Effects of HTLV-1 on leukocyte trafficking and migration in ACs compared to healthy individuals.
Humans
Human T-lymphotropic virus 1
/ genetics
Male
Female
Adult
Case-Control Studies
Middle Aged
Cell Movement
HTLV-I Infections
/ immunology
Gene Products, tax
/ genetics
RNA, Messenger
/ genetics
Receptors, CXCR4
/ genetics
rho-Associated Kinases
/ genetics
Leukocytes
/ metabolism
Proto-Oncogene Proteins c-vav
/ genetics
Lymphocyte Function-Associated Antigen-1
/ metabolism
Retroviridae Proteins
Basic-Leucine Zipper Transcription Factors
ACs
ATLL
Cell migration
HAM/TSP
HTLV-1
MRNA expression
Journal
BMC research notes
ISSN: 1756-0500
Titre abrégé: BMC Res Notes
Pays: England
ID NLM: 101462768
Informations de publication
Date de publication:
10 Aug 2024
10 Aug 2024
Historique:
received:
30
01
2024
accepted:
05
08
2024
medline:
11
8
2024
pubmed:
11
8
2024
entrez:
10
8
2024
Statut:
epublish
Résumé
Human T-lymphotropic virus type 1 (HTLV-1) is a RNA virus belonging to Retroviridae family and is associated with the development of various diseases, including adult T-cell leukemia/lymphoma (ATLL) and HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP). Aside from HAM/TSP, HTLV-1 has been implicated in the development of several disorders that mimic auto-inflammation. T-cell migration is important topic in the context of HTLV-1 associated diseases progression. The primary objective of this case-control study was to assess the relationship between increased mRNA expression in virus migration following HTLV-1 infection. PBMCs from 20 asymptomatic patients and 20 healthy subjects were analyzed using real-time PCR to measure mRNA expression of LFA1, MLCK, RAC1, RAPL, ROCK1, VAV1 and CXCR4. Also, mRNA expression of Tax and HBZ were evaluated. Mean expression of Tax and HBZ in ACs (asymptomatic carriers) was 0.7218 and 0.6517 respectively. The results revealed a noteworthy upregulation of these genes involved in T-cell migration among ACs patients in comparison to healthy individuals. Considering the pivotal role of gene expression alterations associated with the progression into two major diseases (ATLL or HAM/TSP), analyzing the expression of these genes in the ACs group can offer probable potential diagnostic markers and aid in monitoring the condition of ACs.
Identifiants
pubmed: 39127702
doi: 10.1186/s13104-024-06887-5
pii: 10.1186/s13104-024-06887-5
doi:
Substances chimiques
Gene Products, tax
0
RNA, Messenger
0
HBZ protein, human T-cell leukemia virus type I
0
Receptors, CXCR4
0
rho-Associated Kinases
EC 2.7.11.1
VAV1 protein, human
0
CXCR4 protein, human
0
Proto-Oncogene Proteins c-vav
0
ROCK1 protein, human
EC 2.7.11.1
tax protein, Human T-lymphotrophic virus 1
0
Lymphocyte Function-Associated Antigen-1
0
Retroviridae Proteins
0
Basic-Leucine Zipper Transcription Factors
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
222Informations de copyright
© 2024. The Author(s).
Références
Grassmann R, Aboud M, Jeang K-T. Molecular mechanisms of cellular transformation by HTLV-1 Tax. Oncogene. 2005;24(39):5976–85.
doi: 10.1038/sj.onc.1208978
pubmed: 16155604
Cook LB, Elemans M, Rowan AG, Asquith B. HTLV-1: persistence and pathogenesis. Virology. 2013;435(1):131–40.
doi: 10.1016/j.virol.2012.09.028
pubmed: 23217623
Thomsen AR, Nansen A, Madsen AN, Bartholdy C, Christensen JP. Regulation of T cell migration during viral infection: role of adhesion molecules and chemokines. Immunol Lett. 2003;85(2):119–27.
doi: 10.1016/S0165-2478(02)00236-5
pubmed: 12527217
Trepat X, Chen Z, Jacobson K. Cell migration. Compr Physiol. 2012;2(4):2369.
doi: 10.1002/cphy.c110012
pubmed: 23720251
pmcid: 4457291
Hieshima K, Nagakubo D, Nakayama T, Shirakawa A-K, Jin Z, Yoshie O. Tax-inducible production of CC chemokine ligand 22 by human T cell leukemia virus type 1 (HTLV-1)-infected T cells promotes preferential transmission of HTLV-1 to CCR4-expressing CD4+ T cells. J Immunol. 2008;180(2):931–9.
doi: 10.4049/jimmunol.180.2.931
pubmed: 18178833
Mothes W, Sherer NM, Jin J, Zhong P. Virus cell-to-cell transmission. J Virol. 2010;84(17):8360–8.
doi: 10.1128/JVI.00443-10
pubmed: 20375157
pmcid: 2918988
Poon B. Virus–host interactions of human retroviruses. Los Angeles: University of California; 1997.
