Sulfur starvation-induced autophagy in Saccharomyces cerevisiae involves SAM-dependent signaling and transcription activator Met4.
Saccharomyces cerevisiae
/ metabolism
Autophagy
/ genetics
Saccharomyces cerevisiae Proteins
/ metabolism
Sulfur
/ metabolism
Signal Transduction
S-Adenosylmethionine
/ metabolism
Gene Expression Regulation, Fungal
Autophagy-Related Proteins
/ metabolism
Methionine
/ metabolism
Transcription Factors
/ metabolism
Protein Kinases
Basic-Leucine Zipper Transcription Factors
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
13 Aug 2024
13 Aug 2024
Historique:
received:
03
05
2022
accepted:
01
08
2024
medline:
14
8
2024
pubmed:
14
8
2024
entrez:
13
8
2024
Statut:
epublish
Résumé
Autophagy is a key lysosomal degradative mechanism allowing a prosurvival response to stresses, especially nutrient starvation. Here we investigate the mechanism of autophagy induction in response to sulfur starvation in Saccharomyces cerevisiae. We found that sulfur deprivation leads to rapid and widespread transcriptional induction of autophagy-related (ATG) genes in ways not seen under nitrogen starvation. This distinctive response depends mainly on the transcription activator of sulfur metabolism Met4. Consistently, Met4 is essential for autophagy under sulfur starvation. Depletion of either cysteine, methionine or SAM induces autophagy flux. However, only SAM depletion can trigger strong transcriptional induction of ATG genes and a fully functional autophagic response. Furthermore, combined inactivation of Met4 and Atg1 causes a dramatic decrease in cell survival under sulfur starvation, highlighting the interplay between sulfur metabolism and autophagy to maintain cell viability. Thus, we describe a pathway of sulfur starvation-induced autophagy depending on Met4 and involving SAM as signaling sulfur metabolite.
Identifiants
pubmed: 39138175
doi: 10.1038/s41467-024-51309-6
pii: 10.1038/s41467-024-51309-6
doi:
Substances chimiques
Saccharomyces cerevisiae Proteins
0
Sulfur
70FD1KFU70
MET4 protein, S cerevisiae
0
S-Adenosylmethionine
7LP2MPO46S
Autophagy-Related Proteins
0
Methionine
AE28F7PNPL
ATG1 protein, S cerevisiae
EC 2.7.1.-
Transcription Factors
0
Protein Kinases
EC 2.7.-
Basic-Leucine Zipper Transcription Factors
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
6927Informations de copyright
© 2024. The Author(s).
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