Gardnerella Species and Their Association With Bacterial Vaginosis.
Humans
Vaginosis, Bacterial
/ microbiology
Female
Adult
Gardnerella
/ isolation & purification
Young Adult
Vagina
/ microbiology
Washington
/ epidemiology
Gardnerella vaginalis
/ isolation & purification
Gram-Positive Bacterial Infections
/ microbiology
Adolescent
Prevalence
Middle Aged
DNA, Bacterial
/ genetics
Chaperonin 60
/ genetics
Real-Time Polymerase Chain Reaction
Gardnerella
cpn60
bacterial vaginosis
microbiome
Journal
The Journal of infectious diseases
ISSN: 1537-6613
Titre abrégé: J Infect Dis
Pays: United States
ID NLM: 0413675
Informations de publication
Date de publication:
25 Jul 2024
25 Jul 2024
Historique:
received:
22
09
2023
accepted:
22
01
2024
medline:
26
7
2024
pubmed:
26
7
2024
entrez:
25
7
2024
Statut:
ppublish
Résumé
Bacterial vaginosis (BV) is a condition marked by high vaginal bacterial diversity. Gardnerella vaginalis has been implicated in BV but is also detected in healthy women. The Gardnerella genus has been expanded to encompass 6 validly named species and several genomospecies. We hypothesized that particular Gardnerella species may be more associated with BV. Quantitative polymerase chain reaction (PCR) assays were developed targeting the cpn60 gene of species groups including G. vaginalis, G. piotii/pickettii, G. swidsinskii/greenwoodii, and G. leopoldii. These assays were applied to vaginal swabs from individuals with (n = 101) and without BV (n = 150) attending a sexual health clinic in Seattle, Washington. Weekly swabs were collected from 42 participants for up to 12 weeks. Concentrations and prevalence of each Gardnerella species group were significantly higher in participants with BV; 91.1% of BV-positive participants had 3 or more Gardnerella species groups detected compared to 32.0% of BV-negative participants (P < .0001). BV-negative participants with 3 or more species groups detected were more likely to develop BV within 100 days versus those with fewer (60.5% vs 3.7%, P < .0001). These results suggest that BV reflects a state of high Gardnerella species diversity. No Gardnerella species group was a specific marker for BV.
Sections du résumé
BACKGROUND
BACKGROUND
Bacterial vaginosis (BV) is a condition marked by high vaginal bacterial diversity. Gardnerella vaginalis has been implicated in BV but is also detected in healthy women. The Gardnerella genus has been expanded to encompass 6 validly named species and several genomospecies. We hypothesized that particular Gardnerella species may be more associated with BV.
METHODS
METHODS
Quantitative polymerase chain reaction (PCR) assays were developed targeting the cpn60 gene of species groups including G. vaginalis, G. piotii/pickettii, G. swidsinskii/greenwoodii, and G. leopoldii. These assays were applied to vaginal swabs from individuals with (n = 101) and without BV (n = 150) attending a sexual health clinic in Seattle, Washington. Weekly swabs were collected from 42 participants for up to 12 weeks.
RESULTS
RESULTS
Concentrations and prevalence of each Gardnerella species group were significantly higher in participants with BV; 91.1% of BV-positive participants had 3 or more Gardnerella species groups detected compared to 32.0% of BV-negative participants (P < .0001). BV-negative participants with 3 or more species groups detected were more likely to develop BV within 100 days versus those with fewer (60.5% vs 3.7%, P < .0001).
CONCLUSIONS
CONCLUSIONS
These results suggest that BV reflects a state of high Gardnerella species diversity. No Gardnerella species group was a specific marker for BV.
Identifiants
pubmed: 39052736
pii: 7720873
doi: 10.1093/infdis/jiae026
doi:
Substances chimiques
DNA, Bacterial
0
Chaperonin 60
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e171-e181Subventions
Organisme : NIH HHS
ID : R01 AI061628
Pays : United States
Informations de copyright
© The Author(s) 2024. Published by Oxford University Press on behalf of Infectious Diseases Society of America.
Déclaration de conflit d'intérêts
Potential conflicts of interest. D. N. F. and T. L. F. receive a royalty from BD related to detection of bacterial vaginosis-associated bacteria and diagnosis of bacterial vaginosis. All other authors report no potential conflicts. All authors have submitted the ICMJE Form for Disclosure of Potential Conflicts of Interest. Conflicts that the editors consider relevant to the content of the manuscript have been disclosed.