Comparative transcriptomics reveals a highly polymorphic Xanthomonas HrpG virulence regulon.
Xanthomonas
HrpG
Regulon diversity
Transcriptome sequencing
Type III secretion system
Journal
BMC genomics
ISSN: 1471-2164
Titre abrégé: BMC Genomics
Pays: England
ID NLM: 100965258
Informations de publication
Date de publication:
09 Aug 2024
09 Aug 2024
Historique:
received:
21
05
2024
accepted:
02
08
2024
medline:
10
8
2024
pubmed:
10
8
2024
entrez:
9
8
2024
Statut:
epublish
Résumé
Bacteria of the genus Xanthomonas cause economically significant diseases in various crops. Their virulence is dependent on the translocation of type III effectors (T3Es) into plant cells by the type III secretion system (T3SS), a process regulated by the master response regulator HrpG. Although HrpG has been studied for over two decades, its regulon across diverse Xanthomonas species, particularly beyond type III secretion, remains understudied. In this study, we conducted transcriptome sequencing to explore the HrpG regulons of 17 Xanthomonas strains, encompassing six species and nine pathovars, each exhibiting distinct host and tissue specificities. We employed constitutive expression of plasmid-borne hrpG*, which encodes a constitutively active form of HrpG, to induce the regulon. Our findings reveal substantial inter- and intra-specific diversity in the HrpG* regulons across the strains. Besides 21 genes directly involved in the biosynthesis of the T3SS, the core HrpG* regulon is limited to only five additional genes encoding the transcriptional activator HrpX, the two T3E proteins XopR and XopL, a major facility superfamily (MFS) transporter, and the phosphatase PhoC. Interestingly, genes involved in chemotaxis and genes encoding enzymes with carbohydrate-active and proteolytic activities are variably regulated by HrpG*. The diversity in the HrpG* regulon suggests that HrpG-dependent virulence in Xanthomonas might be achieved through several distinct strain-specific strategies, potentially reflecting adaptation to diverse ecological niches. These findings enhance our understanding of the complex role of HrpG in regulating various virulence and adaptive pathways, extending beyond T3Es and the T3SS.
Sections du résumé
BACKGROUND
BACKGROUND
Bacteria of the genus Xanthomonas cause economically significant diseases in various crops. Their virulence is dependent on the translocation of type III effectors (T3Es) into plant cells by the type III secretion system (T3SS), a process regulated by the master response regulator HrpG. Although HrpG has been studied for over two decades, its regulon across diverse Xanthomonas species, particularly beyond type III secretion, remains understudied.
RESULTS
RESULTS
In this study, we conducted transcriptome sequencing to explore the HrpG regulons of 17 Xanthomonas strains, encompassing six species and nine pathovars, each exhibiting distinct host and tissue specificities. We employed constitutive expression of plasmid-borne hrpG*, which encodes a constitutively active form of HrpG, to induce the regulon. Our findings reveal substantial inter- and intra-specific diversity in the HrpG* regulons across the strains. Besides 21 genes directly involved in the biosynthesis of the T3SS, the core HrpG* regulon is limited to only five additional genes encoding the transcriptional activator HrpX, the two T3E proteins XopR and XopL, a major facility superfamily (MFS) transporter, and the phosphatase PhoC. Interestingly, genes involved in chemotaxis and genes encoding enzymes with carbohydrate-active and proteolytic activities are variably regulated by HrpG*.
CONCLUSIONS
CONCLUSIONS
The diversity in the HrpG* regulon suggests that HrpG-dependent virulence in Xanthomonas might be achieved through several distinct strain-specific strategies, potentially reflecting adaptation to diverse ecological niches. These findings enhance our understanding of the complex role of HrpG in regulating various virulence and adaptive pathways, extending beyond T3Es and the T3SS.
Identifiants
pubmed: 39123115
doi: 10.1186/s12864-024-10684-6
pii: 10.1186/s12864-024-10684-6
doi:
Substances chimiques
Bacterial Proteins
0
Type III Secretion Systems
0
Transcription Factors
0
Types de publication
Journal Article
Comparative Study
Langues
eng
Sous-ensembles de citation
IM
Pagination
777Subventions
Organisme : Agence Nationale de la Recherche
ID : ANR-19-CE20-JCJC-0014-01
Organisme : Agence Nationale de la Recherche
ID : ANR-2010-GENM-013-02
Organisme : Agence Nationale de la Recherche
ID : ANR-2010-GENM-013-02
Organisme : Agence Nationale de la Recherche
ID : ANR-2010-GENM-013-02
Organisme : Agence Nationale de la Recherche
ID : ANR-2010-GENM-013-02
Organisme : Agence Nationale de la Recherche
ID : ANR-2010-GENM-013-02
Organisme : Agence Nationale de la Recherche
ID : ANR-2010-GENM-013-02
Organisme : Agence Nationale de la Recherche
ID : ANR-2010-GENM-013-02
Organisme : Agence Nationale de la Recherche
ID : ANR-2010-GENM-013-02
Organisme : Agence Nationale de la Recherche
ID : ANR-2010-GENM-013-02
Organisme : Agence Nationale de la Recherche
ID : ANR-2010-GENM-013-02
Organisme : Agence Nationale de la Recherche
ID : ANR-2010-GENM-013-02
Organisme : Agence Nationale de la Recherche
ID : ANR-2010-GENM-013-02
Organisme : Agence Nationale de la Recherche
ID : ANR-2010-GENM-013-02
Organisme : Agence Nationale de la Recherche
ID : ANR-2010-GENM-013-02
Organisme : Agence Nationale de la Recherche
ID : ANR-19-CE20-JCJC-0014-01
Organisme : Laboratoire d'Excellence TULIP
ID : ANR-10-LABX-41
Organisme : Laboratoire d'Excellence TULIP
ID : ANR-10-LABX-41
Organisme : Laboratoire d'Excellence TULIP
ID : ANR-10-LABX-41
Organisme : Laboratoire d'Excellence TULIP
ID : ANR-10-LABX-41
Organisme : Laboratoire d'Excellence TULIP
ID : ANR-10-LABX-41
Organisme : Laboratoire d'Excellence TULIP
ID : ANR-10-LABX-41
Organisme : Laboratoire d'Excellence TULIP
ID : ANR-10-LABX-41
Organisme : Laboratoire d'Excellence TULIP
ID : ANR-10-LABX-41
Informations de copyright
© 2024. The Author(s).
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