Lymphangiogenesis in the liver of biliary atresia.
Biliary atresia
Lymphangiogenesis
Lymphatic vessels
Journal
BMC gastroenterology
ISSN: 1471-230X
Titre abrégé: BMC Gastroenterol
Pays: England
ID NLM: 100968547
Informations de publication
Date de publication:
15 Aug 2024
15 Aug 2024
Historique:
received:
12
04
2024
accepted:
13
08
2024
medline:
15
8
2024
pubmed:
15
8
2024
entrez:
14
8
2024
Statut:
epublish
Résumé
Lymphatic vessels (LVs) play a crucial role in immune reactions by serving as the principal conduits for immune cells. However, to date, no study has analyzed the morphological changes in the LVs of patients with biliary atresia (BA). In this study, we aimed to determine the morphological changes in the LVs irrigating the liver in patients with BA, elucidate their correlations with the morphology of the portal vein (PV) branches, and discuss their etiopathogenetic significance. Morphometric analyses of liver biopsy specimens from patients treated between 1986 and 2016 were performed. The parameters measured were as follows: the whole liver area of the specimen, fibrotic area, number of LVs, LVs without patent lumen (designated as Ly0) and PV branches, and diameters of the LVs with patent lumen and the PVs. The numbers of LVs, Ly0, and PV branches per unit area of the whole liver specimen were significantly higher in patients with BA than in control participants with liver disease and those with normal livers. However, no correlation was observed between the fibrotic area and the average diameter of LVs or PVs, and between the fibrotic area and the number of LVs or PV branches. Furthermore, no correlation was observed between the total number of LVs and the number of PV branches. The present study showed a significant increase in the number of total LVs and Ly0, characterized by a high Ly0 to total LVs ratio, suggesting that lymphangiogenesis occurs in the liver of patients with BA.
Sections du résumé
BACKGROUND
BACKGROUND
Lymphatic vessels (LVs) play a crucial role in immune reactions by serving as the principal conduits for immune cells. However, to date, no study has analyzed the morphological changes in the LVs of patients with biliary atresia (BA). In this study, we aimed to determine the morphological changes in the LVs irrigating the liver in patients with BA, elucidate their correlations with the morphology of the portal vein (PV) branches, and discuss their etiopathogenetic significance.
METHODS
METHODS
Morphometric analyses of liver biopsy specimens from patients treated between 1986 and 2016 were performed. The parameters measured were as follows: the whole liver area of the specimen, fibrotic area, number of LVs, LVs without patent lumen (designated as Ly0) and PV branches, and diameters of the LVs with patent lumen and the PVs.
RESULTS
RESULTS
The numbers of LVs, Ly0, and PV branches per unit area of the whole liver specimen were significantly higher in patients with BA than in control participants with liver disease and those with normal livers. However, no correlation was observed between the fibrotic area and the average diameter of LVs or PVs, and between the fibrotic area and the number of LVs or PV branches. Furthermore, no correlation was observed between the total number of LVs and the number of PV branches.
CONCLUSIONS
CONCLUSIONS
The present study showed a significant increase in the number of total LVs and Ly0, characterized by a high Ly0 to total LVs ratio, suggesting that lymphangiogenesis occurs in the liver of patients with BA.
Identifiants
pubmed: 39143576
doi: 10.1186/s12876-024-03370-0
pii: 10.1186/s12876-024-03370-0
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
266Informations de copyright
© 2024. The Author(s).
Références
Nio M, Ohi R. Biliary atresia. Semin Pediatr Surg. 2000;9:177–86.
doi: 10.1053/spsu.2000.18846
pubmed: 11112835
Russo P, Magee JC, Anders RA, Bove KE, Chung C, Cummings OW, et al. Key histopathologic features of liver biopsies that distinguish biliary atresia from other causes of infantile cholestasis and their correlation with outcome: a multicenter study. Am J Surg Pathol. 2016;40:1601–15.
doi: 10.1097/PAS.0000000000000755
pubmed: 27776008
pmcid: 5123664
Nio M. Japanese biliary atresia registry. Pediatr Surg Int. 2017;33:1319–25.
doi: 10.1007/s00383-017-4160-x
pubmed: 29039049
Petersen C, Davenport M. Aetiology of biliary atresia: what is actually known? Orphanet J Rare Dis. 2013;8:128.
doi: 10.1186/1750-1172-8-128
pubmed: 23987231
pmcid: 3766137
Feldman AG, Mack CL. Biliary atresia: clinical lessons learned. J Pediatr Gastroenterol Nutr. 2015;61:167–75.
doi: 10.1097/MPG.0000000000000755
pubmed: 25658057
Muraji T. Maternal microchimerism in biliary atresia: are maternal cells effector cells, targets, or just bystanders? Chimerism. 2014;5:1–5.
doi: 10.4161/chim.28576
pubmed: 24670921
pmcid: 3988115
Vollmar B, Wolf B, Siegmund S, Katsen AD, Menger MD. Lymph vessel expansion and function in the development of hepatic fibrosis and cirrhosis. Am J Pathol. 1997;151:169–75.
