A three-dimensional cell culture approach to investigate cytotoxic effects and production of inflammatory mediators by epoxy resin-based and calcium silicate-based endodontic sealer.
Root Canal Filling Materials
/ pharmacology
Epoxy Resins
Humans
Calcium Compounds
/ pharmacology
Silicates
/ pharmacology
Cell Survival
/ drug effects
Materials Testing
Cell Culture Techniques, Three Dimensional
/ methods
Inflammation Mediators
/ metabolism
Microscopy, Fluorescence
Osteoblasts
/ drug effects
Calcium silicate-based
Cell viability assays
Epoxy resin-based
Inflammatory mediators
Three-Dimensional Cell Culture
Journal
Clinical oral investigations
ISSN: 1436-3771
Titre abrégé: Clin Oral Investig
Pays: Germany
ID NLM: 9707115
Informations de publication
Date de publication:
29 May 2024
29 May 2024
Historique:
received:
28
02
2024
accepted:
24
05
2024
medline:
29
5
2024
pubmed:
29
5
2024
entrez:
29
5
2024
Statut:
epublish
Résumé
The aim of the present study was to assess the cytocompatibility of epoxy resin-based AH Plus Jet (Dentsply De Trey, Konstanz, Germany), Sealer Plus (MK Life, Porto Alegre, Brazil), calcium silicate-based Bio-C Sealer (Angelus, Londrina, PR, Brazil), Sealer Plus BC (MK Life) and AH Plus BC (Dentsply) through a tridimensional (3D) culture model of human osteoblast-like cells. Spheroids of MG-63 cells were produced and exposed to fresh root canal sealers extracts by 24 h, and the cytotoxicity was assessed by the Lactate Dehydrogenase assay (LDH). The distribution of dead cells within the microtissue was assessed by fluorescence microscopy, and morphological effects were investigated by histological analysis. The secreted inflammatory mediators were detected in cell supernatants through flow luminometry (XMap Luminex). Cells incubated with AH Plus Jet, AH Plus BC, Sealer Plus BC and Bio-C Sealer extracts showed high rates of cell viability, while the Sealer Plus induced a significant reduction of cell viability, causing reduction on the spheroid structure. Sealer Plus and Seaker Plus BC caused alterations on 3D microtissue morphology. The AH Plus BC extract was associated with the downregulation of secretion of pro-inflammatory cytokines IL-5, IL-7, IP-10 and RANTES. The new AH Plus BC calcium silicate-based endodontic sealer did not reduce cell viability in vitro, while led to the downregulation of pro-inflammatory cytokines. Choosing the appropriate endodontic sealer is a crucial step. AH Plus BC demonstrated high cell viability and downregulation of pro-inflammatory cytokines, appearing reliable for clinical use, while Sealer Plus presented lower cytocompatibility.
Identifiants
pubmed: 38809444
doi: 10.1007/s00784-024-05743-x
pii: 10.1007/s00784-024-05743-x
doi:
Substances chimiques
Root Canal Filling Materials
0
Epoxy Resins
0
Calcium Compounds
0
Silicates
0
calcium silicate
S4255P4G5M
epoxy resin-based root canal sealer
0
Inflammation Mediators
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
344Informations de copyright
© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
Références
Nair PN (2006) On the causes of persistent apical periodontitis: a review. Int Endod J. 2006 39(4):249 – 81. https://doi.org/10.1111/j.1365-2591.2006.01099.x
Pinheiro BC, Bramante AS, Hussne RP (2003) Influence of the penetration of instruments, used in the active lateral condensation, in the quality of root canal filling. J Appl Oral Sci 11(3):186–191. https://doi.org/10.1590/s1678-77572003000300006
doi: 10.1590/s1678-77572003000300006
pubmed: 21394391
