The cytoplasmic phosphate level has a central regulatory role in the phosphate starvation response of Caulobacter crescentus.
Journal
Communications biology
ISSN: 2399-3642
Titre abrégé: Commun Biol
Pays: England
ID NLM: 101719179
Informations de publication
Date de publication:
26 Jun 2024
26 Jun 2024
Historique:
received:
07
10
2023
accepted:
19
06
2024
medline:
27
6
2024
pubmed:
27
6
2024
entrez:
26
6
2024
Statut:
epublish
Résumé
In bacteria, the availability of environmental inorganic phosphate is typically sensed by the conserved PhoR-PhoB two-component signal transduction pathway, which uses the flux through the PstSCAB phosphate transporter as a readout of the extracellular phosphate level to control phosphate-responsive genes. While the sensing of environmental phosphate is well-investigated, the regulatory effects of cytoplasmic phosphate are unclear. Here, we disentangle the physiological and transcriptional responses of Caulobacter crescentus to changes in the environmental and cytoplasmic phosphate levels by uncoupling phosphate uptake from the activity of the PstSCAB system, using an additional, heterologously produced phosphate transporter. This approach reveals a two-pronged response of C. crescentus to phosphate limitation, in which PhoR-PhoB signaling mostly facilitates the utilization of alternative phosphate sources, whereas the cytoplasmic phosphate level controls the morphological and physiological adaptation of cells to growth under global phosphate limitation. These findings open the door to a comprehensive understanding of phosphate signaling in bacteria.
Identifiants
pubmed: 38926609
doi: 10.1038/s42003-024-06469-y
pii: 10.1038/s42003-024-06469-y
doi:
Substances chimiques
Phosphates
0
Bacterial Proteins
0
Phosphate Transport Proteins
0
PhoR protein, Bacteria
107121-16-4
PhoB protein, Bacteria
104220-36-2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
772Subventions
Organisme : Max-Planck-Gesellschaft (Max Planck Society)
ID : Max Planck Fellowship
Informations de copyright
© 2024. The Author(s).
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