Analysis of CFTR mRNA and Protein in Peripheral Blood Mononuclear Cells via Quantitative Real-Time PCR and Western Blot.

Cystic Fibrosis Transmembrane Conductance Regulator / genetics Humans RNA, Messenger / genetics Leukocytes, Mononuclear / metabolism Blotting, Western Real-Time Polymerase Chain Reaction / methods Cystic Fibrosis / metabolism Killer Cells, Natural / metabolism Flow Cytometry / methods CD4-Positive T-Lymphocytes / metabolism
CFTR RNA Western blot cystic fibrosis expression immune cell lines immune cells leukocytes protein qPCR

Journal

International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791

Informations de publication

Date de publication:
08 Jun 2024
Historique:
received: 21 12 2023
revised: 31 05 2024
accepted: 06 06 2024
medline: 27 6 2024
pubmed: 27 6 2024
entrez: 27 6 2024
Statut: epublish

Résumé

The

Identifiants

DOI: 10.3390/ijms25126367 PMID: 38928073
pubmed: 38928073
pii: ijms25126367
doi: 10.3390/ijms25126367
pii:
doi:

Substances chimiques

Cystic Fibrosis Transmembrane Conductance Regulator 126880-72-6
RNA, Messenger 0
CFTR protein, human 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : IZKF Erlangen
ID : Clinician Scientist Program

Auteurs

Alexander Schnell (A)

Department of Pediatric and Adolescent Medicine, University Hospital Erlangen, 91054 Erlangen, Germany.

Stephanie Tamm (S)

Department of Pediatric Pneumology, Allergology and Neonatology, Hannover Medical School, 30625 Hannover, Germany.
Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), German Center for Lung Research, Hannover Medical School, 30625 Hannover, Germany.

Silke Hedtfeld (S)

Department of Pediatric Pneumology, Allergology and Neonatology, Hannover Medical School, 30625 Hannover, Germany.
Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), German Center for Lung Research, Hannover Medical School, 30625 Hannover, Germany.

Claudio Rodriguez Gonzalez (C)

Department of Pediatric Pneumology, Allergology and Neonatology, Hannover Medical School, 30625 Hannover, Germany.

Andre Hoerning (A)

Department of Pediatric and Adolescent Medicine, University Hospital Erlangen, 91054 Erlangen, Germany.
ORCID: 0000-0001-6241-7168

Nico Lachmann (N)

Department of Pediatric Pneumology, Allergology and Neonatology, Hannover Medical School, 30625 Hannover, Germany.
Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), German Center for Lung Research, Hannover Medical School, 30625 Hannover, Germany.
Fraunhofer Institute for Toxicology and Experimental Medicine, 30625 Hannover, Germany.
ORCID: 0000-0002-4245-1497

Frauke Stanke (F)

Department of Pediatric Pneumology, Allergology and Neonatology, Hannover Medical School, 30625 Hannover, Germany.
Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), German Center for Lung Research, Hannover Medical School, 30625 Hannover, Germany.
ORCID: 0000-0002-6186-0149

Anna-Maria Dittrich (AM)

Department of Pediatric Pneumology, Allergology and Neonatology, Hannover Medical School, 30625 Hannover, Germany.
Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), German Center for Lung Research, Hannover Medical School, 30625 Hannover, Germany.

Antje Munder (A)

Department of Pediatric Pneumology, Allergology and Neonatology, Hannover Medical School, 30625 Hannover, Germany.
Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), German Center for Lung Research, Hannover Medical School, 30625 Hannover, Germany.
ORCID: 0000-0002-3198-8336

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Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Protéines de transport membranaire › Pompes ioniques › Transporteurs d'anions › Transporteurs d'anions organiques › Transporteurs d'anions organiques ATP-dépendants › Protéines associées à la multirésistance aux médicaments › Protéine CFTR › Analysis of CFTR mRNA and Protein in...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Analysis of CFTR mRNA and Protein in...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN messager › Analysis of CFTR mRNA and Protein in...
questionsmedicales.fr › Systèmes sanguin et immunitaire › Système immunitaire › Leucocytes › Agranulocytes › Analysis of CFTR mRNA and Protein in...
questionsmedicales.fr › Techniques d'investigation › Techniques de sonde moléculaire › Dosage immunologique › Immunotransfert › Technique de Western › Analysis of CFTR mRNA and Protein in...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Techniques d'amplification d'acides nucléiques › Réaction de polymérisation en chaîne › Réaction de polymérisation en chaine en temps réel › Analysis of CFTR mRNA and Protein in...
questionsmedicales.fr › Malformations et maladies congénitales, héréditaires et néonatales › Maladies néonatales › Mucoviscidose › Analysis of CFTR mRNA and Protein in...
questionsmedicales.fr › Systèmes sanguin et immunitaire › Système immunitaire › Leucocytes › Agranulocytes › Lymphocytes › Cellules tueuses naturelles › Analysis of CFTR mRNA and Protein in...
questionsmedicales.fr › Techniques d'investigation › Techniques cytologiques › Cytophotométrie › Cytométrie en flux › Analysis of CFTR mRNA and Protein in...
questionsmedicales.fr › Systèmes sanguin et immunitaire › Système immunitaire › Leucocytes › Agranulocytes › Lymphocytes › Lymphocytes T › Lymphocytes T CD4+ › Analysis of CFTR mRNA and Protein in...