Mitigating pb toxicity in Sesbania sesban L. through activated charcoal supplementation: a hydroponic study on enhanced phytoremediation.
Charcoal
/ pharmacology
Biodegradation, Environmental
Lead
/ toxicity
Sesbania
/ metabolism
Hydroponics
Soil Pollutants
/ toxicity
Oxidative Stress
/ drug effects
Seedlings
/ drug effects
Reactive Oxygen Species
/ metabolism
Antioxidants
/ metabolism
Chlorophyll
/ metabolism
Malondialdehyde
/ metabolism
Heavy metal
Oxidative stress
Soil contamination
Journal
BMC plant biology
ISSN: 1471-2229
Titre abrégé: BMC Plant Biol
Pays: England
ID NLM: 100967807
Informations de publication
Date de publication:
05 Aug 2024
05 Aug 2024
Historique:
received:
23
02
2024
accepted:
01
08
2024
medline:
5
8
2024
pubmed:
5
8
2024
entrez:
4
8
2024
Statut:
epublish
Résumé
Soil contamination by heavy metals is a critical environmental challenge, with Pb being of particular concern due to its propensity to be readily absorbed and accumulated by plants, despite its lack of essential biological functions or beneficial roles in cellular metabolism. Within the scope of phytoremediation, the use of plants for the decontamination of various environmental matrices, the present study investigated the potential of activated charcoal (AC) to enhance the tolerance and mitigation capacity of S. sesban seedlings when exposed to Pb. The experiment was conducted as a factorial arrangement in a completely randomized design in hydroponic conditions. The S. sesban seedlings were subjected to a gradient of Pb concentrations (0, 0.02, 0.2, 2, and 10 mg/L) within the nutrient solution, alongside two distinct AC treatments (0 and 1% inclusion in the culture media). The study reached its conclusion after 60 days. The seedlings exposed to Pb without AC supplementation indicated an escalation in peroxidase (POX) activity, reactive oxygen species (ROS), and malondialdehyde (MDA) levels, signaling an increase in oxidative stress. Conversely, the incorporation of AC into the treatment regime markedly bolstered the antioxidative defense system, as evidenced by the significant elevation in antioxidant capacity and a concomitant reduction in the biomarkers of oxidative stress (POX, ROS, and MDA). With AC application, a notable improvement was observed in the chlorophyll a, total chlorophyll, and plant fresh and dry biomass. These findings illuminate the role of activated charcoal as a viable adjunct in phytoremediation strategies aimed at ameliorating heavy metal stress in plants.
Sections du résumé
BACKGROUND
BACKGROUND
Soil contamination by heavy metals is a critical environmental challenge, with Pb being of particular concern due to its propensity to be readily absorbed and accumulated by plants, despite its lack of essential biological functions or beneficial roles in cellular metabolism. Within the scope of phytoremediation, the use of plants for the decontamination of various environmental matrices, the present study investigated the potential of activated charcoal (AC) to enhance the tolerance and mitigation capacity of S. sesban seedlings when exposed to Pb. The experiment was conducted as a factorial arrangement in a completely randomized design in hydroponic conditions. The S. sesban seedlings were subjected to a gradient of Pb concentrations (0, 0.02, 0.2, 2, and 10 mg/L) within the nutrient solution, alongside two distinct AC treatments (0 and 1% inclusion in the culture media). The study reached its conclusion after 60 days.
RESULTS
RESULTS
The seedlings exposed to Pb without AC supplementation indicated an escalation in peroxidase (POX) activity, reactive oxygen species (ROS), and malondialdehyde (MDA) levels, signaling an increase in oxidative stress. Conversely, the incorporation of AC into the treatment regime markedly bolstered the antioxidative defense system, as evidenced by the significant elevation in antioxidant capacity and a concomitant reduction in the biomarkers of oxidative stress (POX, ROS, and MDA).
CONCLUSIONS
CONCLUSIONS
With AC application, a notable improvement was observed in the chlorophyll a, total chlorophyll, and plant fresh and dry biomass. These findings illuminate the role of activated charcoal as a viable adjunct in phytoremediation strategies aimed at ameliorating heavy metal stress in plants.
