Control of artificial membrane fusion in physiological ionic solutions beyond the limits of electroformation.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
28 May 2024
28 May 2024
Historique:
received:
26
09
2023
accepted:
15
05
2024
medline:
29
5
2024
pubmed:
29
5
2024
entrez:
28
5
2024
Statut:
epublish
Résumé
Membrane fusion, merging two lipid bilayers, is crucial for fabricating artificial membrane structures. Over the past 40 years, in contrast to precise and controllable membrane fusion in-vivo through specific molecules such as SNAREs, controlling the fusion in-vitro while fabricating artificial membrane structures in physiological ionic solutions without fusion proteins has been a challenge, becoming a significant obstacle to practical applications. We present an approach consisting of an electric field and a few kPa hydraulic pressure as an additional variable to physically control the fusion, enabling tuning of the shape and size of the 3D freestanding lipid bilayers in physiological ionic solutions. Mechanical model analysis reveals that pressure-induced parallel/normal tensions enhance fusion among membranes in the microwell. In-vitro peptide-membrane assay, mimicking vesicular transport via pressure-assisted fusion, and stability of 38 days with in-chip pressure control via pore size-regulated hydrogel highlight the potential for diverse biological applications.
Identifiants
pubmed: 38806492
doi: 10.1038/s41467-024-48875-0
pii: 10.1038/s41467-024-48875-0
doi:
Substances chimiques
Lipid Bilayers
0
Ions
0
Membranes, Artificial
0
Hydrogels
0
Peptides
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
4524Subventions
Organisme : National Research Foundation of Korea (NRF)
ID : NRF-2020R1A2C2100363
Informations de copyright
© 2024. The Author(s).
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