Novel insights into vancomycin-loaded calcium sulfate and negative pressure wound therapy in preventing infections in open fractures.
Calcium Sulfate
/ administration & dosage
Negative-Pressure Wound Therapy
/ methods
Humans
Vancomycin
/ administration & dosage
Fractures, Open
/ therapy
Macrophages
Male
Female
Anti-Bacterial Agents
/ administration & dosage
Middle Aged
Staphylococcal Infections
/ prevention & control
Adult
Wound Healing
/ drug effects
Surgical Wound Infection
/ prevention & control
M2
Macrophage polarization
Negative pressure wound therapy
Open fractures
Staphylococcus aureus infections
Vancomycin-Loaded Calcium Sulfate
Journal
Journal of orthopaedic surgery and research
ISSN: 1749-799X
Titre abrégé: J Orthop Surg Res
Pays: England
ID NLM: 101265112
Informations de publication
Date de publication:
29 Aug 2024
29 Aug 2024
Historique:
received:
16
04
2024
accepted:
18
07
2024
medline:
31
8
2024
pubmed:
31
8
2024
entrez:
28
8
2024
Statut:
epublish
Résumé
Open fractures are challenging due to susceptibility to Staphylococcus aureus infections. This study examines the impact of Vancomycin-Loaded Calcium Sulfate (VLCS) and negative pressure wound therapy (NPWT) on macrophage behavior in enhancing healing and infection resistance. Both VLCS and NPWT were evaluated individually and in combination to determine their effects on macrophage polarization and infection resistance in open fractures. Through single-cell RNA sequencing, genomic expressions in macrophages from open fracture patients treated with VLCS and NPWT were compared to a control group. The analysis focused on MBD2 gene changes related to macrophage polarization. Remarkable modifications in MBD2 expression in the treatment group indicate a shift towards M2 macrophage polarization. Additionally, the combined treatment group exhibited greater improvements in infection resistance and healing compared to the individual treatments. This shift suggests a healing-promoting atmosphere with improved infection resilience. VLCS and NPWT demonstrate the ability to alter macrophage behavior toward M2 polarization, which is crucial for infection prevention in open fractures. The synergistic effect of their combined use shows even greater promise in enhancing outcomes in orthopedic trauma care.
Sections du résumé
BACKGROUND
BACKGROUND
Open fractures are challenging due to susceptibility to Staphylococcus aureus infections. This study examines the impact of Vancomycin-Loaded Calcium Sulfate (VLCS) and negative pressure wound therapy (NPWT) on macrophage behavior in enhancing healing and infection resistance. Both VLCS and NPWT were evaluated individually and in combination to determine their effects on macrophage polarization and infection resistance in open fractures.
METHODS
METHODS
Through single-cell RNA sequencing, genomic expressions in macrophages from open fracture patients treated with VLCS and NPWT were compared to a control group. The analysis focused on MBD2 gene changes related to macrophage polarization.
RESULTS
RESULTS
Remarkable modifications in MBD2 expression in the treatment group indicate a shift towards M2 macrophage polarization. Additionally, the combined treatment group exhibited greater improvements in infection resistance and healing compared to the individual treatments. This shift suggests a healing-promoting atmosphere with improved infection resilience.
CONCLUSIONS
CONCLUSIONS
VLCS and NPWT demonstrate the ability to alter macrophage behavior toward M2 polarization, which is crucial for infection prevention in open fractures. The synergistic effect of their combined use shows even greater promise in enhancing outcomes in orthopedic trauma care.
Identifiants
pubmed: 39198853
doi: 10.1186/s13018-024-04931-5
pii: 10.1186/s13018-024-04931-5
doi:
Substances chimiques
Calcium Sulfate
WAT0DDB505
Vancomycin
6Q205EH1VU
Anti-Bacterial Agents
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
517Subventions
Organisme : National Outstanding Youth Science Fund Project of National Natural Science Foundation of China
ID : No. 51771069
Organisme : National Outstanding Youth Science Fund Project of National Natural Science Foundation of China
ID : No. 51771069
Organisme : National Outstanding Youth Science Fund Project of National Natural Science Foundation of China
ID : No. 51771069
Organisme : National Outstanding Youth Science Fund Project of National Natural Science Foundation of China
ID : No. 51771069
Organisme : National Outstanding Youth Science Fund Project of National Natural Science Foundation of China
ID : No. 51771069
Informations de copyright
© 2024. The Author(s).