Identifiants
pubmed: 39098900
doi: 10.1186/s12870-024-05478-7
pii: 10.1186/s12870-024-05478-7
doi:
Substances chimiques
Charcoal
16291-96-6
Lead
2P299V784P
Soil Pollutants
0
Reactive Oxygen Species
0
Antioxidants
0
Chlorophyll
1406-65-1
Malondialdehyde
4Y8F71G49Q
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
744Informations de copyright
© 2024. The Author(s).
Références
Engwa GA, Ferdinand PU, Nwalo FN, Unachukwu MN. Mechanism and health effects of heavy metal toxicity in humans. Poisoning in the modern world-new tricks for an old dog. 2019;10:70–90.
Deng G, Yang M, Saleem MH, Rehman M, Fahad S, Yang Y, Elshikh MS, Alkahtani J, Ali S, Khan SM. Nitrogen fertilizer ameliorate the remedial capacity of industrial hemp (Cannabis sativa L.) grown in pb contaminated soil. J Plant Nutr. 2021;44(12):1770–8.
doi: 10.1080/01904167.2021.1881553
Collin S, Baskar A, Geevarghese DM, Ali MN, Bahubali P, Choudhary R, Lvov V, Tovar GI, Senatov F, Koppala S, Swamiappan S. Bioaccumulation of lead (pb) and its effects in plants: a review. J Hazard Mater Lett. 2022;3:100064.
doi: 10.1016/j.hazl.2022.100064
Giannakoula A, Therios I, Chatzissavvidis C. Effect of Pb and copper on photosynthetic apparatus in citrus (Citrus aurantium L.) plants. The role of antioxidants in oxidative damage as a response to heavy metal stress. Plants. 2021;10(1):155.
doi: 10.3390/plants10010155
pubmed: 33466929
pmcid: 7830311
Nedjimi B. Phytoremediation: a sustainable environmental technology for heavy metals decontamination. SN Appl Scie. 2021;3(3):286.
doi: 10.1007/s42452-021-04301-4
Alsafran M, Saleem MH, Al Jabri H, Rizwan M, Usman K. Principles and applicability of integrated remediation strategies for heavy metal Removal/Recovery from contaminated environments. J Plant Growth Regul. 2023;42(6):3419–40.
doi: 10.1007/s00344-022-10803-1
Momin RK, Kadam VB. Determination of soluble extractive of some medicinal plants of genus Sesbania of Marathwada region in Maharashtra. 2012; L1.
McComb J, Hentz S, Miller GS, Begonia M, Begonia G. Effects of Pb on plant growth, pb accumulation and phytochelatin contents of hydroponically-grown Sesbania exaltata. World Environ. 2012;2(3):38–43.
doi: 10.5923/j.env.20120203.04
Deliyanni EA, Kyzas GZ, Triantafyllidis KS, Matis KA. Activated carbons for the removal of heavy metal ions: a systematic review of recent literature focused on pb and arsenic ions. Open Chem. 2015;13(1):000010151520150087.
doi: 10.1515/chem-2015-0087
Wang B, Lan J, Bo C, Gong B, Ou J. Adsorption of heavy metal onto biomass-derived activated carbon. RSC Adv. 2023;13(7):4275–302.
doi: 10.1039/D2RA07911A
pubmed: 36760304
pmcid: 9891085
Hassler JW. Activated Carbon. N.Y.: Chem Pub Comp Inc; 1993.
Hassler JW. Purification with Activated Carbon. Chem Pub Comp. NY.1974;169.
Mattson JS, Mark HH. Activated Carbon. N.Y.: Marcel Dekker. Inc.; 1971.
Mahangade AS, Phatak AA. Activated carbon black: versatile adsorbent for the healthcare industry. Pharma Times. 2022;54(08–09):20.
