The adenosine A2A receptor in human sperm: its role in sperm motility and association with
Humans
Male
Sperm Motility
/ drug effects
Receptor, Adenosine A2A
/ metabolism
Spermatozoa
/ metabolism
Fertilization in Vitro
/ methods
Adult
Asthenozoospermia
/ metabolism
Female
Pyrazoles
/ pharmacology
Adenosine A2 Receptor Agonists
/ pharmacology
Adenosine A2 Receptor Antagonists
/ pharmacology
Pyrimidines
/ pharmacology
Triazoles
/ pharmacology
CatSper
adenosine A2A receptor
in vitro fertilization
intracellular calcium concentration
sperm motility
Journal
Frontiers in endocrinology
ISSN: 1664-2392
Titre abrégé: Front Endocrinol (Lausanne)
Pays: Switzerland
ID NLM: 101555782
Informations de publication
Date de publication:
2024
2024
Historique:
received:
01
04
2024
accepted:
13
05
2024
medline:
14
6
2024
pubmed:
14
6
2024
entrez:
14
6
2024
Statut:
epublish
Résumé
The involvement of ATP and cAMP in sperm function has been extensively documented, but the understanding of the role of adenosine and adenosine receptors remains incomplete. This study aimed to examine the presence of adenosine A2A receptor (A2AR) and study the functional role of A2AR in human sperm. The presence and localization of A2AR in human sperm were examined by western blotting and immunofluorescence assays. The functional role of A2AR in sperm was assessed by incubating human sperm with an A2AR agonist (regadenoson) and an A2AR antagonist (SCH58261). The sperm level of A2AR was examined by western blotting in normozoospermic and asthenozoospermic men to evaluate the association of A2AR with sperm motility and A2AR with a molecular weight of 43 kDa was detected in the tail of human sperm. SCH58261 decreased the motility, penetration ability, intracellular Ca These results indicate that A2AR is important for human sperm motility and is associated with IVF outcome.
Sections du résumé
Background
UNASSIGNED
The involvement of ATP and cAMP in sperm function has been extensively documented, but the understanding of the role of adenosine and adenosine receptors remains incomplete. This study aimed to examine the presence of adenosine A2A receptor (A2AR) and study the functional role of A2AR in human sperm.
Methods
UNASSIGNED
The presence and localization of A2AR in human sperm were examined by western blotting and immunofluorescence assays. The functional role of A2AR in sperm was assessed by incubating human sperm with an A2AR agonist (regadenoson) and an A2AR antagonist (SCH58261). The sperm level of A2AR was examined by western blotting in normozoospermic and asthenozoospermic men to evaluate the association of A2AR with sperm motility and
Results
UNASSIGNED
A2AR with a molecular weight of 43 kDa was detected in the tail of human sperm. SCH58261 decreased the motility, penetration ability, intracellular Ca
Conclusions
UNASSIGNED
These results indicate that A2AR is important for human sperm motility and is associated with IVF outcome.
Identifiants
pubmed: 38872963
doi: 10.3389/fendo.2024.1410370
pmc: PMC11169588
doi:
Substances chimiques
Receptor, Adenosine A2A
0
Pyrazoles
0
5-amino-7-(2-phenylethyl)-2-(2-furyl)pyrazolo(4,3-e)-1,2,4-triazolo(1,5-c)pyrimidine
0
Adenosine A2 Receptor Agonists
0
Adenosine A2 Receptor Antagonists
0
Pyrimidines
0
Triazoles
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1410370Informations de copyright
Copyright © 2024 Chen, Xing, Xu, Chen, Xia, Huang, Huang, Hong, Luo, Wang and Wu.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.