Investigations of microbiota composition and neuroactive pathways in association with symptoms of stress and depression in a cohort of healthy women.
Humans
Female
Adult
Young Adult
Cross-Sectional Studies
Gastrointestinal Microbiome
Adolescent
Depression
/ microbiology
Vagina
/ microbiology
Feces
/ microbiology
Stress, Psychological
/ microbiology
Microbiota
Denmark
Healthy Volunteers
Brain-Gut Axis
/ physiology
Surveys and Questionnaires
Metagenomics
/ methods
Bacteria
/ classification
(microbiota-)gut-brain axis
depressive symptoms
diet
microbiome
perceived stress
proteobacteria
shotgun sequencing
tryptophan
Journal
Frontiers in cellular and infection microbiology
ISSN: 2235-2988
Titre abrégé: Front Cell Infect Microbiol
Pays: Switzerland
ID NLM: 101585359
Informations de publication
Date de publication:
2024
2024
Historique:
received:
23
10
2023
accepted:
07
06
2024
medline:
17
7
2024
pubmed:
17
7
2024
entrez:
17
7
2024
Statut:
epublish
Résumé
Despite mounting evidence of gut-brain involvement in psychiatric conditions, functional data remain limited, and analyses of other microbial niches, such as the vaginal microbiota, are lacking in relation to mental health. This aim of this study was to investigate if the connections between the gut microbiome and mental health observed in populations with a clinical diagnosis of mental illness extend to healthy women experiencing stress and depressive symptoms. Additionally, this study examined the functional pathways of the gut microbiota according to the levels of psychological symptoms. Furthermore, the study aimed to explore potential correlations between the vaginal microbiome and mental health parameters in young women without psychiatric diagnoses. In this cross-sectional study, 160 healthy Danish women (aged 18-40 years) filled out questionnaires with validated scales measuring symptoms of stress and depression and frequency of dietary intake. Fecal and vaginal microbiota samples were collected at the beginning of the menstrual cycle and vaginal samples were also collected at cycle day 8-12 and 18-22. Shotgun metagenomic profiling of the gut and vaginal microbiome was performed. The Kyoto Encyclopedia of Genes and Genomes (KEGG) was used for functional profiling and 56 Gut Brain Modules were analyzed in the fecal samples. The relative abundance in the gut of the genera The findings of this study support the concept of a microbiota-associated effect on the neuroactive pathways even in healthy young women. This suggest, that targeting the gut microbiome could be a promising approach for future psychiatric interventions.
Sections du résumé
Background
UNASSIGNED
Despite mounting evidence of gut-brain involvement in psychiatric conditions, functional data remain limited, and analyses of other microbial niches, such as the vaginal microbiota, are lacking in relation to mental health. This aim of this study was to investigate if the connections between the gut microbiome and mental health observed in populations with a clinical diagnosis of mental illness extend to healthy women experiencing stress and depressive symptoms. Additionally, this study examined the functional pathways of the gut microbiota according to the levels of psychological symptoms. Furthermore, the study aimed to explore potential correlations between the vaginal microbiome and mental health parameters in young women without psychiatric diagnoses.
Methods
UNASSIGNED
In this cross-sectional study, 160 healthy Danish women (aged 18-40 years) filled out questionnaires with validated scales measuring symptoms of stress and depression and frequency of dietary intake. Fecal and vaginal microbiota samples were collected at the beginning of the menstrual cycle and vaginal samples were also collected at cycle day 8-12 and 18-22. Shotgun metagenomic profiling of the gut and vaginal microbiome was performed. The Kyoto Encyclopedia of Genes and Genomes (KEGG) was used for functional profiling and 56 Gut Brain Modules were analyzed in the fecal samples.
Results
UNASSIGNED
The relative abundance in the gut of the genera
Conclusions
UNASSIGNED
The findings of this study support the concept of a microbiota-associated effect on the neuroactive pathways even in healthy young women. This suggest, that targeting the gut microbiome could be a promising approach for future psychiatric interventions.
Identifiants
pubmed: 39015337
doi: 10.3389/fcimb.2024.1324794
pmc: PMC11249552
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1324794Informations de copyright
Copyright © 2024 Bashir, Hugerth, Krog, Prast-Nielsen, Edfeldt, Boulund, Schacht, Tetens, Engstrand, Schuppe-Koistinen, Fransson and Nielsen.
Déclaration de conflit d'intérêts
An unrestricted research grant from Ferring Pharmaceuticals enabled the clinical infrastructure, and sampling as well as the analyses at the Centre for Translational Microbiome Research. The funder was not involved in the study design, collection, analysis, interpretation of data, the writing of this article or the decision to submit it for publication.