Orthogonal genome editing and transcriptional activation in tomato using CRISPR-Combo systems.

Solanum lycopersicum / genetics Gene Editing / methods CRISPR-Cas Systems Transcriptional Activation / genetics Genome, Plant / genetics Plants, Genetically Modified / genetics

Journal

Plant cell reports
ISSN: 1432-203X
Titre abrégé: Plant Cell Rep
Pays: Germany
ID NLM: 9880970

Informations de publication

Date de publication:
05 Sep 2024
Historique:
received: 20 07 2024
accepted: 26 08 2024
medline: 5 9 2024
pubmed: 5 9 2024
entrez: 5 9 2024
Statut: epublish

Résumé

The CRISPR-Combo systems (Cas9-Combo and CBE-Combo) are designed for comprehensive genetic manipulation, enabling Cas9-based targeted mutagenesis or cytosine base editing with simultaneous gene activation in tomato stable lines.

Identifiants

DOI: 10.1007/s00299-024-03316-6 PMID: 39235647
pubmed: 39235647
doi: 10.1007/s00299-024-03316-6
pii: 10.1007/s00299-024-03316-6
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

227

Subventions

Organisme : Directorate for Biological Sciences
ID : IOS-2029889
Organisme : Directorate for Biological Sciences
ID : IOS-2132693
Organisme : Foundation for Food and Agriculture Research
ID : 21010111

Informations de copyright

© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.

Références

Cui L, Zheng F, Li C, Li G, Ye J, Zhang Y, Wang T, Hong Z, Ye Z, Zhang J (2024) Defective mutations in STAY-GREEN 1, PHYTOENE SYNTHASE 1, and MYB12 genes lead to formation of green ripe fruit in tomato. J Exp Bot 75(11):3322–3336. https://doi.org/10.1093/jxb/erae095
doi: 10.1093/jxb/erae095 pubmed: 38506421
Krieger U, Lippman ZB, Zamir D (2010) The flowering gene SINGLE FLOWER TRUSS drives heterosis for yield in tomato. Nat Genet 42(5):459–463. https://doi.org/10.1038/ng.550
doi: 10.1038/ng.550 pubmed: 20348958
Pan C, Qi Y (2023) CRISPR-combo–mediated orthogonal genome editing and transcriptional activation for plant breeding. Nat Protoc 18(6):1760–1794. https://doi.org/10.1038/s41596-023-00823-w
doi: 10.1038/s41596-023-00823-w pubmed: 37085666
Pan C, Xincheng Wu, Markel K, Malzahn AA, Kundagrami N, Sretenovic S, Zhang Y, Cheng Y, Shih PM, Qi Y (2021) CRISPR–Act3.0 for highly efficient multiplexed gene activation in plants. Nat Plants 7(7):942–953. https://doi.org/10.1038/s41477-021-00953-7
doi: 10.1038/s41477-021-00953-7 pubmed: 34168320
Pan C, Li G, Malzahn AA, Cheng Y, Leyson B, Sretenovic S, Gurel F, Coleman GD, Qi Y (2022) Boosting plant genome editing with a versatile CRISPR-combo system. Nature Plants 8(5):513–525. https://doi.org/10.1038/s41477-022-01151-9
doi: 10.1038/s41477-022-01151-9 pubmed: 35596077
Ren Q, Sretenovic S, Liu G, Zhong Z, Wang J, Lan H, Tang Xu et al (2021) Improved plant cytosine base editors with high editing activity, purity, and specificity. Plant Biotechnol J 19(10):2052–2068. https://doi.org/10.1111/pbi.13635
doi: 10.1111/pbi.13635 pubmed: 34042262 pmcid: 8486236
Soyk S, Müller NA, Park SJ, Schmalenbach I, Jiang Ke, Hayama R, Zhang L, Van Eck J, Jiménez-Gómez JM, Lippman ZB (2017) Variation in the flowering gene SELF PRUNING 5G promotes day-neutrality and early yield in tomato. Nat Genet 49(1):162–168. https://doi.org/10.1038/ng.3733
doi: 10.1038/ng.3733 pubmed: 27918538
Wang S, Yang Y, Chen F, Jiang J (2022) Functional diversification and molecular mechanisms of FLOWERING LOCUS T/TERMINAL FLOWER 1 family genes in horticultural plants. Mol Hortic 2(1):19. https://doi.org/10.1186/s43897-022-00039-8
doi: 10.1186/s43897-022-00039-8 pubmed: 37789396 pmcid: 10515248

Auteurs

Innocent Byiringiro (I)

Department of Plant Science and Landscape Architecture, University of Maryland, College Park, MD, USA.

Changtian Pan (C)

Department of Horticulture, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China.

Yiping Qi (Y)

Department of Plant Science and Landscape Architecture, University of Maryland, College Park, MD, USA. yiping@umd.edu.
Institute for Bioscience and Biotechnology Research, Rockville, MD, USA. yiping@umd.edu.
ORCID: 0000-0002-9556-6706

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Viridiplantae › Streptophyta › Embryophyta › Tracheobionta › Magnoliopsida › Solanales › Solanaceae › Lycopersicon esculentum › Orthogonal genome editing and transcriptional...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Génie génétique › Édition de gène › Orthogonal genome editing and transcriptional...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Épigenèse génétique › Extinction de l'expression des gènes › Systèmes CRISPR-Cas › Orthogonal genome editing and transcriptional...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Activation de la transcription › Orthogonal genome editing and transcriptional...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Génome végétal › Orthogonal genome editing and transcriptional...
questionsmedicales.fr › Stades d'organismes › Organismes génétiquement modifiés › Végétaux génétiquement modifiés › Orthogonal genome editing and transcriptional...