Komabayashi T, Colmenar D, Cvach N, Bhat A, Primus C, Imai Y (2020) Comprehensive review of current endodontic sealers. Dent Mater J 29(5):703–720. https://doi.org/10.4012/dmj.2019-288
doi: 10.4012/dmj.2019-288
Miletić I, Devcić N, Anić I, Borcić J, Karlović Z, Osmak M (2005) The cytotoxicity of RoekoSeal and AH plus compared during different setting periods. J Endod 31(4):307–309. https://doi.org/10.1097/01.don.0000140570.95688.ee
doi: 10.1097/01.don.0000140570.95688.ee
pubmed: 15793391
Silva EJ, Accorsi-Mendonça T, Almeida JF, Ferraz CC, Gomes BP, Zaia AA (2012) Evaluation of cytotoxicity and up-regulation of gelatinases in human fibroblast cells by four root canal sealers. Int Endod J 45(1):49–56. https://doi.org/10.1111/j.1365-2591.2011.01946.x
doi: 10.1111/j.1365-2591.2011.01946.x
pubmed: 21910744
Jung S, Sielker S, Hanisch MR, Libricht V, Schäfer E, Dammaschke T (2018) Cytotoxic effects of four different root canal sealers on human osteoblasts. PLoS ONE 26(3):e0194467. https://doi.org/10.1371/journal.pone.0194467
doi: 10.1371/journal.pone.0194467
Souza LC, Neves GST, Kirkpatrick T, Letra A, Silva R (2023) Physicochemical and Biological properties of AH Plus Bioceramic. J Endod 49(1):69–76. https://doi.org/10.1016/j.joen.2022.10.009
doi: 10.1016/j.joen.2022.10.009
pubmed: 36279961
Gaudin A, Tolar M, Peters OA (2020) Cytokine Production and Cytotoxicity of Calcium Silicate-based Sealers in 2- and 3-dimensional Cell Culture Models. J Endod 46(6):818–826. https://doi.org/10.1016/j.joen.2020.03.011 . Epub
da Silva EJNL, Zaia AA, Peters OA (2017) Cytocompatibility of calcium silicate-based sealers in a three-dimensional cell culture model. Clin Oral Investig 21(5):1531–1536. https://doi.org/10.1007/s00784-016-1918-9
doi: 10.1007/s00784-016-1918-9
pubmed: 27460565
Scelza MZ, Linhares AB, da Silva LE, Granjeiro JM, Alves GG (2012) A multiparametric assay to compare the cytotoxicity of endodontic sealers with primary human osteoblasts. Int Endod J 45(1):12–18. https://doi.org/10.1111/j.1365-2591.2011.01941.x
doi: 10.1111/j.1365-2591.2011.01941.x
pubmed: 21902702
Restle L, Costa-Silva D, Lourenço ES, Bachinski RF, Batista AC, Linhares ABR, Alves GG (2015) A 3D osteoblast in vitro model for the evaluation of biomedical materials. Adv Mater Sci Eng. https://doi.org/10.1155/2015/268930
doi: 10.1155/2015/268930
Brochado ACB, Silva DC, Silva JCd, Lowenstein A, Gameiro VS, Mavropoulos E, Mourão CF, Alves GG (2023) Characterization and applicability of a bone spheroid model for the evaluation of cytocompatibility of bone substitutes. Appl Sci 13(3):1602. https://doi.org/10.3390/app13031602
doi: 10.3390/app13031602
Maia-Pinto MOC, Brochado ACB, Teixeira BN, Sartoretto SC, Uzeda MJ, Alves ATNN, Alves GG, Calasans-Maia MD, Thiré RMSM (2020) Biomimetic mineralization on 3D printed PLA scaffolds: on the response of human primary osteoblasts spheroids and in vivo implantation. Polym (Basel) 13(1):74. https://doi.org/10.3390/polym13010074
doi: 10.3390/polym13010074
ISO International Organization for Standardization (2007) ISO 10993-12:2007 Biological evaluation of Medical Devices—Part 12: Sample Preparation and Reference materials. International Organization for Standardization, Geneva
Aminoshariae A, Kulild JC (2020) The impact of sealer extrusion on endodontic outcome: a systematic review with meta-analysis. Aust Endod J 46(1):123–129. https://doi.org/10.1111/aej.12370
doi: 10.1111/aej.12370
pubmed: 31449355