Références
Hu R, Ren YJ, Yan L, et al. Analysis of staged treatment for Gustilo Anderson IIIB/C Open Tibial fractures. Indian J Orthop. 2018;52(4):411–7. https://doi.org/10.4103/ortho.IJOrtho_344_16 .
doi: 10.4103/ortho.IJOrtho_344_16
pubmed: 30078901
pmcid: 6055460
Maciel-Vergara G, Jensen AB, Eilenberg J. Cannibalism as a Possible Entry Route for Opportunistic Pathogenic Bacteria to Insect Hosts, Exemplified by Pseudomonas aeruginosa, a Pathogen of the Giant Mealworm Zophobas morio. Insects. 2018;9(3):88. Published 2018 Jul 24. https://doi.org/10.3390/insects9030088 .
Macneal P, Milroy C. Paronychia Drainage. In: StatPearls. Treasure Island (FL): StatPearls Publishing; June 5, 2023.
Aneja A, Kavolus MW, Teasdall RJ, et al. Does prophylactic local tobramycin injection lower open fracture infection rates? OTA Int. 2022;5(4):e210. https://doi.org/10.1097/OI9.0000000000000210 . Published 2022 Nov 16.
doi: 10.1097/OI9.0000000000000210
pubmed: 36569107
pmcid: 9782352
Rybak MJ. The pharmacokinetic and pharmacodynamic properties of Vancomycin. Clin Infect Dis. 2006;42(Suppl 1):S35–9. https://doi.org/10.1086/491712 .
doi: 10.1086/491712
pubmed: 16323118
Huang K, Lin BY, Ren HY, et al. Zhongguo Gu Shang. 2021;34(6):550–3. https://doi.org/10.12200/j.issn.1003-0034.2021.06.014 .
doi: 10.12200/j.issn.1003-0034.2021.06.014
pubmed: 34180176
Li J, Jiang X, Li H, Gelinsky M, Gu Z. Tailoring materials for modulation of macrophage fate. Adv Mater. 2021;33(12):e2004172. https://doi.org/10.1002/adma.202004172 .
doi: 10.1002/adma.202004172
pubmed: 33565154
pmcid: 9245340
Wu H, Zheng J, Xu S, et al. Mer regulates microglial/macrophage M1/M2 polarization and alleviates neuroinflammation following traumatic brain injury. J Neuroinflammation. 2021;18(1):2. https://doi.org/10.1186/s12974-020-02041-7 . Published 2021 Jan 5.
doi: 10.1186/s12974-020-02041-7
pubmed: 33402181
pmcid: 7787000
Louiselle AE, Niemiec SM, Zgheib C, Liechty KW. Macrophage polarization and diabetic wound healing. Transl Res. 2021;236:109–16. https://doi.org/10.1016/j.trsl.2021.05.006 .
doi: 10.1016/j.trsl.2021.05.006
pubmed: 34089902
Zhu GQ, Wang Y, Wang B, et al. Targeting HNRNPM inhibits Cancer Stemness and enhances Antitumor Immunity in wnt-activated Hepatocellular Carcinoma. Cell Mol Gastroenterol Hepatol. 2022;13(5):1413–47. https://doi.org/10.1016/j.jcmgh.2022.02.006 .
doi: 10.1016/j.jcmgh.2022.02.006
pubmed: 35158098
pmcid: 8938476
Wu GR, Zhou M, Wang Y, et al. Blockade of Mbd2 by siRNA-loaded liposomes protects mice against OVA-induced allergic airway inflammation via repressing M2 macrophage production. Front Immunol. 2022;13:930103. https://doi.org/10.3389/fimmu.2022.930103 . Published 2022 Aug 25.
doi: 10.3389/fimmu.2022.930103
pubmed: 36090987
pmcid: 9453648
Seidel D, Storck M, Lawall H, et al. Negative pressure wound therapy compared with standard moist wound care on diabetic foot ulcers in real-life clinical practice: results of the German DiaFu-RCT. BMJ Open. 2020;10(3):e026345. https://doi.org/10.1136/bmjopen-2018-026345 . Published 2020 Mar 24.
doi: 10.1136/bmjopen-2018-026345
pubmed: 32209619
pmcid: 7202734
Rodziewicz TL, Houseman B, Vaqar S, Hipskind JE. Medical error reduction and Prevention. StatPearls. Treasure Island (FL). Volume 12. StatPearls Publishing; 2024.
