Signal-On Detection of Caspase-3 with Methylene Blue-Loaded Metal-Organic Frameworks as Signal Reporters.

Metal-Organic Frameworks / chemistry Methylene Blue / chemistry Humans Caspase 3 / metabolism HeLa Cells Biosensing Techniques / methods Electrochemical Techniques / methods Apoptosis Streptavidin / chemistry Biotinylation Electrodes Limit of Detection Zirconium / chemistry Phthalic Acids
biotinylation caspase-3 cell apoptosis electrochemical biosensor metal–organic frameworks

Journal

Molecules (Basel, Switzerland)
ISSN: 1420-3049
Titre abrégé: Molecules
Pays: Switzerland
ID NLM: 100964009

Informations de publication

Date de publication:
05 Aug 2024
Historique:
received: 21 06 2024
revised: 01 08 2024
accepted: 02 08 2024
medline: 10 8 2024
pubmed: 10 8 2024
entrez: 10 8 2024
Statut: epublish

Résumé

In this work, we report on an electrochemical method for the signal-on detection of caspase-3 and the evaluation of apoptosis based on the biotinylation reaction and the signal amplification of methylene blue (MB)-loaded metal-organic frameworks (MOFs). Zr-based UiO-66-NH

Identifiants

DOI: 10.3390/molecules29153700 PMID: 39125104
pubmed: 39125104
pii: molecules29153700
doi: 10.3390/molecules29153700
pii:
doi:

Substances chimiques

Metal-Organic Frameworks 0
Methylene Blue T42P99266K
Caspase 3 EC 3.4.22.-
Streptavidin 9013-20-1
UiO-66 0
Zirconium C6V6S92N3C
Phthalic Acids 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : Program for Innovative Research Team of Science and Technology in Anyang Normal University
ID : 2023AYSYKYCXTD03

Auteurs

Yaliang Huang (Y)

School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China.
College of Chemistry and Chemical Engineering, Anyang Normal University, Anyang 455000, China.
College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.

Jiaqiang Wang (J)

College of Chemistry and Chemical Engineering, Anyang Normal University, Anyang 455000, China.

Yirui Xu (Y)

School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China.

Jiwen Zhang (J)

College of Chemistry and Chemical Engineering, Anyang Normal University, Anyang 455000, China.

Ning Xia (N)

College of Chemistry and Chemical Engineering, Anyang Normal University, Anyang 455000, China.
ORCID: 0000-0003-0366-7524

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Classifications MeSH

questionsmedicales.fr › Structures macromoléculaires › Réseaux organométalliques › Signal-On Detection of Caspase-3 with...
questionsmedicales.fr › Composés hétérocycliques › Composés hétérocycliques à cycles fusionnés › Composés hétérocycliques 3 noyaux › Phénothiazines › Bleu de méthylène › Signal-On Detection of Caspase-3 with...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Signal-On Detection of Caspase-3 with...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines et peptides de signalisation intracellulaire › Protéines régulatrices de l'apoptose › Caspases › Caspases effectrices › Caspase-3 › Signal-On Detection of Caspase-3 with...
questionsmedicales.fr › Cellules › Cellules épithéliales › Cellules HeLa › Signal-On Detection of Caspase-3 with...
questionsmedicales.fr › Techniques d'investigation › Techniques de sonde moléculaire › Techniques de biocapteur › Signal-On Detection of Caspase-3 with...
questionsmedicales.fr › Techniques d'investigation › Techniques électrochimiques › Signal-On Detection of Caspase-3 with...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Mort cellulaire › Mort cellulaire régulée › Apoptose › Signal-On Detection of Caspase-3 with...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines bactériennes › Streptavidine › Signal-On Detection of Caspase-3 with...
questionsmedicales.fr › Métabolisme › Biotinylation › Signal-On Detection of Caspase-3 with...
questionsmedicales.fr › Équipement et fournitures › Équipement et fournitures électriques › Électrodes › Signal-On Detection of Caspase-3 with...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Méthodes épidémiologiques › Statistiques comme sujet › Sensibilité et spécificité › Limite de détection › Signal-On Detection of Caspase-3 with...
questionsmedicales.fr › Produits chimiques inorganiques › Métaux › Métaux lourds › Zirconium › Signal-On Detection of Caspase-3 with...
questionsmedicales.fr › Composés chimiques organiques › Acides carboxyliques › Acides carbocycliques › Acides phtaliques › Signal-On Detection of Caspase-3 with...