Construction of a thermoresponsive molecularly imprinted biomimetic hydrogel-based virus sensor and non-invasive cyclable detection of EV71.
Biomimetic
EV71
Fluorimetric detection
Hydrogel
Molecularly imprinted polymers
Thermal response
Journal
Mikrochimica acta
ISSN: 1436-5073
Titre abrégé: Mikrochim Acta
Pays: Austria
ID NLM: 7808782
Informations de publication
Date de publication:
11 Sep 2024
11 Sep 2024
Historique:
received:
21
06
2024
accepted:
28
08
2024
medline:
12
9
2024
pubmed:
12
9
2024
entrez:
11
9
2024
Statut:
epublish
Résumé
A thermoresponsive molecularly imprinted hydrogel sensor was constructed for the specific selective recognition of enterovirus 71 (EV71). Due to the introduction of the thermosensitive monomer N-isopropylacrylamide (NIPAM), when the imprinted hydrogel is incubated with the virus at 37℃, the surface specific imprinting cavity will specifically recognize and capture the target virus EV71. When the temperature rises to 45℃, the combined EV71 is rapidly released due to changes in the shape and function of the imprinted sites. The MIP hydrogel-based viral sensor developed recognized, captured, and released the target virus in a non-invasive way. The imprinting factor of the target virus was 5.2, suggesting high selectivity, and the detection limit was 7.1 fM, suggesting high sensitivity. Detection was rapid, as adsorption equilibrium was achieved within 30 min. This method provides a new sustainable avenue for the simple and rapid detection of viruses.
Identifiants
pubmed: 39261375
doi: 10.1007/s00604-024-06673-x
pii: 10.1007/s00604-024-06673-x
doi:
Substances chimiques
Hydrogels
0
Molecularly Imprinted Polymers
0
Acrylamides
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
591Subventions
Organisme : the National Natural Science Foundation of China
ID : 21775132
Organisme : the National Natural Science Foundation of China
ID : 21775132
Organisme : the National Natural Science Foundation of China
ID : 21775132
Organisme : the National Natural Science Foundation of China
ID : 21775132
Organisme : the National Natural Science Foundation of China
ID : 21775132
Organisme : the National Natural Science Foundation of China
ID : 21775132
Informations de copyright
© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.
Références
Zhu F, Liang Z, Li X, Ge H, Meng F, Mao Q, Zhang Y, Hu Y, Zhang Z, Li J, Gao F, Chen Q, Zhu Q, Chu K, Wu X, Yao X, Guo H, Chen X, Liu P, Dong Y, Li F, Shen X, Wang J (2013) Immunogenicity and safety of an enterovirus 71 vaccine in healthy Chinese children and infants: a randomised, double-blind, placebo-controlled phase 2 clinical trial. Lancet 381:1037–1045
doi: 10.1016/S0140-6736(12)61764-4
pubmed: 23352749
Liu S, Pan H, Liu P, Amer S, Chan TC, Zhan J, Huo X, Liu Y, Teng Z, Wang L, Zhuang H (2015) Comparative epidemiology and virology of fatal and nonfatal cases of hand, foot and mouth disease in mainland China from 2008 to 2014. Rev Med Virol 25:115–128
doi: 10.1002/rmv.1827
pubmed: 25704797
Chen L, Wang X, Lu W, Wu X, Li J (2016) Molecular imprinting: perspectives and applications. Chem Soc Rev 45:2137–2211
doi: 10.1039/C6CS00061D
pubmed: 26936282
Xu J, Miao H, Wang J, Pan G (2020) Molecularly imprinted synthetic antibodies: from chemical design to biomedical applications. Small 16(27):1906644
doi: 10.1002/smll.201906644
Chen H, Guo J, Wang Y, Dong W, Zhao Y, Sun L (2022) Bio-inspired imprinting materials for biomedical applications. Adv Sci 9:2202038
doi: 10.1002/advs.202202038
Song Z, Li J, Lu W, Li B, Yang G, Yi B, Arabi M, Wang X, Wang X, Ma J, Chen L (2022) Molecularly imprinted polymers based materials and their applications in chromatographic and electrophoretic separations. Trends Anal Chem 146:116504
doi: 10.1016/j.trac.2021.116504
Lu H, Xu S (2020) Dual channel ion imprinted fluorescent polymers for dual mode simultaneous chromium speciation analysis. Analyst 145:2661–2668
doi: 10.1039/D0AN00098A
pubmed: 32104830