Kawase A, Takashima O, Tanaka S, Shimada H, Iwaki M. Diclofenac-Induced cytotoxicity in Direct and Indirect Co-culture of HepG2 cells with differentiated THP-1 cells. Int J Mol Sci. 2022;23(15):8660. https://doi.org/10.3390/ijms23158660 . Published 2022 Aug 4.
doi: 10.3390/ijms23158660
pubmed: 35955793
pmcid: 9368861
Gu J, Wang T, Fan G, Ma J, Hu W, Cai X. Biocompatibility of artificial bone based on Vancomycin loaded mesoporous silica nanoparticles and calcium sulfate composites. J Mater Sci Mater Med. 2016;27(4):64. https://doi.org/10.1007/s10856-016-5671-z .
doi: 10.1007/s10856-016-5671-z
pubmed: 26883948
pmcid: 4756035
Lustberg MB, Balasubramanian P, Miller B, et al. Heterogeneous atypical cell populations are present in blood of metastatic breast cancer patients. Breast Cancer Res. 2014;16(2):R23. https://doi.org/10.1186/bcr3622 . Published 2014 Mar 6.
doi: 10.1186/bcr3622
pubmed: 24602188
pmcid: 4053256
Sun W, Dong H, Balaz M, et al. snRNA-seq reveals a subpopulation of adipocytes that regulates thermogenesis. Nature. 2020;587(7832):98–102. https://doi.org/10.1038/s41586-020-2856-x .
doi: 10.1038/s41586-020-2856-x
pubmed: 33116305
Wang L, Liu Y, Dai Y, et al. Single-cell RNA-seq analysis reveals BHLHE40-driven pro-tumour neutrophils with hyperactivated glycolysis in pancreatic tumour microenvironment. Gut. 2023;72(5):958–71. https://doi.org/10.1136/gutjnl-2021-326070 .
doi: 10.1136/gutjnl-2021-326070
pubmed: 35688610
Chen K, Wang Q, Liu X, et al. Single cell RNA-Seq identifies Immune-related Prognostic Model and Key Signature-SPP1 in pancreatic ductal adenocarcinoma. Genes (Basel). 2022;13(10):1760. https://doi.org/10.3390/genes13101760 . Published 2022 Sep 29.
doi: 10.3390/genes13101760
pubmed: 36292645
Qi J, Sun H, Zhang Y et al. Single-cell and spatial analysis reveal interaction of FAP
Di Z, Zhou S, Xu G et al. Single-cell and WGCNA uncover a prognostic model and potential oncogenes in colorectal cancer. Biol Proced Online. 2022;24(1):13. Published 2022 Sep 19. https://doi.org/10.1186/s12575-022-00175-x .
Lin W, Wang Y, Chen Y, Wang Q, Gu Z, Zhu Y. Role of Calcium Signaling Pathway-Related Gene Regulatory Networks in ischemic stroke based on multiple WGCNA and single-cell analysis. Oxid Med Cell Longev. 2021;2021:8060477. https://doi.org/10.1155/2021/8060477 . Published 2021 Dec 26.
doi: 10.1155/2021/8060477
pubmed: 34987704
pmcid: 8720592
He X, Chen H, Zhong X, et al. BST2 induced macrophage M2 polarization to promote the progression of colorectal cancer. Int J Biol Sci. 2023;19(1):331–45. https://doi.org/10.7150/ijbs.72538 . Published 2023 Jan 1.
doi: 10.7150/ijbs.72538
pubmed: 36594082
pmcid: 9760448
Cahill SV, Kwon HK, Back J, et al. Locally delivered adjuvant biofilm-penetrating antibiotics rescue impaired endochondral fracture healing caused by MRSA infection. J Orthop Res. 2021;39(2):402–14. https://doi.org/10.1002/jor.24965 .
doi: 10.1002/jor.24965
pubmed: 33336805
Stavrakis AI, Zhu S, Loftin AH et al. Controlled release of Vancomycin and Tigecycline from an Orthopaedic Implant Coating prevents Staphylococcus aureus infection in an Open Fracture Animal Model. Biomed Res Int. 2019;2019:1638508. Published 2019 Dec 12. https://doi.org/10.1155/2019/1638508 .
Liu J, Jiang J, Hui X, Wang W, Fang D, Ding L. Mir-758-5p suppresses Glioblastoma Proliferation, Migration and Invasion by Targeting ZBTB20. Cell Physiol Biochem. 2018;48(5):2074–83. https://doi.org/10.1159/000492545 .
doi: 10.1159/000492545
pubmed: 30099442
Jia Q, Cao H, Shen D, et al. Quercetin protects against atherosclerosis by regulating the expression of PCSK9, CD36, PPARγ, LXRα and ABCA1. Int J Mol Med. 2019;44(3):893–902. https://doi.org/10.3892/ijmm.2019.4263 .
doi: 10.3892/ijmm.2019.4263
pubmed: 31524223
pmcid: 6658003
Zhang J, Wu J, Sun M, et al. Phospholipase C epsilon mediates cytokine cascade induced by acute disruption of epidermal permeability barrier in mice. Biochem Biophys Rep. 2020;24:100869. https://doi.org/10.1016/j.bbrep.2020.100869 . Published 2020 Dec 9.
