Cellular transitions during cranial suture establishment in zebrafish.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
13 Aug 2024
13 Aug 2024
Historique:
received:
17
03
2023
accepted:
19
07
2024
medline:
14
8
2024
pubmed:
14
8
2024
entrez:
13
8
2024
Statut:
epublish
Résumé
Cranial sutures separate neighboring skull bones and are sites of bone growth. A key question is how osteogenic activity is controlled to promote bone growth while preventing aberrant bone fusions during skull expansion. Using single-cell transcriptomics, lineage tracing, and mutant analysis in zebrafish, we uncover key developmental transitions regulating bone formation at sutures during skull expansion. In particular, we identify a subpopulation of mesenchyme cells in the mid-suture region that upregulate a suite of genes including BMP antagonists (e.g. grem1a) and pro-angiogenic factors. Lineage tracing with grem1a:nlsEOS reveals that this mid-suture subpopulation is largely non-osteogenic. Moreover, combinatorial mutation of BMP antagonists enriched in this mid-suture subpopulation results in increased BMP signaling in the suture, misregulated bone formation, and abnormal suture morphology. These data reveal establishment of a non-osteogenic mesenchyme population in the mid-suture region that restricts bone formation through local BMP antagonism, thus ensuring proper suture morphology.
Identifiants
pubmed: 39138165
doi: 10.1038/s41467-024-50780-5
pii: 10.1038/s41467-024-50780-5
doi:
Substances chimiques
Zebrafish Proteins
0
Bone Morphogenetic Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
6948Subventions
Organisme : U.S. Department of Health & Human Services | National Institutes of Health (NIH)
ID : 5R01DE026339
Organisme : U.S. Department of Health & Human Services | National Institutes of Health (NIH)
ID : 5R01DE026339
Organisme : U.S. Department of Health & Human Services | National Institutes of Health (NIH)
ID : 5R01DE026339
Informations de copyright
© 2024. This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply.
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