Lipopolysaccharide with long O-antigen is crucial for Salmonella Enteritidis to evade complement activity and to facilitate bacterial survival in vivo in the Galleria mellonella infection model.
Salmonella enteritidis
/ immunology
Animals
O Antigens
/ immunology
Complement System Proteins
/ immunology
Disease Models, Animal
Lipopolysaccharides
/ immunology
Immune Evasion
Microbial Viability
Moths
/ microbiology
Virulence
Salmonella Infections
/ immunology
Salmonella Infections, Animal
/ immunology
Complement Activation
Lepidoptera
/ immunology
Galleria mellonella
Salmonella Enteritidis
Complement
Long O-antigen
O-antigen
Very long O-antigen
Journal
Medical microbiology and immunology
ISSN: 1432-1831
Titre abrégé: Med Microbiol Immunol
Pays: Germany
ID NLM: 0314524
Informations de publication
Date de publication:
20 May 2024
20 May 2024
Historique:
received:
28
11
2023
accepted:
19
04
2024
medline:
20
5
2024
pubmed:
20
5
2024
entrez:
20
5
2024
Statut:
epublish
Résumé
Bacterial resistance to serum is a key virulence factor for the development of systemic infections. The amount of lipopolysaccharide (LPS) and the O-antigen chain length distribution on the outer membrane, predispose Salmonella to escape complement-mediated killing. In Salmonella enterica serovar Enteritidis (S. Enteritidis) a modal distribution of the LPS O-antigen length can be observed. It is characterized by the presence of distinct fractions: low molecular weight LPS, long LPS and very long LPS. In the present work, we investigated the effect of the O-antigen modal length composition of LPS molecules on the surface of S. Enteritidis cells on its ability to evade host complement responses. Therefore, we examined systematically, by using specific deletion mutants, roles of different O-antigen fractions in complement evasion. We developed a method to analyze the average LPS lengths and investigated the interaction of the bacteria and isolated LPS molecules with complement components. Additionally, we assessed the aspect of LPS O-antigen chain length distribution in S. Enteritidis virulence in vivo in the Galleria mellonella infection model. The obtained results of the measurements of the average LPS length confirmed that the method is suitable for measuring the average LPS length in bacterial cells as well as isolated LPS molecules and allows the comparison between strains. In contrast to earlier studies we have used much more precise methodology to assess the LPS molecules average length and modal distribution, also conducted more subtle analysis of complement system activation by lipopolysaccharides of various molecular mass. Data obtained in the complement activation assays clearly demonstrated that S. Enteritidis bacteria require LPS with long O-antigen to resist the complement system and to survive in the G. mellonella infection model.
Identifiants
pubmed: 38767707
doi: 10.1007/s00430-024-00790-3
pii: 10.1007/s00430-024-00790-3
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
8Subventions
Organisme : National Science Center Poland
ID : 2017/25/N/NZ6/02295
Organisme : The Polish National Agency for Academic Exchange
ID : PPN/IWA/2018/1/00034/U/00001
Organisme : The Sigrid Juselius Foundation
ID : 4708373
Organisme : Helsinki University Hospital
ID : TYH2022315
Informations de copyright
© 2024. The Author(s).
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