Evaluation of the Antimicrobial Activity of Triple Enzyme-Embedded Organic-Inorganic Hybrid Nanoflowers (hNFs) in Comparison with Powerful Antimicrobial Agent Chitosan.
Chitosan
/ chemistry
Lipase
/ chemistry
Staphylococcus aureus
/ drug effects
Escherichia coli
/ drug effects
alpha-Amylases
/ chemistry
Peptide Hydrolases
/ chemistry
Microbial Sensitivity Tests
Anti-Bacterial Agents
/ pharmacology
Nanostructures
/ chemistry
Anti-Infective Agents
/ pharmacology
Pancreatin
/ chemistry
Enzymes, Immobilized
/ chemistry
Journal
Current microbiology
ISSN: 1432-0991
Titre abrégé: Curr Microbiol
Pays: United States
ID NLM: 7808448
Informations de publication
Date de publication:
17 Sep 2024
17 Sep 2024
Historique:
received:
19
05
2024
accepted:
04
09
2024
medline:
18
9
2024
pubmed:
17
9
2024
entrez:
17
9
2024
Statut:
epublish
Résumé
Organic-inorganic hybrid nanoflowers (hNFs) have high stability, reusability, low production cost, and overcome substrate/product inhibition. Antimicrobial activity of various hNFs has been reported to overcome environmental microbial contaminations and infections, which are considered major public health problems. α-amylase, protease, and lipase are the most common industrial enzymes exerting antimicrobial activity; therefore, we synthesized triple enzyme (α-amylase, protease, and lipase)-embedded hNFs by using pancreatin to evaluate their antimicrobial activity in comparison with one of the most potent antimicrobial polymer chitosan. The broad spectrum of the antimicrobial properties of chitosan is used in industrial products, including cosmetics, food, agriculture, pharmaceuticals, and textiles. SEM analysis, thermogravimetric analysis (TGA), and the degree of deacetylation (%DD) were performed for chitosan characterization, where SEM, FTIR, EDX, and XRD analyses were performed for the characterization of hNFs. The catalytic activity of pancreatin and hNFs was evaluated by measuring lipase, α-amylase, and protease enzyme activities at 37 °C. Antibacterial activities of hNFs, pancreatin, and chitosan were tested on gram-positive (Staphylococcus aureus) and gram-negative (Escherichia coli) bacteria, compared to the pancreatin and chitosan via agar and broth dilution methods. hNFs showed enhanced catalytic activity for protease, lipase, and α-amylase compared to pancreatin at different pH values (pH 8, 9). hNFs showed catalytic activity after being washed and reused up to six times, indicating their reusability and recoverability. hNFs showed significant antimicrobial activity, such as chitosan, Staphylococcus aureus, and Escherichia coli, compared to pancreatin. Our novel hNFs can be used to develop antimicrobial technologies to fight against environmental microbial contaminations and antibiotic resistance-driven environmental pathogens.
Identifiants
pubmed: 39287689
doi: 10.1007/s00284-024-03884-6
pii: 10.1007/s00284-024-03884-6
doi:
Substances chimiques
Chitosan
9012-76-4
Lipase
EC 3.1.1.3
alpha-Amylases
EC 3.2.1.1
Peptide Hydrolases
EC 3.4.-
Anti-Bacterial Agents
0
Anti-Infective Agents
0
Pancreatin
8049-47-6
Enzymes, Immobilized
0
Types de publication
Journal Article
Comparative Study
Langues
eng
Sous-ensembles de citation
IM
Pagination
359Informations de copyright
© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
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