Vitamin B
biotransformation
compound‐specific isotope analysis
dual‐isotope analysis
enzyme kinetics
organohalide respiration
reductive dechlorination
Journal
MicrobiologyOpen
ISSN: 2045-8827
Titre abrégé: Microbiologyopen
Pays: England
ID NLM: 101588314
Informations de publication
Date de publication:
Aug 2024
Aug 2024
Historique:
revised:
03
07
2024
received:
03
05
2024
accepted:
01
08
2024
medline:
27
8
2024
pubmed:
27
8
2024
entrez:
27
8
2024
Statut:
ppublish
Résumé
Carbon and chlorine isotope effects for biotransformation of chloroform by different microbes show significant variability. Reductive dehalogenases (RDase) enzymes contain different cobamides, affecting substrate preferences, growth yields, and dechlorination rates and extent. We investigate the role of cobamide type on carbon and chlorine isotopic signals observed during reductive dechlorination of chloroform by the RDase CfrA. Microcosm experiments with two subcultures of a Dehalobacter-containing culture expressing CfrA-one with exogenous cobamide (Vitamin B
Substances chimiques
Chloroform
7V31YC746X
Vitamin B 12
P6YC3EG204
Chlorine
4R7X1O2820
Carbon Isotopes
0
Cobamides
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e1433Subventions
Organisme : Canada Research Chair
Organisme : NSERC Discovery Grants
Organisme : Agriscience Canada
Organisme : CIFAR Earth4D program
Informations de copyright
© 2024 The Author(s). MicrobiologyOpen published by John Wiley & Sons Ltd.
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