The identification of high-performing antibodies for Sequestosome-1 for use in Western blot, immunoprecipitation and immunofluorescence.

Sequestosome-1 Protein / immunology Humans Fluorescent Antibody Technique / methods Immunoprecipitation / methods Blotting, Western Antibodies / immunology
SQSTM1 Sequestosome-1 Uniprot ID Q13501 Western blot antibody characterization antibody validation immunofluorescence immunoprecipitation

Journal

F1000Research
ISSN: 2046-1402
Titre abrégé: F1000Res
Pays: England
ID NLM: 101594320

Informations de publication

Date de publication:
2023
Historique:
accepted: 08 07 2024
medline: 15 7 2024
pubmed: 15 7 2024
entrez: 15 7 2024
Statut: epublish

Résumé

Sequestosome-1, encoded by the gene

Identifiants

DOI: 10.12688/f1000research.132628.2 PMID: 39006307 PMC: PMC11240082
pubmed: 39006307
doi: 10.12688/f1000research.132628.2
pmc: PMC11240082
doi:

Substances chimiques

Sequestosome-1 Protein 0
Antibodies 0
SQSTM1 protein, human 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

324

Informations de copyright

Copyright: © 2024 Ayoubi R et al.

Déclaration de conflit d'intérêts

Competing interests: For this project, the laboratory of Peter McPherson developed partnerships with high-quality antibody manufacturers and knockout cell line providers. The partners provide antibodies and knockout cell lines to the McPherson laboratory at no cost. These partners include: - Abcam- ABclonal -Aviva Systems Biology -Bio Techne -Cell Signalling Technology -Developmental Studies Hybridoma Bank -GeneTex – Horizon Discovery – Proteintech – Synaptic Systems –Thermo Fisher Scientific.

Auteurs

Riham Ayoubi (R)

Department of Neurology and Neurosurgery, Structural Genomics Consortium, The Montreal Neurological Institute, McGill University, Montreal, Québec, H3A 2B4, Canada.

Walaa Alshafie (W)

Department of Neurology and Neurosurgery, Structural Genomics Consortium, The Montreal Neurological Institute, McGill University, Montreal, Québec, H3A 2B4, Canada.

Irina Shlaifer (I)

The Neuro's Early Drug Discovery Unit (EDDU), Structural Genomics Consortium, McGill University, Montreal, Québec, H3A 2B4, Canada.

Kathleen Southern (K)

Department of Neurology and Neurosurgery, Structural Genomics Consortium, The Montreal Neurological Institute, McGill University, Montreal, Québec, H3A 2B4, Canada.
ORCID: 0000-0002-4125-3608

Peter S McPherson (PS)

Department of Neurology and Neurosurgery, Structural Genomics Consortium, The Montreal Neurological Institute, McGill University, Montreal, Québec, H3A 2B4, Canada.

Carl Laflamme (C)

Department of Neurology and Neurosurgery, Structural Genomics Consortium, The Montreal Neurological Institute, McGill University, Montreal, Québec, H3A 2B4, Canada.
ORCID: 0000-0001-5906-025X

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Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines et peptides de signalisation intracellulaire › Protéines adaptatrices de la transduction du signal › Séquestosome-1 › The identification of high-performing...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › The identification of high-performing...
questionsmedicales.fr › Techniques d'investigation › Techniques immunologiques › Technique d'immunofluorescence › The identification of high-performing...
questionsmedicales.fr › Techniques d'investigation › Techniques immunologiques › Immunoprécipitation › The identification of high-performing...
questionsmedicales.fr › Techniques d'investigation › Techniques de sonde moléculaire › Dosage immunologique › Immunotransfert › Technique de Western › The identification of high-performing...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Globulines › Sérum-globulines › Immunoglobulines › Anticorps › The identification of high-performing...