Teixeira ABV, Moreira NCS, Takahashi CS, Schiavon MA, Alves OL, Reis AC (2021) Cytotoxic and genotoxic effects in human gingival fibroblast and ions release of endodontic sealers incorporated with nanostructured silver vanadate. J Biomed Mater Res B Appl Biomater 109(9):1380–1388. https://doi.org/10.1002/jbm.b.34798
doi: 10.1002/jbm.b.34798
pubmed: 33470054
Shokrzadeh M, Motafeghi FS, Lotfizadeh A, Ghorbani M, Haddadi Kohsar A (2023) Cytotoxicity and mineralization activity of Calcium Silicate-based Root Canal Sealers compared to Conventional Resin-based Sealer in Human Gingival Fibroblast cells. Int J Dent. https://doi.org/10.1155/2023/4376579 . 31;2023:4376579
doi: 10.1155/2023/4376579
pubmed: 37293576
pmcid: 10247329
Brackett MG, Lewis JB, Messer RL, Lei L, Lockwood PE, Wataha JC (2011) Dysregulation of monocytic cytokine secretion by endodontic sealers. J Biomed Mater Res B Appl Biomater 97(1):49–57. https://doi.org/10.1002/jbm.b.31785
doi: 10.1002/jbm.b.31785
pubmed: 21290574
Silva EJ, Hecksher F, Vieira VT, Vivan RR, Duarte MA, Brasil SC, Antunes HS (2020) Cytotoxicity, antibacterial and physicochemical properties of a new epoxy resin-based endodontic sealer containing calcium hydroxide. J Clin Exp Dent 1;12(6):e533-e539. https://doi.org/10.4317/jced.56534
ISO International Organization for Standardization (2018) ISO 10993-12:2018 Biological evaluation of Medical Devices—Part 12: Sample Preparation and Reference materials. International Organization for Standardization, Geneva
Vouzara T, Dimosiari G, Koulaouzidou EA, Economides N (2018) Cytotoxicity of a New Calcium Silicate Endodontic Sealer. J Endod 44(5):849–852. https://doi.org/10.1016/j.joen.2018.01.015
doi: 10.1016/j.joen.2018.01.015
pubmed: 29550005
Yang X, Zheng T, Yang N, Yin Z, Wang W, Bai Y (2022) A review of the research methods and progress of biocompatibility evaluation of root canal sealers. Aust Endod J 49 Suppl 1508–514. https://doi.org/10.1111/aej.12725
Jung S, Libricht V, Sielker S, Hanisch MR, Schäfer E, Dammaschke T (2019) Evaluation of the biocompatibility of root canal sealers on human periodontal ligament cells ex vivo. Odontology 107(1):54–63. https://doi.org/10.1007/s10266-018-0380-3
doi: 10.1007/s10266-018-0380-3
pubmed: 30039235
Lee BN, Hong JU, Kim SM, Jang JH, Chang HS, Hwang YC, Hwang IN, Oh WM (2019) Anti-inflammatory and Osteogenic Effects of Calcium Silicate-based Root Canal Sealers. J Endod 45(1):73–78. https://doi.org/10.1016/j.joen.2018.09.006
doi: 10.1016/j.joen.2018.09.006
pubmed: 30558800
Zhu Q, Haglund R, Safavi KE, Spangberg LS (2000) Adhesion of human osteoblasts on root-end filling materials. J Endod 26(7):404–406. https://doi.org/10.1097/00004770-200007000-00006
doi: 10.1097/00004770-200007000-00006
pubmed: 11199765
Pautke C, Schieker M, Tischer T, Kolk A, Neth P, Mutschler W, Milz S (2004) Characterization of osteosarcoma cell lines MG-63, Saos-2 and U-2 OS in comparison to human osteoblasts. Anticancer Res 24(6):3743–3748
pubmed: 15736406
Czekanska EM, Stoddart MJ, Ralphs JR, Richards RG, Hayes JS (2014) A phenotypic comparison of osteoblast cell lines versus human primary osteoblasts for biomaterials testing. J Biomed Mater Res A 102(8):2636–2643. https://doi.org/10.1002/jbm.a.34937
doi: 10.1002/jbm.a.34937
pubmed: 23983015
de Lima Barbosa R, Rodrigues Santiago Rocha N, Stellet Lourenço E, de Souza Lima VH, Mavropoulos E, Mello-Machado RC, Spiegel C, Mourão CF, Alves GG (2024) The Association of Nanostructured Carbonated Hydroxyapatite with denatured albumin and platelet-rich fibrin: impacts on growth factors release and osteoblast behavior. J Funct Biomater 5(1):18. https://doi.org/10.3390/jfb15010018
