Investigating the impact of 2-OHOA-embedded liposomes on biophysical properties of cancer cell membranes via Laurdan two-photon microscopy imaging.
Liposomes
/ chemistry
Humans
Cell Membrane
/ metabolism
Membrane Fluidity
/ drug effects
2-Naphthylamine
/ analogs & derivatives
Apoptosis
/ drug effects
Laurates
/ chemistry
Microscopy, Fluorescence, Multiphoton
Cell Line, Tumor
Neoplasms
/ drug therapy
Antineoplastic Agents
/ pharmacology
Oleic Acids
/ chemistry
Fluorescent Dyes
/ chemistry
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
09 Jul 2024
09 Jul 2024
Historique:
received:
13
02
2024
accepted:
24
06
2024
medline:
10
7
2024
pubmed:
10
7
2024
entrez:
9
7
2024
Statut:
epublish
Résumé
2-Hydroxyoleic acid (2-OHOA) has gained attention as a membrane lipid therapy (MLT) anti-cancer drug. However, in the viewpoint of anti-cancer drug, 2-OHOA shows poor water solubility and its effectiveness still has space for improvement. Thus, this study aimed to overcome the problems by formulating 2-OHOA into liposome dosage form. Furthermore, in the context of MLT reagents, the influence of 2-OHOA on the biophysical properties of the cytoplasmic membrane remains largely unexplored. To bridge this gap, our study specifically focused the alterations in cancer cell membrane fluidity and lipid packing characteristics before and after treatment. By using a two-photon microscope and the Laurdan fluorescence probe, we noted that liposomes incorporating 2-OHOA induced a more significant reduction in cancer cell membrane fluidity, accompanied by a heightened rate of cellular apoptosis when compared to the non-formulated 2-OHOA. Importantly, the enhanced efficacy of 2-OHOA within the liposomal formulation demonstrated a correlation with its endocytic uptake mechanism. In conclusion, our findings underscore the significant influence of 2-OHOA on the biophysical properties of cancer plasma membranes, emphasizing the potential of liposomes as an optimized delivery system for 2-OHOA in anti-cancer therapy.
Identifiants
pubmed: 38982188
doi: 10.1038/s41598-024-65812-9
pii: 10.1038/s41598-024-65812-9
doi:
Substances chimiques
Liposomes
0
laurdan
Y97FBL93VW
2-Naphthylamine
CKR7XL41N4
Laurates
0
Antineoplastic Agents
0
Oleic Acids
0
Fluorescent Dyes
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
15831Informations de copyright
© 2024. The Author(s).
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