Rizwan M, Ali S, Adrees M, Ibrahim M, Tsang D, Zia-ur-Rehman M, Zahir ZA, Rinklebe J, Tack F, Sik Ok Y. A critical review on effects, tolerance mechanisms and management of cadmium in vegetables. Chemosphere. 2017;182:90–105.
doi: 10.1016/j.chemosphere.2017.05.013
pubmed: 28494365
Biederman LA, Harpole WS. Biochar and its effects on plant productivity and nutrient cycling: a meta-analysis. GCB Bioen. 2013;5(2):202–14.
doi: 10.1111/gcbb.12037
Nolan NE, Kulmatiski A, Beard KH, Norton JM. Activated carbon decreases invasive plant growth by mediating plant–microbe interactions. AoB Plants. 2015;7:plu072.
doi: 10.1093/aobpla/plu072
Mishra S, Srivastava S, Tripathi RD, Kumar R, Seth CS, Gupta DK. Pb detoxification by coontail (Ceratophyllum demersum L.) involves induction of phytochelatins and antioxidant system in response to its accumulation. Chemosphere. 2006;65(6):1027–39.
doi: 10.1016/j.chemosphere.2006.03.033
pubmed: 16682069
Malar S, Shivendra Vikram S, Favas JC, Perumal P. Pb heavy metal toxicity induced changes on growth and antioxidative enzymes level in water hyacinths [Eichhornia crassipes (Mart)]. Botani Stud. 2016;55(1):1–11.
doi: 10.1186/s40529-014-0054-6
Çimrin KM, Turan M, Kapur B. Effect of elemental sulphur on heavy metals solubility and remediation by plants in calcareous soils. Fresen Environ Bull. 2007;16(9):1113–20.
Ghotbi-Ravandi AA, Ghaderian SM, Azizollahi Z. Pb uptake, bioaccumulation and tolerance in summer savory (Satureja hortensis L). J Plant Proc Func. 2019;8(31):63–75.
Kulmatiski A. Changing soils to manage plant communities: activated carbon as a restoration tool in ex-arable fields. Restor Ecol. 2011;101:102–10.
doi: 10.1111/j.1526-100X.2009.00632.x
Del Fabbro C, Güsewell S, Prati D. Allelopathic effects of three plant invaders on germination of native species: a field study. Biol Invasions. 2014;1035–42.
Methela NJ, Islam MS, Lee DS, Yun BW, Mun BG. S-Nitrosoglutathione (GSNO)-Mediated pb detoxification in soybean through the regulation of ROS and Metal-related transcripts. Int J Molecul Sci. 2023;24(12):9901.
doi: 10.3390/ijms24129901
Gopal R, Rizvi AH. Excess pb alters growth, metabolism and translocation of certain nutrients in radish. Chemosphere. 2008;70(9):1539–44.
doi: 10.1016/j.chemosphere.2007.08.043
pubmed: 17923149
Rasouli F, Hassanpouraghdam MB, Pirsarandib Y, Aazami MA, Asadi M, Ercisli S, Mehrabani LV, Puglisi I, Baglieri A. Improvements in the biochemical responses and pb and ni phytoremediation of lavender (Lavandula angustifolia L.) plants through Funneliformis mosseae inoculation. BMC Plant Biol. 2023;23(1):252.
doi: 10.1186/s12870-023-04265-0
pubmed: 37173650
pmcid: 10182630
Huang H, Ullah F, Zhou DX, Yi M, Zhao Y. Mechanisms of ROS regulation of plant development and stress responses. Front Plant Sci. 2019;10:800.
doi: 10.3389/fpls.2019.00800
pubmed: 31293607
pmcid: 6603150
Nadarajah KK. ROS homeostasis in abiotic stress tolerance in plants. Int J Molecul Sci. 2020;21(15):5208.
doi: 10.3390/ijms21155208
Sachdev S, Ansari SA, Ansari MI, Fujita M, Hasanuzzaman M. Abiotic stress and reactive oxygen species: Generation, signaling, and defense mechanisms. Antioxidants. 2021;10(2):277.
