Methylation alterations of imprinted genes in different placental diseases.
Humans
Female
Pregnancy
DNA Methylation
/ genetics
Genomic Imprinting
/ genetics
Insulin-Like Growth Factor II
/ genetics
Pre-Eclampsia
/ genetics
Adult
GTP-Binding Protein alpha Subunits, Gs
/ genetics
Placenta
/ metabolism
Epigenesis, Genetic
/ genetics
Hydrocortisone
/ blood
Placenta Diseases
/ genetics
Oxidative Stress
/ genetics
Fetal Blood
/ chemistry
Chromogranins
Proteins
Potassium Channels, Voltage-Gated
Imprinted genes
Methylation
Placenta accreta spectrum disorders
Pre-eclampsia
Journal
Clinical epigenetics
ISSN: 1868-7083
Titre abrégé: Clin Epigenetics
Pays: Germany
ID NLM: 101516977
Informations de publication
Date de publication:
18 Sep 2024
18 Sep 2024
Historique:
received:
24
05
2024
accepted:
30
08
2024
medline:
19
9
2024
pubmed:
19
9
2024
entrez:
18
9
2024
Statut:
epublish
Résumé
Imprinted genes play important functions in placentation and pregnancy; however, research on their roles in different placental diseases is limited. It is believed that epigenetic alterations, such as DNA methylation, of placental imprinting genes may contribute to the different pathological features of severe placental diseases, such as pre-eclampsia (PE) and placenta accreta spectrum disorders (PAS). In this study, we conducted a comparative analysis of the methylation and expression of placental imprinted genes between PE and PAS using bisulfite sequencing polymerase chain reaction (PCR) and quantitative PCR, respectively. Additionally, we assessed oxidative damage of placental DNA by determining 8-hydroxy-2'-deoxyguanosine levels and fetal growth by determining insulin-like growth factor 2 (IGF2) and cortisol levels in the umbilical cord blood using enzyme-linked immunosorbent assay. Our results indicated that methylation and expression of potassium voltage-gated channel subfamily Q member 1, GNAS complex locus, mesoderm specific transcript, and IGF2 were significantly altered in both PE and PAS placentas. Additionally, our results revealed that the maternal imprinted genes were significantly over-expressed in PE and significantly under-expressed in PAS compared with a normal pregnancy. Moreover, DNA oxidative damage was elevated and positively correlated with IGF2 DNA methylation in both PE and PAS placentas, and cortisol and IGF2 levels were significantly decreased in PE and PAS. This study suggested that DNA methylation and expression of imprinted genes are aberrant in both PE and PAS placentas and that PE and PAS have different methylation profiles, which may be linked to their unique pathogenesis.
Sections du résumé
BACKGROUND
BACKGROUND
Imprinted genes play important functions in placentation and pregnancy; however, research on their roles in different placental diseases is limited. It is believed that epigenetic alterations, such as DNA methylation, of placental imprinting genes may contribute to the different pathological features of severe placental diseases, such as pre-eclampsia (PE) and placenta accreta spectrum disorders (PAS).
RESULTS
RESULTS
In this study, we conducted a comparative analysis of the methylation and expression of placental imprinted genes between PE and PAS using bisulfite sequencing polymerase chain reaction (PCR) and quantitative PCR, respectively. Additionally, we assessed oxidative damage of placental DNA by determining 8-hydroxy-2'-deoxyguanosine levels and fetal growth by determining insulin-like growth factor 2 (IGF2) and cortisol levels in the umbilical cord blood using enzyme-linked immunosorbent assay. Our results indicated that methylation and expression of potassium voltage-gated channel subfamily Q member 1, GNAS complex locus, mesoderm specific transcript, and IGF2 were significantly altered in both PE and PAS placentas. Additionally, our results revealed that the maternal imprinted genes were significantly over-expressed in PE and significantly under-expressed in PAS compared with a normal pregnancy. Moreover, DNA oxidative damage was elevated and positively correlated with IGF2 DNA methylation in both PE and PAS placentas, and cortisol and IGF2 levels were significantly decreased in PE and PAS.
CONCLUSIONS
CONCLUSIONS
This study suggested that DNA methylation and expression of imprinted genes are aberrant in both PE and PAS placentas and that PE and PAS have different methylation profiles, which may be linked to their unique pathogenesis.
Identifiants
pubmed: 39294759
doi: 10.1186/s13148-024-01738-3
pii: 10.1186/s13148-024-01738-3
doi:
Substances chimiques
Insulin-Like Growth Factor II
67763-97-7
IGF2 protein, human
0
GTP-Binding Protein alpha Subunits, Gs
EC 3.6.5.1
GNAS protein, human
EC 3.6.1.-
Hydrocortisone
WI4X0X7BPJ
mesoderm specific transcript protein
0
KCNQ1OT1 long non-coding RNA, human
0
Chromogranins
0
Proteins
0
Potassium Channels, Voltage-Gated
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
132Subventions
Organisme : National Key Research and Development Program of China
ID : 2022YFC2704500
Organisme : Guangzhou Science, Technology and Innovation Commission
ID : SL2022A03J00860
Informations de copyright
© 2024. The Author(s).
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