Empowering the on-site detection of nucleic acids by integrating CRISPR and digital signal processing.
Humans
Uterine Cervical Neoplasms
/ diagnosis
Female
Papillomavirus Infections
/ diagnosis
CRISPR-Cas Systems
/ genetics
DNA, Viral
/ genetics
Papillomaviridae
/ genetics
Clustered Regularly Interspaced Short Palindromic Repeats
/ genetics
Signal Processing, Computer-Assisted
Cervix Uteri
/ virology
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
25 Jul 2024
25 Jul 2024
Historique:
received:
29
12
2023
accepted:
15
07
2024
medline:
26
7
2024
pubmed:
26
7
2024
entrez:
25
7
2024
Statut:
epublish
Résumé
Addressing the global disparity in cancer care necessitates the development of rapid and affordable nucleic acid (NA) testing technologies. This need is particularly critical for cervical cancer, where molecular detection of human papillomavirus (HPV) has emerged as an accurate screening method. However, implementing this transition in low- and middle-income countries has been challenging due to the high costs and centralized facilities required for current NA tests. Here, we present CreDiT (CRISPR Enhanced Digital Testing) for on-site NA detection. The CreDiT platform integrates i) a one-pot CRISPR strategy that simultaneously amplifies both target NAs and analytical signals and ii) a robust fluorescent detection based on digital communication (encoding/decoding) technology. These features enable a rapid assay (<35 minutes) in a single streamlined workflow. We demonstrate the sensitive detection of cell-derived HPV DNA targets down to single copies and accurate identification of HPV types in clinical cervical brushing specimens (n = 121).
Identifiants
pubmed: 39054353
doi: 10.1038/s41467-024-50588-3
pii: 10.1038/s41467-024-50588-3
doi:
Substances chimiques
DNA, Viral
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
6271Subventions
Organisme : U.S. Department of Health & Human Services | NIH | National Cancer Institute (NCI)
ID : 5R25CA174650
Informations de copyright
© 2024. The Author(s).
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