Infection of mice by the enteroaggregative E. coli strain 042 and two mutant derivatives overexpressing virulence factors: impact on disease markers, gut microbiota and concentration of SCFAs in feces.
Animals
Virulence Factors
/ genetics
Escherichia coli Infections
/ microbiology
Mice
Feces
/ microbiology
Escherichia coli
/ pathogenicity
Gastrointestinal Microbiome
Escherichia coli Proteins
/ genetics
Fatty Acids, Volatile
/ metabolism
Mutation
Virulence
/ genetics
Female
Disease Models, Animal
Gene Expression Regulation, Bacterial
Biomarkers
Trans-Activators
AggR
Enteroaggregative E. coli
Infection
Mice
Microbiota
SCFAs
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
23 Jul 2024
23 Jul 2024
Historique:
received:
16
04
2024
accepted:
15
07
2024
medline:
24
7
2024
pubmed:
24
7
2024
entrez:
23
7
2024
Statut:
epublish
Résumé
Several pathogenic Escherichia coli strains cause diarrhea. Enteroaggregative E. coli (EAEC) strains are one of the diarrheagenic pathotypes. EAEC cells form a "stacked-brick" arrangement over the intestinal epithelial cells. EAEC isolates express, among other virulence determinants, the AggR transcriptional activator and the aggregative adherence fimbriae (AAF). Overexpression of the aggR gene results in increased expression of virulence factors such as the aff genes, as well as several genes involved in specific metabolic pathways such as fatty acid degradation (fad) and arginine degradation (ast). To support the hypothesis that induction of the expression of some of these pathways may play a role in EAEC virulence, in this study we used a murine infection model to evaluate the impact of the expression of these pathways on infection parameters. Mice infected with a mutant derivative of the EAEC strain 042, characterized by overexpression of the aggR gene, showed increased disease symptoms compared to those exhibited by mice infected with the wild type (wt) strain 042. Several of these symptoms were not increased when the infecting mutant, which overexpressed aggR, lacked the fad and ast pathways. Therefore, our results support the hypothesis that different metabolic pathways contribute to EAEC virulence.
Identifiants
pubmed: 39043759
doi: 10.1038/s41598-024-67731-1
pii: 10.1038/s41598-024-67731-1
doi:
Substances chimiques
Virulence Factors
0
Escherichia coli Proteins
0
Fatty Acids, Volatile
0
AggR protein, E coli
0
Biomarkers
0
Trans-Activators
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
16945Subventions
Organisme : Ministerio de Ciencia, Innovación y Universidades
ID : JDC2022-050269-I
Organisme : Ministerio de Ciencia e Innovación
ID : CEX2021-001189-S/ MCIN/AEI / 10.13039/501100011033
Organisme : Ministerio de Ciencia e Innovación
ID : MICIN/AEI/FEDER (CEX2021-001234-M)
Organisme : Ministerio de Economía y Competitividad
ID : PID2019-107479RB-I00
Organisme : Generalitat de Catalunya
ID : 2021SGR300
Organisme : Generalitat Valenciana
ID : CDEIGENT 2020-02
Informations de copyright
© 2024. The Author(s).
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