Drug resistance and genomic variations among Mycobacterium tuberculosis isolates from The Nile Delta, Egypt.
Mycobacterium tuberculosis
/ genetics
Egypt
/ epidemiology
Humans
Antitubercular Agents
/ pharmacology
Microbial Sensitivity Tests
Whole Genome Sequencing
/ methods
Tuberculosis, Multidrug-Resistant
/ microbiology
Mutation
Adult
Genome, Bacterial
Male
Female
Drug Resistance, Bacterial
/ genetics
Drug Resistance, Multiple, Bacterial
/ genetics
Isoniazid
/ pharmacology
Genetic Variation
Middle Aged
Streptomycin
/ pharmacology
Drug resistance
Egypt
Genome-wide variations
Lineages
Tuberculosis
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
02 Sep 2024
02 Sep 2024
Historique:
received:
19
03
2024
accepted:
13
08
2024
medline:
3
9
2024
pubmed:
3
9
2024
entrez:
2
9
2024
Statut:
epublish
Résumé
Tuberculosis is a global public health concern. Earlier reports suggested the emergence of high rates of drug resistant tuberculosis in Egypt. This study included 102 isolates of Mycobacterium tuberculosis collected from two reference laboratories in Cairo and Alexandria. All clinical isolates were sub-cultured on Löwenstein-Jensen medium and analyzed using both BD BACTEC MGIT 960 SIRE Kit and standard diffusion disk assays to identify the antibiotic sensitivity profile. Extracted genomic DNA was subjected to whole genome sequencing (WGS) using Illumina platform. Isolates that belong to lineage 4 represented > 80%, while lineage 3 represented only 11% of the isolates. The percentage of drug resistance for the streptomycin, isoniazid, rifampicin and ethambutol were 31.0, 17.2, 19.5 and 20.7, respectively. Nearly 47.1% of the isolates were sensitive to the four anti-tuberculous drugs, while only one isolate was resistant to all four drugs. In addition, several new and known mutations were identified by WGS. High rates of drug resistance and new mutations were identified in our isolates. Tuberculosis control measures should focus on the spread of mono (S, I, R, E)- and double (S, E)-drug resistant strains present at higher rates throughout the whole Nile Delta, Egypt.
Identifiants
pubmed: 39223176
doi: 10.1038/s41598-024-70199-8
pii: 10.1038/s41598-024-70199-8
doi:
Substances chimiques
Antitubercular Agents
0
Isoniazid
V83O1VOZ8L
Streptomycin
Y45QSO73OB
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
20401Subventions
Organisme : US-Egypt STDF program, Cycle 19, USAID-STDF, Egypt
ID : Project ID:42693/No. 20000010565
Informations de copyright
© 2024. The Author(s).
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