Interplay of two small RNAs fine-tunes hierarchical flagella gene expression in Campylobacter jejuni.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
19 Jun 2024
19 Jun 2024
Historique:
received:
06
07
2023
accepted:
13
05
2024
medline:
20
6
2024
pubmed:
20
6
2024
entrez:
19
6
2024
Statut:
epublish
Résumé
Like for many bacteria, flagella are crucial for Campylobacter jejuni motility and virulence. Biogenesis of the flagellar machinery requires hierarchical transcription of early, middle (RpoN-dependent), and late (FliA-dependent) genes. However, little is known about post-transcriptional regulation of flagellar biogenesis by small RNAs (sRNAs). Here, we characterized two sRNAs with opposing effects on C. jejuni filament assembly and motility. We demonstrate that CJnc230 sRNA (FlmE), encoded downstream of the flagellar hook protein, is processed from the RpoN-dependent flgE mRNA by RNase III, RNase Y, and PNPase. We identify mRNAs encoding a flagella-interaction regulator and the anti-sigma factor FlgM as direct targets of CJnc230 repression. CJnc230 overexpression upregulates late genes, including the flagellin flaA, culminating in longer flagella and increased motility. In contrast, overexpression of the FliA-dependent sRNA CJnc170 (FlmR) reduces flagellar length and motility. Overall, our study demonstrates how the interplay of two sRNAs post-transcriptionally fine-tunes flagellar biogenesis through balancing of the hierarchically-expressed components.
Identifiants
pubmed: 38897989
doi: 10.1038/s41467-024-48986-8
pii: 10.1038/s41467-024-48986-8
doi:
Substances chimiques
Bacterial Proteins
0
RNA, Bacterial
0
RNA, Small Untranslated
0
Flagellin
12777-81-0
RNA, Messenger
0
FlgM protein, Bacteria
142462-45-1
Ribonuclease III
EC 3.1.26.3
flaA protein, bacteria
133606-66-3
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
5240Subventions
Organisme : Deutsche Forschungsgemeinschaft (German Research Foundation)
ID : GRK2157
Informations de copyright
© 2024. The Author(s).
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