doi: 10.3390/jfb15010018
Staehlke S, Rebl H, Finke B, Mueller P, Gruening M, Nebe JB (2018) Enhanced calcium ion mobilization in osteoblasts on amino group containing plasma polymer nanolayer. Cell Biosci 21:8:22. https://doi.org/10.1186/s13578-018-0220-8
doi: 10.1186/s13578-018-0220-8
Chalak M, Hesaraki M, Mirbahari SN, Yeganeh M, Abdi S, Rajabi S, Hemmatzadeh F (2024) Cell immortality: in Vitro Effective techniques to achieve and investigate its Applications and challenges. Life (Basel) 21(143):417. https://doi.org/10.3390/life14030417
doi: 10.3390/life14030417
da Silva EJNL, de Carvalho NK, Zaia AA (2016) Cytotoxicity profile of epoxy resin sealer provided by a new experimental 3D cell culture model. ENDO 10(1)
Wataha JC, Nakajima H, Hanks CT, Okabe T (1994) Correlation of cytotoxicity with element release from mercury- and gallium-based dental alloys in vitro. Dent Mater 10(5):298–303. https://doi.org/10.1016/0109-5641(94)90037-x
doi: 10.1016/0109-5641(94)90037-x
pubmed: 7498590
Silva EJ, Carvalho NK, Ronconi CT, De-Deus G, Zuolo ML, Zaia AA (2016) Cytotoxicity Profile of Endodontic Sealers provided by 3D cell culture experimental model. Braz Dent J 27(6):652–656. https://doi.org/10.1590/0103-6440201600792
doi: 10.1590/0103-6440201600792
pubmed: 27982174
Brochado ACB, de Souza VH, Correa J, Dos Anjos SA, de Almeida Barros Mourão CF, Cardarelli A, Montemezzi P, Gameiro VS, Pereira MR, Mavropoulos E, Alves GG (2021) Osteosphere Model to Evaluate Cell-Surface Interactions of Implantable Biomaterials. Materials (Basel) 7;14(19):5858. https://doi.org/10.3390/ma14195858
Piai GG, Duarte MAH, Nascimento ALD, Rosa RAD, Só MVR, Vivan RR (2018) Penetrability of a new endodontic sealer: a confocal laser scanning microscopy evaluation. Microsc Res Tech 81(11):1246–1249. https://doi.org/10.1002/jemt.23129
doi: 10.1002/jemt.23129
pubmed: 30295382
Cintra LTA, Benetti F, de Azevedo Queiroz ÍO, Ferreira LL, Massunari L, Bueno CRE, de Oliveira SHP, Gomes-Filho JE (2017) Evaluation of the cytotoxicity and biocompatibility of New Resin Epoxy-based Endodontic Sealer Containing Calcium Hydroxide. J Endod 43(12):2088–2092. https://doi.org/10.1016/j.joen.2017.07.016
doi: 10.1016/j.joen.2017.07.016
pubmed: 29032822
Seron MA, Nunes GP, Ferrisse TM, Strazzi-Sahyon HB, Victorino FR, Dos Santos PH, Gomes-Filho JE, Cintra LTA, Sivieri-Araujo G (2023) Postoperative pain after root canal filling with bioceramic sealers: a systematic review and meta-analysis of randomized clinical trials. Odontology 111(4):793–812. https://doi.org/10.1007/s10266-023-00830-z
doi: 10.1007/s10266-023-00830-z
pubmed: 37378833
Mendes AT, Weissheimer T, Tietz L, Só BB, Só GB, da Rosa RA, Só MVR (2023) Postoperative pain incidence and intensity following root canal obturation with bioceramic and other sealers: a systematic review with meta-analysis. J Den Res Dentistry 11(2):32–51
doi: 10.59306/jrd.v11e2202332-51
Radwanski M, Rozpedek-Kaminska W, Galita G, Siwecka N, Sokolowski J, Majsterek I, Özcan M, Lukomska-Szymanska M (2024) Cytotoxicity and genotoxicity of bioceramic root canal sealers compared to conventional resin-based sealer. Sci Rep 19(1):4124. https://doi.org/10.1038/s41598-024-54726-1
doi: 10.1038/s41598-024-54726-1
Kwak SW, Koo J, Song M, Jang IH, Gambarini G, Kim HC (2023) Physicochemical Properties and Biocompatibility of various Bioceramic Root Canal Sealers: in Vitro Study. J Endod 49(7):871–879. https://doi.org/10.1016/j.joen.2023.05.013
doi: 10.1016/j.joen.2023.05.013
pubmed: 37225037
