High-throughput combination assay for studying biofilm formation of uropathogenic Escherichia coli.
Assay optimization
Biofilm
Crystal violet
Resazurin
Screening
UPEC
Journal
Archives of microbiology
ISSN: 1432-072X
Titre abrégé: Arch Microbiol
Pays: Germany
ID NLM: 0410427
Informations de publication
Date de publication:
05 Jul 2024
05 Jul 2024
Historique:
received:
23
05
2024
accepted:
03
06
2024
revised:
23
05
2024
medline:
5
7
2024
pubmed:
5
7
2024
entrez:
5
7
2024
Statut:
epublish
Résumé
Uropathogenic Escherichia coli, the most common cause for urinary tract infections, forms biofilm enhancing its antibiotic resistance. To assess the effects of compounds on biofilm formation of uropathogenic Escherichia coli UMN026 strain, a high-throughput combination assay using resazurin followed by crystal violet staining was optimized for 384-well microplate. Optimized assay parameters included, for example, resazurin and crystal violet concentrations, and incubation time for readouts. For the assay validation, quality parameters Z' factor, coefficient of variation, signal-to-noise, and signal-to-background were calculated. Microplate uniformity, signal variability, edge well effects, and fold shift were also assessed. Finally, a screening with known antibacterial compounds was conducted to evaluate the assay performance. The best conditions found were achieved by using 12 µg/mL resazurin for 150 min and 0.023% crystal violet. This assay was able to detect compounds displaying antibiofilm activity against UMN026 strain at sub-inhibitory concentrations, in terms of metabolic activity and/or biomass.
Identifiants
pubmed: 38967798
doi: 10.1007/s00203-024-04029-w
pii: 10.1007/s00203-024-04029-w
doi:
Substances chimiques
Xanthenes
0
resazurin
1FN9YD6968
Anti-Bacterial Agents
0
Gentian Violet
J4Z741D6O5
Oxazines
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
344Informations de copyright
© 2024. The Author(s).
Références
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