Characterization of a thermostable protease from Bacillus subtilis BSP strain.
Bacillus subtilis BSP
Detergent/organic solvent-tolerant
Response surface methodology
Statistical optimization
Journal
BMC biotechnology
ISSN: 1472-6750
Titre abrégé: BMC Biotechnol
Pays: England
ID NLM: 101088663
Informations de publication
Date de publication:
15 Jul 2024
15 Jul 2024
Historique:
received:
28
03
2024
accepted:
20
06
2024
medline:
16
7
2024
pubmed:
16
7
2024
entrez:
15
7
2024
Statut:
epublish
Résumé
This study used conservative one variable-at-a-time study and statistical surface response methods to increase the yields of an extracellular thermostable protease secreted by a newly identified thermophilic Bacillus subtilis BSP strain. Using conventional optimization techniques, physical parameters in submerged fermentation were adjusted at the shake flask level to reach 184 U/mL. These physicochemical parameters were further optimized by statistical surface response methodology using Box Behnken design, and the protease yield increased to 295 U/mL. The protease was purified and characterized biochemically. Both Ca
Identifiants
pubmed: 39010004
doi: 10.1186/s12896-024-00870-5
pii: 10.1186/s12896-024-00870-5
doi:
Substances chimiques
Bacterial Proteins
0
Hydrogen Peroxide
BBX060AN9V
Peptide Hydrolases
EC 3.4.-
Solvents
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
49Informations de copyright
© 2024. The Author(s).
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