Liu G, Ling J, Li J (2021) Extremely sensitive molecularly imprinted ECL sensor with multiple probes released from liposomes immobilized by a light-triggered click reaction. ACS Sens 6:4185–4192
doi: 10.1021/acssensors.1c01763
pubmed: 34662113
Lu H, Xu S, Guo Z, Zhao M, Liu Z (2021) Redox-responsive molecularly imprinted nanoparticles for targeted intracellular delivery of protein toward cancer therapy. ACS Nano 15:18214–18225
doi: 10.1021/acsnano.1c07166
pubmed: 34664930
Zaidi SA (2021) An overview of bio-inspired intelligent imprinted polymers for virus determination. Biosensors 11:89
doi: 10.3390/bios11030089
pubmed: 33801007
pmcid: 8004044
Cumbo A, Lorber B, Corvini PFX, Meier W, Shahgaldian PA (2013) synthetic nanomaterial for virus recognition produced by surface imprinting. Nat Commun 4:1503
doi: 10.1038/ncomms2529
pubmed: 23422671
Dinc M, Esen C, Mizaikoff B (2019) Recent advances on core-shell magnetic molecularly imprinted polymers for biomacromolecules. Trends Anal Chem 114:202–217
doi: 10.1016/j.trac.2019.03.008
Bai W, Spivak DA (2014) A double-imprinted diffraction-grating sensor based on a virus-responsive super-aptamer hydrogel derived from an impure extract. Angew Chem Int Ed 53:2095–2098
doi: 10.1002/anie.201309462
Silva MS, Tavares APM, Coelho LFL, Dias LEMF, Chambi RMC, Fonseca FG, Sale MGF, Figueiredo EC (2021) Rational selection of hidden epitopes for a molecularly imprinted electrochemical sensor in the recognition of heat-denatured dengue NS1 protein. Biosens Bioelectron 191:113419
doi: 10.1016/j.bios.2021.113419
Soufi GJ, Iravani S, Varma RS (2021) Molecularly imprinted polymers for the detection of viruses: Challenges and opportunities. Analyst 146:3087–3100
doi: 10.1039/D1AN00149C
Gast M, Sobek H, Mizaikoff B (2019) Advances in imprinting strategies for selective virus recognition a review. Trends Anal Chem 114:218–232
doi: 10.1016/j.trac.2019.03.010
Lv S, Liu Y, Xie M, Wang J, Yan X, Li Z, Dong W, Huang W (2016) Near-infrared light-responsive hydrogel for specific recognition and photothermal site-release of circulating tumor cells. ACS Nano 10:6201–6210
doi: 10.1021/acsnano.6b02208
pubmed: 27299807
Xu J, Merlier F, Avalle B, Vieillard V, DebréP HK, Bui B (2019) Molecularly imprinted polymer nanoparticles as potential synthetic antibodies for immunoprotection against HIV. ACS Appl Mater Interfaces 11:9824–9831
doi: 10.1021/acsami.8b22732
pubmed: 30758939
Liu Y, Shen T, Hu L, Gong H, Chen C, Chen X, Cai C (2017) Development of a thermosensitive molecularly imprinted polymer resonance light scattering sensor for rapid and highly selective detection of hepatitis A virus in vitro. Sensor Actuat B Chem 253:1188–1193
doi: 10.1016/j.snb.2017.07.166
Luo L, Zhang F, Chen C, Cai C (2020) Molecular imprinting resonance light scattering nanoprobes based on pH-responsive metal-organic framework for determination of hepatitis A virus. Microchim Acta 187:1–8
doi: 10.1007/s00604-020-4122-1
Wang L, Liang K, Tang L, Gong H, Chen C, Cai C (2021) Photonic and magnetic dual-responsive molecularly imprinted sensor for highly specific recognition of enterovirus 71. ACS Sens 6:3715–3723
doi: 10.1021/acssensors.1c01487
pubmed: 34641672
Wang L, Yang J, He S, Gong H, Chen C, Cai C (2022) A mild and safe gas-responsive molecularly imprinted sensor for highly specific recognition of hepatitis B virus. Sensor Actuat B Chem 366:131990
doi: 10.1016/j.snb.2022.131990
Lim SL, Ooi CW, Low LE, Tan WS, Chan ES, Ho KL, Tey BT (2020) Synthesis of poly (acrylamide)-based hydrogel for bio-sensing of hepatitis B core antigen. Mater Chem Phys 243:122578
doi: 10.1016/j.matchemphys.2019.122578
Pan B, Su P, Jin M, Huang X, Wang Z, Zhang R, Xu H, Liu W, Ye Y (2023) Ultrathin hierarchical hydrogel-carbon nanocomposite for highly-stretchable fast-response water-proof wearable humidity sensor. Mater Horiz 10:5263–5276
doi: 10.1039/D3MH01093G
pubmed: 37750039