doi: 10.1016/j.bbrep.2020.100869
pubmed: 33336085
pmcid: 7733008
Dastouri P, Helm DL, Scherer SS, Pietramaggiori G, Younan G, Orgill DP. Waveform modulation of negative-pressure wound therapy in the murine model. Plast Reconstr Surg. 2011;127(4):1460–6. https://doi.org/10.1097/PRS.0b013e31820a63cb .
doi: 10.1097/PRS.0b013e31820a63cb
pubmed: 21460654
Sen CK, Roy S, Mathew-Steiner SS, Gordillo GM. Biofilm Management in Wound Care. Plast Reconstr Surg. 2021;148(2):e275–88. https://doi.org/10.1097/PRS.0000000000008142 .
doi: 10.1097/PRS.0000000000008142
Libby P. Inflammation during the life cycle of the atherosclerotic plaque. Cardiovasc Res. 2021;117(13):2525–36. https://doi.org/10.1093/cvr/cvab303 .
doi: 10.1093/cvr/cvab303
pubmed: 34550337
pmcid: 8783385
Sapra L, Saini C, Garg B, et al. Long-term implications of COVID-19 on bone health: pathophysiology and therapeutics. Inflamm Res. 2022;71(9):1025–40. https://doi.org/10.1007/s00011-022-01616-9 .
doi: 10.1007/s00011-022-01616-9
pubmed: 35900380
pmcid: 9330992
Ujiie H. What’s new in the pathogeneses and triggering factors of bullous pemphigoid. J Dermatol. 2023;50(2):140–9. https://doi.org/10.1111/1346-8138.16654 .
doi: 10.1111/1346-8138.16654
pubmed: 36412277
Franken A, Van Mol P, Vanmassenhove S, et al. Single-cell transcriptomics identifies pathogenic T-helper 17.1 cells and pro-inflammatory monocytes in immune checkpoint inhibitor-related pneumonitis. J Immunother Cancer. 2022;10(9):e005323. https://doi.org/10.1136/jitc-2022-005323 .
doi: 10.1136/jitc-2022-005323
pubmed: 36171010
pmcid: 9528720
Voigt AP, Mullin NK, Mulfaul K, et al. Choroidal endothelial and macrophage gene expression in atrophic and neovascular macular degeneration. Hum Mol Genet. 2022;31(14):2406–23. https://doi.org/10.1093/hmg/ddac043 .
doi: 10.1093/hmg/ddac043
pubmed: 35181781
pmcid: 9307320
Duan T, Du Y, Xing C, Wang HY, Wang RF. Toll-like receptor signaling and its role in cell-mediated immunity. Front Immunol. 2022;13:812774. https://doi.org/10.3389/fimmu.2022.812774 . Published 2022 Mar 3.
doi: 10.3389/fimmu.2022.812774
pubmed: 35309296
pmcid: 8927970
Wang X, Zhao C, Zhang C et al. Increased HERV-E clone 4 – 1 expression contributes to DNA hypomethylation and IL-17 release from CD4
Cheng L, Tang Y, Chen X, et al. Deletion of MBD2 inhibits proliferation of chronic myeloid leukaemia blast phase cells. Cancer Biol Ther. 2018;19(8):676–86. https://doi.org/10.1080/15384047.2018.1450113 .
doi: 10.1080/15384047.2018.1450113
pubmed: 29565710
pmcid: 6067900
McDonald K, Vonnes C, Hartranft S. Direct care nurses and support staff thoughts and feelings about the reasons patients fall at a cancer center. J Healthc Risk Manag. 2020;40(1):17–23. https://doi.org/10.1002/jhrm.21399 .
doi: 10.1002/jhrm.21399
pubmed: 31912563
Asai M, Li Y, Spiropoulos J, et al. Galleria mellonella as an infection model for the virulent Mycobacterium tuberculosis H37Rv. Virulence. 2022;13(1):1543–57. https://doi.org/10.1080/21505594.2022.2119657 .
doi: 10.1080/21505594.2022.2119657
pubmed: 36052440
pmcid: 9481108
Njau F, Shushakova N, Schenk H et al. Calcium dobesilate reduces VEGF signaling by interfering with heparan sulfate binding site and protects from vascular complications in diabetic mice [published correction appears in PLoS One. 2020;15(12):e0244353. doi: 10.1371/journal.pone.0244353]. PLoS One. 2020;15(1):e0218494. Published 2020 Jan 14. https://doi.org/10.1371/journal.pone.0218494 .