Isotope dilution with isotopically labeled biomass: An effective alternative for quantitative metabolomics.

Biomass Metabolomics / methods Carbon Isotopes / chemistry Isotope Labeling Saccharomyces cerevisiae / metabolism Calibration Fermentation
Absolute quantification Internal standardization Isotope dilution Isotopically labeled biomass Liquid chromatography-mass spectrometry Metabolomics

Journal

Analytica chimica acta
ISSN: 1873-4324
Titre abrégé: Anal Chim Acta
Pays: Netherlands
ID NLM: 0370534

Informations de publication

Date de publication:
22 Aug 2024
Historique:
received: 22 03 2024
revised: 04 06 2024
accepted: 23 06 2024
medline: 28 7 2024
pubmed: 28 7 2024
entrez: 27 7 2024
Statut: ppublish

Résumé

State-of-the-art quantitative metabolomics relies on isotope dilution using internal standards (IS) derived from fully Independent reverse isotope dilution experiments using different chromatographic methods over a period of several months, delivered a list of 83 The proposed calibration workflow rationalizes time and standard expenditure and is particularly beneficial for laboratories dealing with wide-target assays and small analysis batches. The present assessment serves as a seminal study for further developments of the concept towards absolute quantification from archive high-resolution MS data of U

Sections du résumé

BACKGROUND BACKGROUND
State-of-the-art quantitative metabolomics relies on isotope dilution using internal standards (IS) derived from fully
RESULTS RESULTS
Independent reverse isotope dilution experiments using different chromatographic methods over a period of several months, delivered a list of 83
SIGNIFICANCE CONCLUSIONS
The proposed calibration workflow rationalizes time and standard expenditure and is particularly beneficial for laboratories dealing with wide-target assays and small analysis batches. The present assessment serves as a seminal study for further developments of the concept towards absolute quantification from archive high-resolution MS data of U

Identifiants

DOI: 10.1016/j.aca.2024.342909 PMID: 39067910
pubmed: 39067910
pii: S0003-2670(24)00710-4
doi: 10.1016/j.aca.2024.342909
pii:
doi:

Substances chimiques

Carbon Isotopes 0
Carbon-13 FDJ0A8596D

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

342909

Informations de copyright

Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of competing interest The authors declare no competing financial interest.

Auteurs

Veronika Fitz (V)

University of Vienna, Faculty of Chemistry, Institute of Analytical Chemistry, Waehringer Str. 38, 1090, Vienna, Austria; University of Vienna, Vienna Doctoral School in Chemistry (DoSChem), Waehringer Str. 42, 1090, Vienna, Austria.

Lisa Panzenboeck (L)

University of Vienna, Faculty of Chemistry, Institute of Analytical Chemistry, Waehringer Str. 38, 1090, Vienna, Austria; University of Vienna, Vienna Doctoral School in Chemistry (DoSChem), Waehringer Str. 42, 1090, Vienna, Austria.

Harald Schoeny (H)

University of Vienna, Faculty of Chemistry, Institute of Analytical Chemistry, Waehringer Str. 38, 1090, Vienna, Austria.

Elisabeth Foels (E)

University of Vienna, Faculty of Chemistry, Institute of Analytical Chemistry, Waehringer Str. 38, 1090, Vienna, Austria; University of Vienna, Vienna Doctoral School in Chemistry (DoSChem), Waehringer Str. 42, 1090, Vienna, Austria.

Gunda Koellensperger (G)

University of Vienna, Faculty of Chemistry, Institute of Analytical Chemistry, Waehringer Str. 38, 1090, Vienna, Austria; Vienna Metabolomics Center (VIME), University of Vienna, Althanstr. 14, 1090, Vienna, Austria; Chemistry Meets Biology, University of Vienna, Althanstr. 14, 1090, Vienna, Austria. Electronic address: gunda.koellensperger@univie.ac.at.

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Classifications MeSH

questionsmedicales.fr › Environnement et santé publique › Environnement › Écosystème › Biomasse › Isotope dilution with isotopically labeled...
questionsmedicales.fr › Disciplines des sciences naturelles › Chimie › Biochimie › Métabolomique › Isotope dilution with isotopically labeled...
questionsmedicales.fr › Produits chimiques inorganiques › Isotopes › Isotopes du carbone › Isotope dilution with isotopically labeled...
questionsmedicales.fr › Techniques d'investigation › Marquage isotopique › Isotope dilution with isotopically labeled...
questionsmedicales.fr › Eucaryotes › Champignons › Levures › Saccharomyces › Saccharomyces cerevisiae › Isotope dilution with isotopically labeled...
questionsmedicales.fr › Techniques d'investigation › Poids et mesures › Calibrage › Isotope dilution with isotopically labeled...
questionsmedicales.fr › Métabolisme › Métabolisme glucidique › Fermentation › Isotope dilution with isotopically labeled...