Fitness costs of Tn1546-type transposons harboring the vanA operon by plasmid type and structural diversity in Enterococcus faecium.
Plasmids
/ genetics
DNA Transposable Elements
Enterococcus faecium
/ genetics
Operon
Humans
Bacterial Proteins
/ genetics
Republic of Korea
Carbon-Oxygen Ligases
/ genetics
Microbial Sensitivity Tests
Anti-Bacterial Agents
/ pharmacology
Whole Genome Sequencing
Gram-Positive Bacterial Infections
/ microbiology
Vancomycin-Resistant Enterococci
/ genetics
Vancomycin Resistance
/ genetics
Genetic Fitness
Vancomycin
/ pharmacology
Conjugation, Genetic
Enterococcus faecium
Fitness cost
Teicoplanin
Tn1546
Vancomycin
Journal
Annals of clinical microbiology and antimicrobials
ISSN: 1476-0711
Titre abrégé: Ann Clin Microbiol Antimicrob
Pays: England
ID NLM: 101152152
Informations de publication
Date de publication:
08 Jul 2024
08 Jul 2024
Historique:
received:
15
03
2024
accepted:
28
06
2024
medline:
9
7
2024
pubmed:
9
7
2024
entrez:
8
7
2024
Statut:
epublish
Résumé
This study analyzed the genetic traits and fitness costs of vancomycin-resistant Enterococcus faecium (VREfm) blood isolates carrying Tn1546-type transposons harboring the vanA operon. All E. faecium blood isolates were collected from eight general hospitals in South Korea during one-year study period. Antimicrobial susceptibility testing and vanA and vanB PCR were performed. Growth rates of E. faecium isolates were determined. The vanA-positive isolates were subjected to whole genome sequencing and conjugation experiments. Among 308 E. faecium isolates, 132 (42.9%) were positive for vanA. All Tn1546-type transposons harboring the vanA operon located on the plasmids, but on the chromosome in seven isolates. The plasmids harboring the vanA operon were grouped into four types; two types of circular, nonconjugative plasmids (Type A, n = 50; Type B, n = 46), and two types of putative linear, conjugative plasmids (Type C, n = 16; Type D, n = 5). Growth rates of vanA-positive E. faecium isolates were significantly lower than those of vanA-negative isolates (P < 0.001), and reduction in growth rate under vancomycin pressure was significantly larger in isolates harboring putative linear plasmids than in those harboring circular plasmids (P = 0.020). The possession of vanA operon was costly to bacterial hosts in antimicrobial-free environment, which provide evidence for the importance of reducing vancomycin pressure for prevention of VREfm dissemination. Fitness burden to bacterial hosts was varied by type and size of the vanA operon-harboring plasmid.
Sections du résumé
BACKGROUND
BACKGROUND
This study analyzed the genetic traits and fitness costs of vancomycin-resistant Enterococcus faecium (VREfm) blood isolates carrying Tn1546-type transposons harboring the vanA operon.
METHODS
METHODS
All E. faecium blood isolates were collected from eight general hospitals in South Korea during one-year study period. Antimicrobial susceptibility testing and vanA and vanB PCR were performed. Growth rates of E. faecium isolates were determined. The vanA-positive isolates were subjected to whole genome sequencing and conjugation experiments.
RESULTS
RESULTS
Among 308 E. faecium isolates, 132 (42.9%) were positive for vanA. All Tn1546-type transposons harboring the vanA operon located on the plasmids, but on the chromosome in seven isolates. The plasmids harboring the vanA operon were grouped into four types; two types of circular, nonconjugative plasmids (Type A, n = 50; Type B, n = 46), and two types of putative linear, conjugative plasmids (Type C, n = 16; Type D, n = 5). Growth rates of vanA-positive E. faecium isolates were significantly lower than those of vanA-negative isolates (P < 0.001), and reduction in growth rate under vancomycin pressure was significantly larger in isolates harboring putative linear plasmids than in those harboring circular plasmids (P = 0.020).
CONCLUSIONS
CONCLUSIONS
The possession of vanA operon was costly to bacterial hosts in antimicrobial-free environment, which provide evidence for the importance of reducing vancomycin pressure for prevention of VREfm dissemination. Fitness burden to bacterial hosts was varied by type and size of the vanA operon-harboring plasmid.
Identifiants
pubmed: 38978096
doi: 10.1186/s12941-024-00722-2
pii: 10.1186/s12941-024-00722-2
doi:
Substances chimiques
DNA Transposable Elements
0
Bacterial Proteins
0
VanA ligase, Bacteria
0
Carbon-Oxygen Ligases
EC 6.1.-
Anti-Bacterial Agents
0
Vancomycin
6Q205EH1VU
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
62Subventions
Organisme : Korea Disease Control and Prevention Agency
ID : 2023-10-001
Organisme : Korea Disease Control and Prevention Agency
ID : 2023-10-002
Organisme : Korea Disease Control and Prevention Agency
ID : 2017E4400102
Informations de copyright
© 2024. The Author(s).
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