we describe the features of these two platforms and outline technical limitations and information to study aging mechanisms while assessing the lifespan of yeast cells.
Identifiants
pubmed: 38758331
doi: 10.1007/978-1-0716-3722-7_25
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
375-384Informations de copyright
© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
Références
Spivey EC, Jones SK Jr, Rybarski JR et al (2017) An aging-independent replicative lifespan in a symmetrically dividing eukaryote. eLife 6:e20340. https://doi.org/10.7554/eLife.20340
doi: 10.7554/eLife.20340
pubmed: 28139976
pmcid: 5332158
Silva VKA, Bhattacharya S, Oliveira NK et al (2022) Replicative aging remodels the cell wall and is associated with increased intracellular trafficking in human pathogenic yeasts. MBio 13(1):e00190–e00122. https://doi.org/10.1128/mbio.00190-22
doi: 10.1128/mbio.00190-22
pmcid: 8844920
Jain N, Cook E, Xess I et al (2009) Isolation and characterization of senescent Cryptococcus neoformans and implications for phenotypic switching and pathogenesis in chronic cryptococcosis. Eukaryot Cell 8(6):858–866. https://doi.org/10.1128/EC.00017-09
doi: 10.1128/EC.00017-09
pubmed: 19411622
pmcid: 2698302
Coody TK, Hughes AL (2018) Advancing the aging biology toolkit. eLife 7:e42976. https://doi.org/10.7554/eLife.42976
doi: 10.7554/eLife.42976
pubmed: 30484772
pmcid: 6261250
Bouklas T, Alonso-Crisóstomo L, Székely T Jr et al (2017) Generational distribution of a Candida glabrata population: resilient old cells prevail, while younger cells dominate in the vulnerable host. PLoS Pathog 13(5):e1006355–e1006355. https://doi.org/10.1371/journal.ppat.1006355
doi: 10.1371/journal.ppat.1006355
pubmed: 28489916
pmcid: 5440053
Orner EP, Zhang P, Jo MC et al (2019) High-throughput yeast aging analysis for Cryptococcus (HYAAC) microfluidic device streamlines aging studies in Cryptococcus neoformans. Commun Biol 2(1):256. https://doi.org/10.1038/s42003-019-0504-5
doi: 10.1038/s42003-019-0504-5
pubmed: 31312725
pmcid: 6620289
Park PU, McVey M, Guarente L (2002) Separation of mother and daughter cells. In: Methods in enzymology, vol 351. Academic Press, pp 468–477. https://doi.org/10.1016/S0076-6879(02)51865-6
doi: 10.1016/S0076-6879(02)51865-6
Lee SS, Vizcarra IA, Huberts DHEW et al (2012) Whole lifespan microscopic observation of budding yeast aging through a microfluidic dissection platform. Proc Natl Acad Sci 109(13):4916–4920. https://doi.org/10.1073/pnas.1113505109
doi: 10.1073/pnas.1113505109
pubmed: 22421136
pmcid: 3324001
Huberts DHEW, González J, Lee SS et al (2014) Calorie restriction does not elicit a robust extension of replicative lifespan in Saccharomyces cerevisiae. Proc Natl Acad Sci 111(32):11727–11731. https://doi.org/10.1073/pnas.1410024111
doi: 10.1073/pnas.1410024111
pubmed: 25071164
pmcid: 4136557
Jo MC, Liu W, Gu L et al (2015) High-throughput analysis of yeast replicative aging using a microfluidic system. Proc Natl Acad Sci 112(30):9364–9369. https://doi.org/10.1073/pnas.1510328112
doi: 10.1073/pnas.1510328112
pubmed: 26170317
pmcid: 4522780
Crane MM, Clark IBN, Bakker E et al (2014) A microfluidic system for studying ageing and dynamic single-cell responses in budding yeast. PLoS One 9(6):e100042. https://doi.org/10.1371/journal.pone.0100042
doi: 10.1371/journal.pone.0100042
pubmed: 24950344
pmcid: 4065030