3D bioprinted mesenchymal stem cell laden scaffold enhances subcutaneous vascularization for delivery of cell therapy.
Mesenchymal Stem Cells
/ cytology
Animals
Tissue Scaffolds
/ chemistry
Alginates
/ chemistry
Neovascularization, Physiologic
Printing, Three-Dimensional
Bioprinting
Rats
Gelatin
/ chemistry
Mesenchymal Stem Cell Transplantation
Cell- and Tissue-Based Therapy
Subcutaneous Tissue
Rats, Sprague-Dawley
Hydrogels
/ chemistry
3D bioprinting
Cell therapy
Mesenchymal stem cells
Regenerative medicine
Tissue engineering
Vascularized scaffold
Journal
Biomedical microdevices
ISSN: 1572-8781
Titre abrégé: Biomed Microdevices
Pays: United States
ID NLM: 100887374
Informations de publication
Date de publication:
18 Jun 2024
18 Jun 2024
Historique:
accepted:
10
06
2024
medline:
18
6
2024
pubmed:
18
6
2024
entrez:
18
6
2024
Statut:
epublish
Résumé
Subcutaneous delivery of cell therapy is an appealing minimally-invasive strategy for the treatment of various diseases. However, the subdermal site is poorly vascularized making it inadequate for supporting engraftment, viability, and function of exogenous cells. In this study, we developed a 3D bioprinted scaffold composed of alginate/gelatin (Alg/Gel) embedded with mesenchymal stem cells (MSCs) to enhance vascularization and tissue ingrowth in a subcutaneous microenvironment. We identified bio-ink crosslinking conditions that optimally recapitulated the mechanical properties of subcutaneous tissue. We achieved controlled degradation of the Alg/Gel scaffold synchronous with host tissue ingrowth and remodeling. Further, in a rat model, the Alg/Gel scaffold was superior to MSC-embedded Pluronic hydrogel in supporting tissue development and vascularization of a subcutaneous site. While the scaffold alone promoted vascular tissue formation, the inclusion of MSCs in the bio-ink further enhanced angiogenesis. Our findings highlight the use of simple cell-laden degradable bioprinted structures to generate a supportive microenvironment for cell delivery.
Identifiants
pubmed: 38888669
doi: 10.1007/s10544-024-00713-2
pii: 10.1007/s10544-024-00713-2
doi:
Substances chimiques
Alginates
0
Gelatin
9000-70-8
Hydrogels
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
29Subventions
Organisme : NIDDK NIH HHS
ID : R01DK132104
Pays : United States
Organisme : Juvenile Diabetes Research Foundation United States of America
ID : 2-SRA-2022-1224-S-B
Informations de copyright
© 2024. The Author(s).
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