Kedrin D, van Rheenen J, Hernandez L, Condeelis J, Segall JE. Cell motility and cytoskeletal regulation in invasion and metastasis. J Mammary Gland Biol Neoplasia. 2007;12:143–52.
doi: 10.1007/s10911-007-9046-4
pubmed: 17557195
Man S, Ubogu EE, Ransohoff RM. Inflammatory cell migration into the central nervous system: a few new twists on an old tale. Brain Pathol. 2007;17(2):243–50.
doi: 10.1111/j.1750-3639.2007.00067.x
pubmed: 17388955
pmcid: 8095646
Engelhardt B, Ransohoff RM. Capture, crawl, cross: the T cell code to breach the blood–brain barriers. Trends Immunol. 2012;33(12):579–89.
doi: 10.1016/j.it.2012.07.004
pubmed: 22926201
Shayeghpour A, Forghani-Ramandi M-M, Solouki S, Hosseini A, Hosseini P, Khodayar S, et al. Identification of novel miRNAs potentially involved in the pathogenesis of adult T-cell leukemia/lymphoma using WGCNA followed by RT-qPCR test of hub genes. Infect Agents Cancer. 2023;18(1):1–11.
doi: 10.1186/s13027-023-00492-0
Reina M, Espel E. Role of LFA-1 and ICAM-1 in cancer. Cancers. 2017;9(11):153.
doi: 10.3390/cancers9110153
pubmed: 29099772
pmcid: 5704171
Walling BL, Kim M. LFA-1 in T cell migration and differentiation. Front Immunol. 2018;9:952.
doi: 10.3389/fimmu.2018.00952
pubmed: 29774029
pmcid: 5943560
Shen W, Zhang X, Du R, Fan Y, Luo D, Bao Y, et al. ICAM3 mediates tumor metastasis via a LFA-1-ICAM3-ERM dependent manner. Biochim Biophys Acta (BBA) Mol Basis Dis. 2018;1864(8):2566–78.
doi: 10.1016/j.bbadis.2018.05.002
Goodman SL, Picard M. Integrins as therapeutic targets. Trends Pharmacol Sci. 2012;33(7):405–12.
doi: 10.1016/j.tips.2012.04.002
pubmed: 22633092
Goeckeler ZM, Wysolmerski RB. Myosin light chain kinase-regulated endothelial cell contraction: the relationship between isometric tension, actin polymerization, and myosin phosphorylation. J Cell Biol. 1995;130(3):613–27.
doi: 10.1083/jcb.130.3.613
pubmed: 7622562
Kohama K, Ye L-H, Hayakawa K, Okagaki T. Myosin light chain kinase: an actin-binding protein that regulates an ATP-dependent interaction with myosin. Trends Pharmacol Sci. 1996;17(8):284–7.
doi: 10.1016/0165-6147(96)10033-X
pubmed: 8810874
Bhat AA, Uppada S, Achkar IW, Hashem S, Yadav SK, Shanmugakonar M, et al. Tight junction proteins and signaling pathways in cancer and inflammation: a functional crosstalk. Front Physiol. 2019;9:1942.
doi: 10.3389/fphys.2018.01942
pubmed: 30728783
pmcid: 6351700
Lin C-Y, Zu C-H, Yang C-C, Tsai P-J, Shyu J-F, Chen C-P, et al. IL-1β-induced mesenchymal stem cell migration involves MLCK activation via PKC signaling. Cell Transplant. 2015;24(10):2011–28.
doi: 10.3727/096368914X685258
pubmed: 25333338
Kim D, Helfman DM. Loss of MLCK leads to disruption of cell–cell adhesion and invasive behavior of breast epithelial cells via increased expression of EGFR and ERK/JNK signaling. Oncogene. 2016;35(34):4495–508.
doi: 10.1038/onc.2015.508
pubmed: 26876209
Nejmeddine M, Barnard AL, Tanaka Y, Taylor GP, Bangham CR. Human T-lymphotropic virus, type 1, tax protein triggers microtubule reorientation in the virological synapse. J Biol Chem. 2005;280(33):29653–60.
doi: 10.1074/jbc.M502639200
pubmed: 15975923
Zhang Y-L, Wang R-C, Cheng K, Ring BZ, Su L. Roles of Rap1 signaling in tumor cell migration and invasion. Cancer Biol Med. 2017;14(1):90.