Wang Y, Jiang X, Li X, Ding K, Liu X, Huang B, Ding G, Qu K, Sun W, Xue Z, Xu W (2023) Bionic ordered structured hydrogels: structure types, design strategies, optimization mechanism of mechanical properties and applications. Mater Horiz 10:4033–4058
doi: 10.1039/D3MH00326D
pubmed: 37522298
Vermette P, Gengenbach T, Divisekera U, Kambouris PA, Griesser HJ, Meagher L (2003) Immobilization and surface characterization of NeutrAvidin biotin-binding protein on different hydrogel interlayers. J Colloid Interface Sci 259:13–26
doi: 10.1016/S0021-9797(02)00185-6
pubmed: 12651129
Zhang X, Zhang J, Liao W, Zhang D, Dai Y, Wu C, Wen J, Zeng W (2023) Stretchable conductive hydrogels integrated with microelectronic devices for strain sensing. J Mater Chem C 11:15873–15880
doi: 10.1039/D3TC02359A
Wei Y, Zeng Q, Huang J, Hu Q, Guo X, Wang L (2018) An electro-responsive imprinted biosensor with switchable affinity toward proteins. Chem Commun 54:9163–9166
doi: 10.1039/C8CC05482G
Tamayol A, Akbari M, Zilberman Y, Comotto M, Lesha E, Serex L, Bagherifard S, Chen Y, Fu G, Ameri SK, Ruan W, Miller E, Dokmeci MR, Sonkusale S, Khademhosseini A (2016) pH-Sensing hydrogel fibers: flexible ph-sensing hydrogel fibers for epidermal applications. Adv Healthcare Mater 5:624–624
doi: 10.1002/adhm.201670027
Meng F, Ni Y, Ji S, Fu X, Wei Y, Sun J, Li Z (2017) Dual thermal-and pH-responsive polypeptide-based hydrogels. Chinese J Polym Sci 35:1243–1252
doi: 10.1007/s10118-017-1959-9
Wei Y, Zeng Q, Hu Q, Wang M, Tao J, Wang L (2018) Self-cleaned electrochemical protein imprinting biosensor basing on a thermo-responsive memory hydrogel. Biosens Bioelectron 99:136–141
doi: 10.1016/j.bios.2017.07.049
pubmed: 28750337
Wei Y, Zeng Q, Huang J, Guo X, Wang L, Wang L (2020) Preparation of gas-responsive imprinting hydrogel and their gas-driven switchable affinity for target protein recognition. ACS Appl Mater Interfaces 12:24363–24369
doi: 10.1021/acsami.0c05561
pubmed: 32366087
Chen D, Timperman AT (2022) Analyte enrichment via ion concentration polarization with hydrogel plugs polymerized in PDMS microchannels by a facile and comprehensive method for improved polymerization. Anal Chem 94:15586–15594
doi: 10.1021/acs.analchem.2c01394
pubmed: 36318671
pmcid: 10284069
Yin S, Xu Y, Wang Z, Wei Z, Xu T, Zhao W, Zhao C (2022) Molecularly-imprinted hydrogel beads via self-sacrificing micro-reactors as safe and selective bilirubin adsorbents. J Mater Chem B 10:2534–2543
doi: 10.1039/D1TB01895G
pubmed: 34786576
Sun X, Jin X, Pan W, Guo E, Liu W, Li D, Lu K, Si S, Zhang L, Shi Y, Li Q, Jia Z, Wang J (2016) Highly luminescent carbon dots synthesized by microwave-assisted pyrolysis and evaluation of their toxicity to Physa acuta. J Nanosci Nanotechnol 16:648–653
doi: 10.1166/jnn.2016.11112
pubmed: 27398502
Zhou M, Zhou Z, Gong A, Zhang Y, Li Q (2015) Synthesis of highly photoluminescent carbon dots via citric acid and Tris for iron (III) ions sensors and bioimaging. Talanta 143:107–113
doi: 10.1016/j.talanta.2015.04.015
pubmed: 26078136
Tang L, Liang K, Wang L, Chen C, Cai C, Gong H (2022) Construction of an ultrasensitive molecularly imprinted virus sensor based on an “explosive” secondary amplification strategy for the visual detection of viruses. Anal Chem 94:13879–13888
doi: 10.1021/acs.analchem.2c02588
pubmed: 36170349
Luo L, Zhang F, Chen C, Cai C (2019) Visual simultaneous detection of hepatitis A and B viruses based on a multifunctional molecularly imprinted fluorescence sensor. Anal Chem 91:15748–15756
doi: 10.1021/acs.analchem.9b04001
pubmed: 31718158
Yang B, Gong H, Chen C, Chen X, Cai C (2017) A virus resonance light scattering sensor based on mussel-inspired molecularly imprinted polymers for high sensitive and high selective detection of Hepatitis A Virus. Biosens Bioelectron 87:679–685
doi: 10.1016/j.bios.2016.08.087
pubmed: 27631682
He K, Chen C, Liang C, Liu C, Yang B, Chen X, Cai C (2016) Highly selective recognition and fluorescent detection of JEV via virus-imprinted magnetic silicon microspheres. Sensor Actuat B Chem 233:607–614
doi: 10.1016/j.snb.2016.04.127