Modeling the liquid-liquid chromatography separation of cannabinoids from hemp extracts.
Cannabis sativa
cannabidiol
centrifugal partition chromatography
countercurrent chromatography
nonlinear distribution equilibria
Journal
Journal of separation science
ISSN: 1615-9314
Titre abrégé: J Sep Sci
Pays: Germany
ID NLM: 101088554
Informations de publication
Date de publication:
Jun 2024
Jun 2024
Historique:
revised:
19
05
2024
received:
28
03
2024
accepted:
21
05
2024
medline:
20
7
2024
pubmed:
20
7
2024
entrez:
20
7
2024
Statut:
ppublish
Résumé
The separation of cannabinoids from hemp materials is nowadays one of the most promising industrial applications of liquid-liquid chromatography (LLC). Despite various experimental research efforts to purify cannabinoids, there are currently few works on process modeling. Thus, this study aimed to explore a straightforward approach to model the LLC separation of cannabinoids from two hemp extracts with different compositions. The feed materials were simplified to mixtures of preselected key components (i.e., cannabidiol, tetrahydrocannabinol, cannabigerol, and cannabinol). The elution profiles of cannabinoids were simulated using the equilibrium-cell model with an empirical nonlinear correlation. The model parameters were derived from the elution profiles of single-solute pulse injections. For the validation of the proposed approach, LLC separations with the two hemp extracts were performed in descending mode with the solvent system composed of hexane/methanol/water 10/8/2 (v/v/v). The injected sample concentrations were gradually increased from 5 to 100 mg/mL. The results showed that the approach could describe reasonably well the elution behavior of the cannabinoids, with deviations of only 1-2 min between simulated and experimental elution times. However, to improve the prediction accuracy, the model parameters can be refitted to the elution profiles of 3-4 systematically selected pulse injections with specific hemp extracts.
Identifiants
pubmed: 39031845
doi: 10.1002/jssc.202400239
doi:
Substances chimiques
Cannabinoids
0
Plant Extracts
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2400239Subventions
Organisme : Bundesministerium für Wirtschaft und Klimaschutz
ID : ZIM no.
Organisme : Bundesministerium für Wirtschaft und Klimaschutz
ID : 16KN092721
Informations de copyright
© 2024 The Author(s). Journal of Separation Science published by Wiley‐VCH GmbH.
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