doi: 10.20892/j.issn.2095-3941.2016.0086
pubmed: 28443208
pmcid: 5365179
Laufer JM, Legler DF. Beyond migration—chemokines in lymphocyte priming, differentiation, and modulating effector functions. J Leukoc Biol. 2018;104(2):301–12.
doi: 10.1002/JLB.2MR1217-494R
pubmed: 29668063
Kinashi T, Katagiri K. Regulation of lymphocyte adhesion and migration by the small GTPase Rap1 and its effector molecule. RAPL Immunol Lett. 2004;93(1):1–5.
doi: 10.1016/j.imlet.2004.02.008
pubmed: 15134891
Liu BP, Burridge K. Vav2 activates Rac1, Cdc42, and RhoA downstream from growth factor receptors but not β1 integrins. Mol Cell Biol. 2000;20(19):7160–9.
doi: 10.1128/MCB.20.19.7160-7169.2000
pubmed: 10982832
pmcid: 86269
Valderrama F, Thevapala S, Ridley AJ. Radixin regulates cell migration and cell–cell adhesion through Rac1. J Cell Sci. 2012;125(14):3310–9.
pubmed: 22467863
Reynolds LF, Smyth LA, Norton T, Freshney N, Downward J, Kioussis D, Tybulewicz VL. Vav1 transduces T cell receptor signals to the activation of phospholipase C-γ1 via phosphoinositide 3-kinase-dependent and-independent pathways. J Exp Med. 2002;195(9):1103–14.
doi: 10.1084/jem.20011663
pubmed: 11994416
pmcid: 2193701
Shah VB, Ozment-Skelton TR, Williams DL, Keshvara L. Vav1 and PI3K are required for phagocytosis of β-glucan and subsequent superoxide generation by microglia. Mol Immunol. 2009;46(8–9):1845–53.
doi: 10.1016/j.molimm.2009.01.014
pubmed: 19232731
Montalvo J, Spencer C, Hackathorn A, Masterjohn K, Perkins A, Doty C, et al. ROCK1 & 2 perform overlapping and unique roles in angiogenesis and angiosarcoma tumor progression. Curr Mol Med. 2013;13(1):205–19.
doi: 10.2174/156652413804486296
pubmed: 22934846
pmcid: 3580831
Gagliardi PA, di Blasio L, Primo L. PDK1: a signaling hub for cell migration and tumor invasion. Biochim Biophys Acta (BBA) Rev Cancer. 2015;1856(2):178–88.
doi: 10.1016/j.bbcan.2015.07.003
Hu C, Zhou H, Liu Y, Huang J, Liu W, Zhang Q, et al. ROCK1 promotes migration and invasion of non-small-cell lung cancer cells through the PTEN/PI3K/FAK pathway. Int J Oncol. 2019;55(4):833–44.
pubmed: 31485605
pmcid: 6741846
Zhang R, Li G, Zhang Q, Tang Q, Huang J, Hu C, et al. Hirsutine induces mPTP-dependent apoptosis through ROCK1/PTEN/PI3K/GSK3β pathway in human lung cancer cells. Cell Death Dis. 2018;9(6):598.
doi: 10.1038/s41419-018-0641-7
pubmed: 29789524
pmcid: 5964100
Li G-B, Cheng Q, Liu L, Zhou T, Shan C-Y, Hu X-Y, et al. Mitochondrial translocation of cofilin is required for allyl isothiocyanate-mediated cell death via ROCK1/PTEN/PI3K signaling pathway. Cell Commun Signaling. 2013;11(1):1–13.
doi: 10.1186/1478-811X-11-50
Zhao Y, Lv M, Lin H, Hong Y, Yang F, Sun Y, et al. ROCK1 induces ERK nuclear translocation in PDGF-BB-stimulated migration of rat vascular smooth muscle cells. IUBMB Life. 2012;64(2):194–202.
doi: 10.1002/iub.598
pubmed: 22215561
Noma K, Rikitake Y, Oyama N, Yan G, Alcaide P, Liu P-Y, et al. ROCK1 mediates leukocyte recruitment and neointima formation following vascular injury. J Clin Investig. 2008;118(5):1632–44.
doi: 10.1172/JCI29226
pubmed: 18414683
pmcid: 2293333
Moriuchi M, Moriuchi H, Fauci AS. HTLV-I Tax activation of the cxcr4 promoter by association with nuclear respiratory factor 1. JAIDS J Acquir Immune Defic Syndr. 1999;20(4):A77.
Moriuchi M, Moriuchi H, Fauci AS. Short communication HTLV type I Tax activation of the CXCR4 promoter by association with nuclear respiratory factor 1. AIDS Res Hum Retrovir. 1999;15(9):821–7.
doi: 10.1089/088922299310728
pubmed: 10381170