Khalil I, Naaman A, Camilleri J (2016) Properties of Tricalcium Silicate Sealers. J Endod. 2016;42(10):1529-35. https://doi.org/10.1016/j.joen.2016.06.002
Martorano AS, Messias NS, Bighetti-Trevisan RL, de Oliveira PT, de Castro Raucci LMS, Raucci Neto W (2022) In vitro inflammatory modulation of bioceramic endodontic sealer in macrophages stimulated by bacterial lipopolysaccharide. Int Endod J 56(2):213–226. https://doi.org/10.1111/iej.13858
doi: 10.1111/iej.13858
pubmed: 36314853
Guo J, Peters OA (2023) Hosseinpour S. Immunomodulatory Effects of Endodontic Sealers: A Systematic Review. Dent J (Basel). 2023;11(2):54. https://doi.org/10.3390/dj11020054
Tyler LW, Matossian K, Todd R, Gallagher GT, White RR, Wong DT (1999) Eosinophil-derived transforming growth factors (TGF-alpha and TGF-beta 1) in human periradicular lesions. J Endod 25(9):619–624. https://doi.org/10.1016/S0099-2399(99)80322-7
doi: 10.1016/S0099-2399(99)80322-7
pubmed: 10687542
Andreev D, Kachler K, Liu M, Chen Z, Krishnacoumar B, Ringer M, Frey S, Krönke G, Voehringer D, Schett G, Bozec A (2024) Eosinophils preserve bone homeostasis by inhibiting excessive osteoclast formation and activity via eosinophil peroxidase. Nat Commun 15(1):1067. https://doi.org/10.1038/s41467-024-45261-8
doi: 10.1038/s41467-024-45261-8
pubmed: 38316791
pmcid: 10844633
Acheampong I, Minadzi D, Adankwah E, Aniagyei W, Vivekanandan MM, Yeboah A, Arthur JF, Lamptey M, Abass MK, Kumbel F, Osei-Yeboah F, Gawusu A, Laing EF, Batsa Debrah L, Owusu DO, Debrah A, Mayatepek E, Seyfarth J, Phillips RO, Jacobsen M (2023) Diminished Interleukin-7 receptor expression on T-cell subsets in tuberculosis patients. Hum Immunol 84(10):543–550. https://doi.org/10.1016/j.humimm.2023.08.141
doi: 10.1016/j.humimm.2023.08.141
pubmed: 37580215
Kwak HB, Ha H, Kim HN, Lee JH, Kim HS, Lee S, Kim HM, Kim JY, Kim HH, Song YW, Lee ZH (2008) Reciprocal cross-talk between RANKL and interferon-gamma-inducible protein 10 is responsible for bone-erosive experimental arthritis. Arthritis Rheum 58(5):1332–1342. https://doi.org/10.1002/art.23372
doi: 10.1002/art.23372
pubmed: 18438854
Hadziabdic N, Kurtovic-Kozaric A, Frkatovic A, Smajlovic S, Letra A (2019) Quantitative analysis of CCL5 and ep300 in Periapical Inflammatory lesions. Acta Med Acad 48(2):129–139. https://doi.org/10.5644/ama2006-124.251
doi: 10.5644/ama2006-124.251
pubmed: 31718213
Silva TA, Garlet GP, Lara VS, Martins W Jr, Silva JS, Cunha FQ (2005) Differential expression of chemokines and chemokine receptors in inflammatory periapical diseases. Oral Microbiol Immunol 20(5):310–316. https://doi.org/10.1111/j.1399-302X.2005.00232.x
doi: 10.1111/j.1399-302X.2005.00232.x
pubmed: 16101967
Gomes-Filho JE, Watanabe S, Gomes AC, Faria MD, Lodi CS, Penha Oliveira SH (2009) Evaluation of the effects of endodontic materials on fibroblast viability and cytokine production. J Endod 35(11):1577–1579. https://doi.org/10.1016/j.joen.2009.07.022
doi: 10.1016/j.joen.2009.07.022
pubmed: 19840651
Sanz JL, Guerrero-Gironés J, Pecci-Lloret MP, Pecci-Lloret MR, Melo M (2021) Biological interactions between calcium silicate-based endodontic biomaterials and periodontal ligament stem cells: a systematic review of in vitro studies. Int Endod J 54(11):2025–2043. https://doi.org/10.1111/iej.13600
doi: 10.1111/iej.13600
pubmed: 34338339
Hosseinpour S, Gaudin A, Peters OA (2022) A critical analysis of research methods and experimental models to study biocompatibility of endodontic materials. Int Endod J 55 Suppl 2(Suppl 2):346–369. https://doi.org/10.1111/iej.13701
doi: 10.1111/iej.13701