Microbial community interactions on a chip.

Bacillus cereus Microbial Interactions / physiology Microbiota / physiology Anti-Bacterial Agents / pharmacology Vancomycin / pharmacology Rhizosphere Gentamicins / pharmacology Lab-On-A-Chip Devices Green Fluorescent Proteins / metabolism
antibiotics fabricated microbial ecosystems microbial communities microbial interactions microfluidics

Journal

Proceedings of the National Academy of Sciences of the United States of America
ISSN: 1091-6490
Titre abrégé: Proc Natl Acad Sci U S A
Pays: United States
ID NLM: 7505876

Informations de publication

Date de publication:
24 Sep 2024
Historique:
medline: 17 9 2024
pubmed: 17 9 2024
entrez: 17 9 2024
Statut: ppublish

Résumé

Multispecies microbial communities drive most ecosystems on Earth. Chemical and biological interactions within these communities can affect the survival of individual members and the entire community. However, the prohibitively high number of possible interactions within a microbial community has made the characterization of factors that influence community development challenging. Here, we report a Microbial Community Interaction (µCI) device to advance the systematic study of chemical and biological interactions within a microbial community. The µCI creates a combinatorial landscape made up of an array of triangular wells interconnected with circular wells, which each contains either a different chemical or microbial strain, generating chemical gradients and revealing biological interactions.

Identifiants

DOI: 10.1073/pnas.2403510121 PMID: 39288179
pubmed: 39288179
doi: 10.1073/pnas.2403510121
doi:

Substances chimiques

Anti-Bacterial Agents 0
Vancomycin 6Q205EH1VU
Gentamicins 0
Green Fluorescent Proteins 147336-22-9

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

e2403510121

Subventions

Organisme : HHS | NIH (NIH)
ID : P30CA014520
Organisme : NIGMS NIH HHS
ID : R35 GM124774
Pays : United States
Organisme : NIBIB NIH HHS
ID : R01 EB030340
Pays : United States
Organisme : U.S. Army Research Laboratory
ID : W911NF1910269
Organisme : UW | Office of the Vice Chancellor for Research and Graduate Education, University of Wisconsin-Madison (VCRGE, UW)
ID : N/A
Organisme : State of Wisconsin
ID : N/A

Déclaration de conflit d'intérêts

Competing interests statement:D.J.B. holds equity in Bellbrook Labs LLC, Tasso Inc., Salus Discovery LLC, Lynx Biosciences Inc., Stacks to the Future LLC, Onexio Biosystems LLC, Navitro Biosciences LLC, and Flambeau Diagnostics LLC. J.H. holds equity in Wacasa Pharmaceuticals, Inc. A patent application (US011185860B2) was filed through the Wisconsin Alumni Research Foundation for the device described in this work.

Auteurs

Duane S Juang (DS)

Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI 53706.
ORCID: 0000-0002-6832-4277

Wren E Wightman (WE)

Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI 53706.
ORCID: 0000-0001-8468-470X

Gabriel L Lozano (GL)

Wisconsin Institute for Discovery, University of Wisconsin-Madison, Madison, WI 53715.
Department of Plant Pathology, University of Wisconsin-Madison, Madison, WI 53706.
ORCID: 0000-0001-9327-4470

Terry D Juang (TD)

Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI 53706.

Layla J Barkal (LJ)

Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI 53706.
ORCID: 0000-0002-4734-7197

Jiaquan Yu (J)

Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI 53706.

Manuel F Garavito (MF)

Wisconsin Institute for Discovery, University of Wisconsin-Madison, Madison, WI 53715.
Department of Plant Pathology, University of Wisconsin-Madison, Madison, WI 53706.

Amanda Hurley (A)

Wisconsin Institute for Discovery, University of Wisconsin-Madison, Madison, WI 53715.
Department of Plant Pathology, University of Wisconsin-Madison, Madison, WI 53706.
ORCID: 0000-0002-8322-4143

Ophelia S Venturelli (OS)

Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706.
Department of Bacteriology, University of Wisconsin-Madison, Madison, WI 53706.
Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, WI 53706.
ORCID: 0000-0003-2200-1963

Jo Handelsman (J)

Wisconsin Institute for Discovery, University of Wisconsin-Madison, Madison, WI 53715.
Department of Plant Pathology, University of Wisconsin-Madison, Madison, WI 53706.

David J Beebe (DJ)

Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI 53706.
Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, WI 53705.
Carbone Cancer Center, University of Wisconsin-Madison, Madison, WI 53706.
ORCID: 0000-0002-0415-9006

Articles similaires

Vancomycin-associated DRESS demonstrates delay in AST abnormalities.

Ahmed Hussein, Kateri L Schoettinger, Jourdan Hydol-Smith et al.
1.00
Humans Drug Hypersensitivity Syndrome Vancomycin Female Male

Management of the infected total elbow arthroplasty.

Joaquin Sanchez-Sotelo
1.00
Humans Arthroplasty, Replacement, Elbow Prosthesis-Related Infections Debridement Anti-Bacterial Agents

Planting density effect on poplar growth traits and soil nutrient availability, and response of microbial community, assembly and function.

Rongye Qiao, Zhen Song, Yinglong Chen et al.
1.00
Populus Soil Microbiology Soil Microbiota Fungi

Comparative genomic analysis and characterization of novel high-quality draft genomes from the coal metagenome.

Arunmozhi Bharathi Achudhan, Lilly M Saleena
1.00
Coal Metagenome Phylogeny Bacteria Genome, Bacterial

Classifications MeSH

questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Bâtonnets à Gram positif › Bâtonnets sporulés à Gram positif › Bacillaceae › Bacillus › Bacillus cereus › Microbial community interactions on a chip.
questionsmedicales.fr › Phénomènes microbiologiques › Interactions microbiennes › Microbial community interactions on a chip.
questionsmedicales.fr › Environnement et santé publique › Environnement › Écosystème › Biodiversité › Biote › Microbiote › Microbial community interactions on a chip.
questionsmedicales.fr › Actions chimiques et utilisations › Actions pharmacologiques › Utilisations thérapeutiques › Anti-infectieux › Antibactériens › Microbial community interactions on a chip.
questionsmedicales.fr › Acides aminés, peptides et protéines › Peptides › Glycopeptides › Vancomycine › Microbial community interactions on a chip.
questionsmedicales.fr › Environnement et santé publique › Environnement › Écosystème › Rhizosphère › Microbial community interactions on a chip.
questionsmedicales.fr › Glucides › Hétérosides › Aminosides › Gentamicine › Microbial community interactions on a chip.
questionsmedicales.fr › Équipement et fournitures › Équipement et fournitures électriques › Laboratoires sur puces › Microbial community interactions on a chip.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines luminescentes › Protéines à fluorescence verte › Microbial community interactions on a chip.