Fast-track adaptive laboratory evolution of Cupriavidus necator H16 with divalent metal cations.
Cupriavidus necator H16
Ralstonia eutropha H16
adaptive laboratory evolution (ALE)
divalent metal cations
glycerol utilization
Journal
Biotechnology journal
ISSN: 1860-7314
Titre abrégé: Biotechnol J
Pays: Germany
ID NLM: 101265833
Informations de publication
Date de publication:
Jul 2024
Jul 2024
Historique:
revised:
05
06
2024
received:
27
10
2023
accepted:
12
06
2024
medline:
11
7
2024
pubmed:
11
7
2024
entrez:
10
7
2024
Statut:
ppublish
Résumé
Microbial strain improvement through adaptive laboratory evolution (ALE) has been a key strategy in biotechnology for enhancing desired phenotypic traits. In this Biotech Method paper, we present an accelerated ALE (aALE) workflow and its successful implementation in evolving Cupriavidus necator H16 for enhanced tolerance toward elevated glycerol concentrations. The method involves the deliberate induction of genetic diversity through controlled exposure to divalent metal cations, enabling the rapid identification of improved variants. Through this approach, we observed the emergence of robust variants capable of growing in high glycerol concentration environments, demonstrating the efficacy of our aALE workflow. When cultivated in 10% v/v glycerol, the adapted variant Mn-C2-B11, selected through aALE, achieved a final OD
Identifiants
pubmed: 38987216
doi: 10.1002/biot.202300577
doi:
Substances chimiques
Glycerol
PDC6A3C0OX
Cations, Divalent
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2300577Subventions
Organisme : National Research Council of Thailand
ID : P2250317/3
Organisme : Engineering and Physical Sciences Research Council
ID : EP/X025853/1
Organisme : Royal Academy of Engineering
ID : LTSRF1819∖15∖21
Informations de copyright
© 2024 The Author(s). Biotechnology Journal published by Wiley‐VCH GmbH.
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