FlhE functions as a chaperone to prevent formation of periplasmic flagella in Gram-negative bacteria.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
14 Jul 2024
14 Jul 2024
Historique:
received:
25
03
2024
accepted:
04
07
2024
medline:
15
7
2024
pubmed:
15
7
2024
entrez:
14
7
2024
Statut:
epublish
Résumé
The bacterial flagellum, which facilitates motility, is composed of ~20 structural proteins organized into a long extracellular filament connected to a cytoplasmic rotor-stator complex via a periplasmic rod. Flagellum assembly is regulated by multiple checkpoints that ensure an ordered gene expression pattern coupled to the assembly of the various building blocks. Here, we use epifluorescence, super-resolution, and transmission electron microscopy to show that the absence of a periplasmic protein (FlhE) prevents proper flagellar morphogenesis and results in the formation of periplasmic flagella in Salmonella enterica. The periplasmic flagella disrupt cell wall synthesis, leading to a loss of normal cell morphology resulting in cell lysis. We propose that FlhE functions as a periplasmic chaperone to control assembly of the periplasmic rod, thus preventing formation of periplasmic flagella.
Identifiants
pubmed: 39004688
doi: 10.1038/s41467-024-50278-0
pii: 10.1038/s41467-024-50278-0
doi:
Substances chimiques
Molecular Chaperones
0
Bacterial Proteins
0
Periplasmic Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
5921Subventions
Organisme : EC | EU Framework Programme for Research and Innovation H2020 | H2020 Priority Excellent Science | H2020 European Research Council (H2020 Excellent Science - European Research Council)
ID : agreement no. 864971
Informations de copyright
© 2024. The Author(s).
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