Development of a biotechnology process for the production of a novel hyperglycosylated long-acting recombinant bovine follicle-stimulating hormone.
O‐glycosylation
bioprocess
glycoengineering
glycoprotein hormone
pharmacokinetics
Journal
Biotechnology journal
ISSN: 1860-7314
Titre abrégé: Biotechnol J
Pays: Germany
ID NLM: 101265833
Informations de publication
Date de publication:
Jun 2024
Jun 2024
Historique:
revised:
17
05
2024
received:
17
04
2024
accepted:
28
05
2024
medline:
20
6
2024
pubmed:
20
6
2024
entrez:
20
6
2024
Statut:
ppublish
Résumé
Follicle-stimulating hormone (FSH) is an important protein used for bovine ovarian hyperstimulation in multiple ovulation and embryo transfer technology (MOET). Several attempts to produce bovine FSH (bFSH) in recombinant systems have been reported, nonetheless, up to date, the most commonly used products are partially purified preparations derived from porcine or ovine (pFSH or oFSH) pituitaries. Here we describe the development of a biotechnology process to produce a novel, hyperglycosylated, long-acting recombinant bFSH (LA-rbFSH) by fusing copies of a highly O-glycosylated peptide. LA-rbFSH and a nonmodified version (rbFSH) were produced in suspension CHO cell cultures and purified by IMAC with high purity levels (>99%). LA-rbFSH presented a higher glycosylation degree and sialic acid content than rbFSH. It also demonstrated a notable improvement in pharmacokinetic properties after administration to rats, including a higher concentration in plasma and a significant (seven-fold) reduction in apparent clearance (CL
Identifiants
pubmed: 38900054
doi: 10.1002/biot.202400260
doi:
Substances chimiques
Follicle Stimulating Hormone
9002-68-0
Recombinant Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2400260Subventions
Organisme : Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)
Organisme : Ministerio de Desarrollo Productivo
Organisme : Universidad Nacional del Litoral (UNL)
Informations de copyright
© 2024 Wiley‐VCH GmbH.
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