Real-time PCR assay to detect the novel Clade Ib monkeypox virus, September 2023 to May 2024.

Monkeypox virus / genetics Real-Time Polymerase Chain Reaction / methods Mpox (monkeypox) / virology Sensitivity and Specificity Humans Animals Phylogeny DNA, Viral / genetics
Monkeypox virus Monkeypox virus Clade Ib Mpox virus Clade Ib Real-time PCR diagnostic test

Journal

Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin
ISSN: 1560-7917
Titre abrégé: Euro Surveill
Pays: Sweden
ID NLM: 100887452

Informations de publication

Date de publication:
Aug 2024
Historique:
medline: 9 8 2024
pubmed: 9 8 2024
entrez: 9 8 2024
Statut: ppublish

Résumé

Monkeypox virus (MPXV) is an emerging zoonotic pathogen with complex epidemiology necessitating rapid diagnosis and distinguishing between clades and subclades. The emerging Clade Ib lacks the genomic region used in the Clade I-specific assay from the Centers for Disease Control and Prevention. We report an MPXV real-time PCR to specifically detect Clade Ib. The assay demonstrated proficient sensitivity and specificity in 92 samples and can be included along other TaqMan-based assays to detect MPXV and distinguish between clades and subclades.

Identifiants

DOI: 10.2807/1560-7917.ES.2024.29.32.2400486 PMID: 39119722
pubmed: 39119722
doi: 10.2807/1560-7917.ES.2024.29.32.2400486
doi:

Substances chimiques

DNA, Viral 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Investigateurs

Marjan Boter (M)
Hayley Cassidy (H)
Léandre Mutimbwa Mambo (LM)
Gitta Overbeek (G)
Jean Pierre Musabyimana (JP)
Jules Ndoli (J)
Saria Otani (S)

Auteurs

Leonard Schuele (L)

These authors contributed equally to this work and share first authorship.
Department of Viroscience, Erasmus University Medical Center, Rotterdam, the Netherlands.

Leandre Murhula Masirika (LM)

These authors contributed equally to this work and share first authorship.
Congo Outbreaks, Research for Development, South Kivu, Bukavu, Democratic Republic of the Congo.
SaBio Instituto de Investigación en Recursos Cinegéticos IREC (Universidad de Castilla-La Mancha & CSIC), Ciudad Real, Spain.
Centre de Recherche en Sciences Naturelles de Lwiro, South Kivu, DS Bukavu, Democratic Republic of the Congo.

Jean Claude Udahemuka (JC)

These authors contributed equally to this work and share first authorship.
Genomics Research and Development Division, Stansile Research Organization, Kigali, Rwanda.
Department of Veterinary Medicine, University of Rwanda, Nyagatare, Rwanda.

Freddy Belesi Siangoli (FB)

Division Provinciale de la Santé, South Kivu, Bukavu, Democratic Republic of the Congo.

Justin Bengehya Mbiribindi (JB)

Division Provinciale de la Santé, South Kivu, Bukavu, Democratic Republic of the Congo.

Pacifique Ndishimye (P)

Research and Innovation Centre, African Institute for Mathematical Sciences (AIMS), Kigali, Rwanda.
Genomics Research and Development Division, Stansile Research Organization, Kigali, Rwanda.

Frank M Aarestrup (FM)

Research Group for Genomic Epidemiology, National Food Institute, Technical University of Denmark, Kgs. Lyngby, Denmark.

Marion Koopmans (M)

Department of Viroscience, Erasmus University Medical Center, Rotterdam, the Netherlands.

Bas B Oude Munnink (BB)

Department of Viroscience, Erasmus University Medical Center, Rotterdam, the Netherlands.

Richard Molenkamp (R)

Department of Viroscience, Erasmus University Medical Center, Rotterdam, the Netherlands.
The members of the network are listed under Collaborators.

Articles similaires

Comprehensive comparative analysis and development of molecular markers for Lasianthus species based on complete chloroplast genome sequences.

Yue Zhang, Meifang Song, Deying Tang et al.
1.00
Genome, Chloroplast Phylogeny Genetic Markers Base Composition High-Throughput Nucleotide Sequencing

[Redispensing of expensive oral anticancer medicines: a practical application].

Lisanne N van Merendonk, Kübra Akgöl, Bastiaan Nuijen
1.00
Humans Antineoplastic Agents Administration, Oral Drug Costs Counterfeit Drugs

Smoking Cessation and Incident Cardiovascular Disease.

Jun Hwan Cho, Seung Yong Shin, Hoseob Kim et al.
1.00
Humans Male Smoking Cessation Cardiovascular Diseases Female

Evaluation of Low-Value Services Across Major Medicare Advantage Insurers and Traditional Medicare.

Ciara Duggan, Adam L Beckman, Ishani Ganguli et al.
1.00
Humans United States Aged Cross-Sectional Studies Medicare Part C

Classifications MeSH

questionsmedicales.fr › Virus › Virus à ADN › Poxviridae › Chordopoxvirinae › Orthopoxvirus › Virus de la variole simienne › Real-time PCR assay to detect the novel Clade...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Techniques d'amplification d'acides nucléiques › Réaction de polymérisation en chaîne › Réaction de polymérisation en chaine en temps réel › Real-time PCR assay to detect the novel Clade...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Méthodes épidémiologiques › Statistiques comme sujet › Sensibilité et spécificité › Real-time PCR assay to detect the novel Clade...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Real-time PCR assay to detect the novel Clade...
questionsmedicales.fr › Eucaryotes › Animaux › Real-time PCR assay to detect the novel Clade...
questionsmedicales.fr › Sciences de l'information › Phylogenèse › Real-time PCR assay to detect the novel Clade...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ADN › ADN viral › Real-time PCR assay to detect the novel Clade...