Humans Class I Phosphatidylinositol 3-Kinases / genetics Saliva Mouth Neoplasms / genetics Mutation Biomarkers, Tumor / genetics Female Male Middle Aged Aged Carcinoma, Squamous Cell / genetics DNA Mutational Analysis / methods Aged, 80 and over Adult
OSCC Oral cancer PIK3CA ctDNA hotspot mutations liquid biopsies mass spectrometry saliva

Journal

Anticancer research
ISSN: 1791-7530
Titre abrégé: Anticancer Res
Pays: Greece
ID NLM: 8102988

Informations de publication

Date de publication:
Jun 2024
Historique:
received: 27 03 2024
revised: 19 04 2024
accepted: 22 04 2024
medline: 1 6 2024
pubmed: 1 6 2024
entrez: 31 5 2024
Statut: ppublish

Résumé

This study aimed at the analogous detection of PIK3CA mutations, common in oral squamous cell carcinoma (OSCC), in matched tumor and saliva samples. Tissue and saliva samples were obtained from 29 patients diagnosed with primary OSCC. Saliva samples were obtained preoperatively; tissue specimens were acquired during tumor resection. Tumor DNA was extracted from both tissue and saliva samples. All samples were controlled for DNA quantity and quality and genetic matching of sample pairs was confirmed using the iPlex Pro Exome QC Panel. Variant detection was performed using the MassARRAY Overall, a PIK3CA variant was found in seven of the 29 tumor tissue samples (24%) by ClearSEEK™; UltraSEEK

Sections du résumé

BACKGROUND/AIM OBJECTIVE
This study aimed at the analogous detection of PIK3CA mutations, common in oral squamous cell carcinoma (OSCC), in matched tumor and saliva samples.
PATIENTS AND METHODS METHODS
Tissue and saliva samples were obtained from 29 patients diagnosed with primary OSCC. Saliva samples were obtained preoperatively; tissue specimens were acquired during tumor resection. Tumor DNA was extracted from both tissue and saliva samples. All samples were controlled for DNA quantity and quality and genetic matching of sample pairs was confirmed using the iPlex Pro Exome QC Panel. Variant detection was performed using the MassARRAY
RESULTS RESULTS
Overall, a PIK3CA variant was found in seven of the 29 tumor tissue samples (24%) by ClearSEEK™; UltraSEEK

Identifiants

DOI: 10.21873/anticanres.17044 PMID: 38821622
pubmed: 38821622
pii: 44/6/2369
doi: 10.21873/anticanres.17044
doi:

Substances chimiques

PIK3CA protein, human EC 2.7.1.137
Class I Phosphatidylinositol 3-Kinases EC 2.7.1.137
Biomarkers, Tumor 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

2369-2376

Informations de copyright

Copyright © 2024 International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.

Auteurs

Konstantin Krüger (K)

Department of Oral and Maxillofacial Surgery, University Medical Center Hamburg-Eppendorf, Hamburg, Germany; konkruger@gmail.com.

Audrey Grust (A)

Department of Oral and Maxillofacial Surgery, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
Department of Oral and Maxillofacial Surgery, Division of Regenerative Orofacial Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.

David Muallah (D)

Department of Oral and Maxillofacial Surgery, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
Department of Oral and Maxillofacial Surgery, Division of Regenerative Orofacial Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.

Adrian Patenge (A)

Department of Oral and Maxillofacial Surgery, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.

Harriet Wikman (H)

Institute of Tumour Biology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.

Maria Medina Mesa (MM)

Agena Bioscience GmbH, Hamburg, Germany.

Eike Sören Knust (ES)

Agena Bioscience GmbH, Hamburg, Germany.

Alexander Sartori (A)

Agena Bioscience GmbH, Hamburg, Germany.

Ralf Smeets (R)

Department of Oral and Maxillofacial Surgery, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
Department of Oral and Maxillofacial Surgery, Division of Regenerative Orofacial Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.

Lan Kluwe (L)

Department of Oral and Maxillofacial Surgery, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
Laboratory for Tumour Genetics and Regenerative Medicine, Department of Neurology, University Hospital Hamburg-Eppendorf, Hamburg, Germany.

Reinhard E Friedrich (RE)

Department of Oral and Maxillofacial Surgery, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.

Simon Burg (S)

Department of Oral and Maxillofacial Surgery, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains ›
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines et peptides de signalisation intracellulaire › Phosphatidylinositol 3-kinases › Phosphatidylinositol-4-phosphate 3-kinase › Phosphatidylinositol 3-kinases de classe I ›
questionsmedicales.fr › Liquides et sécrétions biologiques › Sécrétions corporelles › Salive ›
questionsmedicales.fr › Maladies du système stomatognathique › Maladies de la bouche › Tumeurs de la bouche ›
questionsmedicales.fr › Phénomènes génétiques › Variation génétique › Mutation ›
questionsmedicales.fr › Facteurs biologiques › Marqueurs biologiques › Marqueurs biologiques tumoraux ›
questionsmedicales.fr › Personnes › Tranches d'âge › Adulte › Adulte d'âge moyen ›
questionsmedicales.fr › Personnes › Tranches d'âge › Adulte › Sujet âgé ›
questionsmedicales.fr › Tumeurs › Tumeurs par type histologique › Tumeurs épithéliales épidermoïdes et glandulaires › Tumeurs épidermoïdes › Carcinome épidermoïde ›
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Analyse de séquence › Analyse de séquence d'ADN › Analyse de mutations d'ADN ›
questionsmedicales.fr › Personnes › Tranches d'âge › Adulte › Sujet âgé › Sujet âgé de 80 ans ou plus ›
questionsmedicales.fr › Personnes › Tranches d'âge › Adulte ›