pubmed: 9212743
pmcid: 1857941
Tanaka M, Iwakiri Y. The hepatic lymphatic vascular system: structure, function, markers, and lymphangiogenesis. Cell Mol Gastroenterol Hepatol. 2016;2:733–49.
doi: 10.1016/j.jcmgh.2016.09.002
pubmed: 28105461
pmcid: 5240041
Masuya R, Muraji T, Ohtani H, Mukai M, Onishi S, Harumatsu T, et al. Morphometric demonstration of portal vein stenosis and hepatic arterial medial hypertrophy in patients with biliary atresia. Pediatr Surg Int. 2019;35:529–37.
doi: 10.1007/s00383-019-04459-4
pubmed: 30762106
Srinivasan RS, Dillard ME, Lagutin OV, Lin FJ, Tsai S, Tsai MJ, et al. Lineage tracing demonstrates the venous origin of the mammalian lymphatic vasculature. Genes Dev. 2007;21:2422–32.
doi: 10.1101/gad.1588407
pubmed: 17908929
pmcid: 1993873
Ohuchi N, Ohi R, Takahashi T, Kasai M. Postoperative changes of intrahepatic portal veins in biliary atresia–a 3-D reconstruction study. J Pediatr Surg. 1986;21:10–4.
doi: 10.1016/S0022-3468(86)80641-8
pubmed: 3944749
Kasai M, Okamoto A, Ohi R, Yabe K, Matsumura Y. Changes of portal vein pressure and intrahepatic blood vessels after surgery for biliary atresia. J Pediatr Surg. 1981;16(2):152–9.
doi: 10.1016/S0022-3468(81)80342-9
pubmed: 7241316
Harumatsu T, Muraji T, Masuya R, Ohtani H, Nagai T, Yano K, et al. Microvascular proliferation of the portal vein branches in the liver of biliary atresia patients at Kasai operation is associated with a better long-term clinical outcome. Pediatr Surg Int. 2019;35:1437–41.
doi: 10.1007/s00383-019-04579-x
pubmed: 31542827
Muraji T, Masuya R, Harumatsu T, Kawano T, Muto M, Ieiri S. New insights in understanding biliary atresia from the perspectives on maternal microchimerism. Front Pediatr. 2022;10:1007987.
doi: 10.3389/fped.2022.1007987
pubmed: 36210938
pmcid: 9539747
Oikawa H, Masuda T, Sato S, Yashima A, Suzuki K, Sato S, et al. Changes in lymph vessels and portal veins in the portal tract of patients with idiopathic portal hypertension: a morphometric study. Hepatology. 1998;27:1607–10.
doi: 10.1002/hep.510270621
pubmed: 9620334
Mertlitz S, Shi Y, Kalupa M, Grotzinger C, Mengwasser J, Riesner K, et al. Lymphangiogenesis is a feature of acute GVHD, and VEGFR-3 inhibition protects against experimental GVHD. Blood. 2017;129:1865–75.
doi: 10.1182/blood-2016-08-734210
pubmed: 28096093
Ishii E, Shimizu A, Kuwahara N, Arai T, Kataoka M, Wakamatsu K, et al. Lymphangiogenesis associated with acute cellular rejection in rat liver transplantation. Transplant Proc. 2010;42:4282–5.
doi: 10.1016/j.transproceed.2010.09.081
pubmed: 21168683
Chung C, Iwakiri Y. The lymphatic vascular system in liver diseases: its role in ascites formation. Clin Mol Hepatol. 2013;19:99–104.
doi: 10.3350/cmh.2013.19.2.99
pubmed: 23837133
pmcid: 3701854
Tanaka M, Iwakiri Y. Lymphatics in the liver. Curr Opin Immunol. 2018;53:137–42.
doi: 10.1016/j.coi.2018.04.028
pubmed: 29772409
pmcid: 6986420
Suda K, Muraji T, Ohtani H, Aiyoshi T, Sasaki T, Toma M, et al. Histological significance of hepatitis-like findings in biliary atresia: an analysis of 34 Japanese cases. Pediatr Int. 2019;61:364–8.
doi: 10.1111/ped.13816
pubmed: 30811786
Duarte RF, Delgado J, Shaw BE, Wrench DJ, Ethell M, Patch D, et al. Histologic features of the liver biopsy predict the clinical outcome for patients with graft-versus-host disease of the liver. Biol Blood Marrow Transplant. 2005;11:805–13.
doi: 10.1016/j.bbmt.2005.06.008
pubmed: 16182181
Salomao M, Dorritie K, Mapara MY, Sepulveda A. Histopathology of graft-vs-host disease of gastrointestinal tract and liver: an update. Am J Clin Pathol. 2016;145:591–603.
doi: 10.1093/ajcp/aqw050
pubmed: 27247365
Shulman HM, Sharma P, Amos D, Fenster LF, McDonald GB. A coded histologic study of hepatic graft-versus-host disease after human bone marrow transplantation. Hepatology. 1988;8:463–70.
doi: 10.1002/hep.1840080305
pubmed: 3131226