A single-cell atlas of pig gastrulation as a resource for comparative embryology.
Animals
Gastrulation
Endoderm
/ cytology
Swine
Single-Cell Analysis
Gene Expression Regulation, Developmental
Mice
Embryo, Mammalian
/ cytology
Cell Differentiation
Mesoderm
/ cytology
Transcriptome
Hepatocyte Nuclear Factor 3-beta
/ metabolism
Cell Lineage
T-Box Domain Proteins
/ metabolism
Epithelial-Mesenchymal Transition
/ genetics
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
18 Jun 2024
18 Jun 2024
Historique:
received:
21
08
2023
accepted:
04
06
2024
medline:
19
6
2024
pubmed:
19
6
2024
entrez:
18
6
2024
Statut:
epublish
Résumé
Cell-fate decisions during mammalian gastrulation are poorly understood outside of rodent embryos. The embryonic disc of pig embryos mirrors humans, making them a useful proxy for studying gastrulation. Here we present a single-cell transcriptomic atlas of pig gastrulation, revealing cell-fate emergence dynamics, as well as conserved and divergent gene programs governing early porcine, primate, and murine development. We highlight heterochronicity in extraembryonic cell-types, despite the broad conservation of cell-type-specific transcriptional programs. We apply these findings in combination with functional investigations, to outline conserved spatial, molecular, and temporal events during definitive endoderm specification. We find early FOXA2 + /TBXT- embryonic disc cells directly form definitive endoderm, contrasting later-emerging FOXA2/TBXT+ node/notochord progenitors. Unlike mesoderm, none of these progenitors undergo epithelial-to-mesenchymal transition. Endoderm/Node fate hinges on balanced WNT and hypoblast-derived NODAL, which is extinguished upon endodermal differentiation. These findings emphasise the interplay between temporal and topological signalling in fate determination during gastrulation.
Identifiants
pubmed: 38890321
doi: 10.1038/s41467-024-49407-6
pii: 10.1038/s41467-024-49407-6
doi:
Substances chimiques
Hepatocyte Nuclear Factor 3-beta
135845-92-0
T-Box Domain Proteins
0
Types de publication
Journal Article
Comparative Study
Langues
eng
Sous-ensembles de citation
IM
Pagination
5210Subventions
Organisme : RCUK | Biotechnology and Biological Sciences Research Council (BBSRC)
ID : BB/S000178/1
Organisme : RCUK | Biotechnology and Biological Sciences Research Council (BBSRC)
ID : BB/T013575/1
Informations de copyright
© 2024. Crown.
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