Anti-metastasis activity of 5,4'-dihydroxy 6,8-dimethoxy 7-O-rhamnosyl flavone from Indigofera aspalathoides Vahl on breast cancer cells.
Humans
Breast Neoplasms
/ drug therapy
Epithelial-Mesenchymal Transition
/ drug effects
Female
Cell Line, Tumor
Flavones
/ pharmacology
Indigofera
/ chemistry
Cell Movement
/ drug effects
Signal Transduction
/ drug effects
Neoplasm Metastasis
Proto-Oncogene Proteins c-akt
/ metabolism
NF-kappa B
/ metabolism
Plant Extracts
/ pharmacology
Indigofera aspalathoides
5,4’-Dihydroxy-6,8-dimethoxy-7-O-rhamnosyl
Breast cancer
PI3K/AKT
Wnt/β-catenin
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
29 May 2024
29 May 2024
Historique:
received:
10
12
2023
accepted:
24
05
2024
medline:
30
5
2024
pubmed:
30
5
2024
entrez:
29
5
2024
Statut:
epublish
Résumé
Breast cancer presents a significant challenge due to its high rates of illness and mortality, necessitating more effective treatment approaches. While traditional treatments offer some benefits, they often lack precision in targeting cancer cells and can inadvertently harm healthy tissues. This study aims to investigate the cytotoxic effects and molecular mechanism of 5,4'-dihydroxy-6,8-dimethoxy-7-O-rhamnosyl flavone (DDR), extracted from Indigofera aspalathoides Vahl, on breast cancer cells (MDA-MB-231). Through various in vitro assays including wound healing, invasion, Western blotting, and immunofluorescence, the impact of DDR on epithelial-mesenchymal transition (EMT) and metastasis was evaluated. Treatment of MDA-MB-231 cells with different DDR concentrations (0-10 µg/mL) resulted in a significant decrease in invasion and migration, accompanied by the downregulation of metastasis-related proteins including VEGF, uPAR, uPA, and MMP-9. DDR treatment also hindered EMT by upregulating E-cadherin and downregulating N-cadherin, Slug, Twist, and Vimentin. Additionally, inhibition of the PI3K/AKT signaling pathway and downregulation of the NF-кB pathway were observed. These findings highlight the potential of DDR as a valuable source of natural compounds with promising anticancer properties, offering opportunities for the development of novel cancer therapies.
Identifiants
pubmed: 38811791
doi: 10.1038/s41598-024-63136-2
pii: 10.1038/s41598-024-63136-2
doi:
Substances chimiques
Flavones
0
Proto-Oncogene Proteins c-akt
EC 2.7.11.1
NF-kappa B
0
Plant Extracts
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
12349Subventions
Organisme : Kuwait University-Research Sector
ID : [MN01/20]
Organisme : Kuwait University-Research Sector
ID : [MN01/20]
Informations de copyright
© 2024. The Author(s).
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