doi: 10.3390/antiox10020277
pubmed: 33670123
pmcid: 7916865
Iqbal N, Tanzeem-ul-Haq HS, Turan V, Iqbal M. Soil amendments and foliar melatonin reduced pb uptake, and oxidative stress, and improved spinach quality in Pb-contaminat soil. Plants. 2023;12(9):1829.
doi: 10.3390/plants12091829
pubmed: 37176896
pmcid: 10180591
Rahi AA, Younis U, Ahmed N, Ali MA, Fahad S, Sultan H, Zarei T, Danish S, Taban S, El Enshasy HA, Tamunaidu P. Toxicity of Cadmium and nickel in the context of applied activated carbon biochar for improvement in soil fertility. Saudi J Biol Sci. 2022;29(2):743–50.
doi: 10.1016/j.sjbs.2021.09.035
pubmed: 35197740
Sultan H, Ahmed N, Mubashir M, Danish S. Chemical production of acidified activated carbon and its influences on soil fertility comparative to thermo-pyrolyzed biochar. Sci Rep. 2020;10(1):595.
doi: 10.1038/s41598-020-57535-4
pubmed: 31953498
pmcid: 6969043
Cao F, Lian C, Yu J, Yang H, Lin S. Study on the adsorption performance and competitive mechanism for heavy metal contaminants removal using novel multi-pore activated carbons derived from recyclable long-root Eichhornia crassipes. Bioresour Technol. 2019;276:211–8.
doi: 10.1016/j.biortech.2019.01.007
pubmed: 30640014
Mariana M, HPS AK, Mistar EM, Yahya EB, Alfatah T, Danish M, Amayreh M. Recent advances in activated carbon modification techniques for enhanced heavy metal adsorption. J Water Proc Engin. 2021;43:102221.
doi: 10.1016/j.jwpe.2021.102221
Costa SF, Martins D, Agacka-Mołdoch M, Czubacka A, de Sousa Araújo S. Strategies to alleviate salinity stress in plants. Salinity Responses and Tolerance in Plants, Volume 1: Targeting Sensory, Transport and Signaling Mechanisms. 2018:307–337.
Valderrama A, Carvajal DE, Peñailillo P, Tapia J. Accumulation capacity of cadmium and copper and their effects on photosynthetic performance in Azolla filiculoides Lam. Under induced rhizofiltration. Gayana Bot. 2016;73(2):283–91.
doi: 10.4067/S0717-66432016000200283
Jagota N, Singh S, Kaur H, Kaur R, Sharma A. Oxidative stress in Pb toxicity in plants and its amelioration. InPb Toxicity Mitigation: Sustainable Nexus Approaches. Cham: Springer Nature Switzerland.2024:299–333.
Bindschedler LV, Minibayeva F, Gardner SL, Gerrish C, Davies DR, Bolwell GP. Early signalling events in the apoplastic oxidative burst in suspension cultured French bean cells involve cAMP and Ca
doi: 10.1046/j.1469-8137.2001.00170.x
pubmed: 33873377
Tirani MM, Haghjou MM. Reactive oxygen species (ROS), total antioxidant capacity (AOC) and malondialdehyde (MDA) make a triangle in evaluation of zinc stress extension. JAPS: J Anim Plant Sci. 2019;29(4).
Wagner GJ. Content and vacuole/extravacuole distribution of neutral sugars, free amino acids, and anthocyanin in protoplasts [of Hippeastrum petals and Tulipa petals and leaves].1979:88–93.
Tirani MM, Haghjou MM, Ismaili A. Hydroponic grown tobacco plants respond to zinc oxide nanoparticles and bulk exposures by morphological, physiological and anatomical adjustments. Func Plant Biol. 2019;46(4):360–75.
doi: 10.1071/FP18076
Bradford MM. A rapid and sensitive method for the quantitation of micro - gram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976;72(1–2):248–54.
doi: 10.1016/0003-2697(76)90527-3
pubmed: 942051
Lichtenthaler HK, Buschmann C. Chlorophylls and carotenoids: measurement and characterization by UV-VIS spectroscopy. Curr Protocols food Anal Chem. 2001;(1):F4–3.